Anti-Angiopoietin 1 antibody (ab133425)

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Key features and details

  • Goat polyclonal to Angiopoietin 1
  • Suitable for: WB, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Angiopoietin 1 antibody
    See all Angiopoietin 1 primary antibodies

  • Description

    Goat polyclonal to Angiopoietin 1

  • Host species

    Goat

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Dog, Pig, a wide range of other species

  • Immunogen

    Synthetic peptide corresponding to Human Angiopoietin 1 aa 76-85 (internal sequence) (Cysteine residue).
    Sequence:

    C-PDFSSQKLQH


    Database link: Q15389

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human lung, respiratory epithelium tissue; Transfected HEK293 lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab133425 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 57 kDa.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiopoietin 1 antibody (ab133425)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiopoietin 1 antibody (ab133425)
    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung, respiratory epithelium tissue labelling Angiopoietin 1 with ab133425 antibody at 2.5µg/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Western blot - Anti-Angiopoietin 1 antibody (ab133425)
    Western blot - Anti-Angiopoietin 1 antibody (ab133425)
    Lane 1 : Anti-Angiopoietin 1 antibody (ab133425) at 1 µg/ml
    Lane 2 : Anti-Angiopoietin 1 antibody (ab133425) at 1 mg/ml
    Lane 3 : anti-DYKDDDDK Tag antibody at 1/1000 dilution

    Lanes 1 & 3 : HEK293 lysate, overexpressing Human Angiopoietin 1 with DYKDDDDK tag
    Lane 2 : HEK293 lysate, mock-transfected

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 57 kDa

References

Publishing research using ab133425? Please let us know so that we can cite the reference in this datasheet.

ab133425 has been referenced in 4 publications.

  • Cui J  et al. Enhancement of Angiogenesis by Ultrasound-Targeted Microbubble Destruction Combined with Nuclear Localization Signaling Peptides in Canine Myocardial Infarction. Biomed Res Int 2017:9390565 (2017). PubMed: 29259991
  • Cao S  et al. Enhanced effect of nuclear localization signal peptide during ultrasound-targeted microbubble destruction-mediated gene transfection. Mol Med Rep 16:565-572 (2017). WB ; Human . PubMed: 28586046
  • Deng Q  et al. Improving the efficacy of therapeutic angiogenesis by UTMD-mediated Ang-1 gene delivery to the infarcted myocardium. Int J Mol Med 36:335-44 (2015). WB . PubMed: 26035181
  • Xia YP  et al. Recombinant human sonic hedgehog protein regulates the expression of ZO-1 and occludin by activating angiopoietin-1 in stroke damage. PLoS One 8:e68891 (2013). WB ; Rat . PubMed: 23894369

Customer reviews and Q&As

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Angiopoietin 1 antibody

 Average
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (vessel in small intestine)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrat
Permeabilization
Yes - tris buffered saline with tween
Specification
vessel in small intestine
Blocking step
Normal horse serum as blocking agent for 20 minute(s) · Concentration: 20% · Temperature: 20°C
Fixative
Formaldehyde
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Miss. Katharina Kaiser

Verified customer

Submitted Oct 19 2019

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