Anti-Angiopoietin 1 antibody (ab133425)
Key features and details
- Goat polyclonal to Angiopoietin 1
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Angiopoietin 1 antibody
See all Angiopoietin 1 primary antibodies -
Description
Goat polyclonal to Angiopoietin 1 -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Dog, Pig, a wide range of other species
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Immunogen
Synthetic peptide corresponding to Human Angiopoietin 1 aa 76-85 (internal sequence) (Cysteine residue).
Sequence:C-PDFSSQKLQH
Database link: Q15389 -
Positive control
- Human lung, respiratory epithelium tissue; Transfected HEK293 lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133425 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 57 kDa.
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| IHC-P | (1) |
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 57 kDa. |
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IHC-P
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development. -
Sequence similarities
Contains 1 fibrinogen C-terminal domain. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 403656 Dog
- Entrez Gene: 284 Human
- Entrez Gene: 11600 Mouse
- Entrez Gene: 397009 Pig
- Entrez Gene: 89807 Rat
- Omim: 601667 Human
- SwissProt: Q60FC1 Dog
- SwissProt: Q15389 Human
see all -
Alternative names
- AGP 1 antibody
- AGP1 antibody
- AGPT antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiopoietin 1 antibody (ab133425)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung, respiratory epithelium tissue labelling Angiopoietin 1 with ab133425 antibody at 2.5µg/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Western blot - Anti-Angiopoietin 1 antibody (ab133425)Lane 1 : Anti-Angiopoietin 1 antibody (ab133425) at 1 µg/ml
Lane 2 : Anti-Angiopoietin 1 antibody (ab133425) at 1 mg/ml
Lane 3 : anti-DYKDDDDK Tag antibody at 1/1000 dilution
Lanes 1 & 3 : HEK293 lysate, overexpressing Human Angiopoietin 1 with DYKDDDDK tag
Lane 2 : HEK293 lysate, mock-transfected
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 57 kDa
Protocols
Datasheets and documents
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Datasheet download
References (6)
ab133425 has been referenced in 6 publications.
- Di Pietro M et al. Metformin has a direct effect on ovarian cells that is dependent on organic cation transporters. Mol Cell Endocrinol 499:110591 (2020). PubMed: 31546019
- Kumar H et al. Elevated TRPV4 Levels Contribute to Endothelial Damage and Scarring in Experimental Spinal Cord Injury. J Neurosci 40:1943-1955 (2020). PubMed: 31974206
- Cui J et al. Enhancement of Angiogenesis by Ultrasound-Targeted Microbubble Destruction Combined with Nuclear Localization Signaling Peptides in Canine Myocardial Infarction. Biomed Res Int 2017:9390565 (2017). PubMed: 29259991
- Cao S et al. Enhanced effect of nuclear localization signal peptide during ultrasound-targeted microbubble destruction-mediated gene transfection. Mol Med Rep 16:565-572 (2017). WB ; Human . PubMed: 28586046
- Deng Q et al. Improving the efficacy of therapeutic angiogenesis by UTMD-mediated Ang-1 gene delivery to the infarcted myocardium. Int J Mol Med 36:335-44 (2015). WB . PubMed: 26035181
- Xia YP et al. Recombinant human sonic hedgehog protein regulates the expression of ZO-1 and occludin by activating angiopoietin-1 in stroke damage. PLoS One 8:e68891 (2013). WB ; Rat . PubMed: 23894369
