Key features and details
- Rabbit polyclonal to Angiopoietin 1 + Angiopoietin 2
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-Angiopoietin 1 + Angiopoietin 2 antibody
DescriptionRabbit polyclonal to Angiopoietin 1 + Angiopoietin 2
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Rat, Human
- WB: Rat brain tissue lysate. ICC: PC12 cells.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservatives: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate, 2.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab65835 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 57 kDa).|
- AGP1 antibody
- AGPT antibody
- AGPT2 antibody
Anti-Angiopoietin 1 + Angiopoietin 2 antibody (ab65835) at 2 µg/ml + rat brain tissue lysate
Predicted band size: 57 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
ICC/IF image of ab65835 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65835, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab65835 has been referenced in 14 publications.
- Lun W et al. MiR-218 regulates epithelial-mesenchymal transition and angiogenesis in colorectal cancer via targeting CTGF. Cancer Cell Int 18:83 (2018). PubMed: 29977158
- Prapansilp P et al. A clinicopathological correlation of the expression of the angiopoietin-Tie-2 receptor pathway in the brain of adults with Plasmodium falciparum malaria. Malar J 12:50 (2013). IHC-P ; Human . PubMed: 23383853
- Chaitanya GV et al. Inflammation induces neuro-lymphatic protein expression in multiple sclerosis brain neurovasculature. J Neuroinflammation 10:125 (2013). WB, IHC-P ; Mouse, Human . PubMed: 24124909
- Milton I et al. Age-dependent lethality in novel transgenic mouse models of central nervous system arteriovenous malformations. Stroke 43:1432-5 (2012). IHC-Fr ; Mouse . PubMed: 22328553
- MacMillan CJ et al. Bevacizumab diminishes experimental autoimmune encephalomyelitis by inhibiting spinal cord angiogenesis and reducing peripheral T-cell responses. J Neuropathol Exp Neurol 71:983-99 (2012). Mouse . PubMed: 23037326