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  1. Link

    angiopoietin-1-antibody-ab133425.pdf

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Cardiovascular Angiogenesis Growth Factors Angiopoietin
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Anti-Angiopoietin 1 antibody (ab133425)

  • Datasheet
Reviews (1) Submit a question References (6)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiopoietin 1 antibody (ab133425)
  • Western blot - Anti-Angiopoietin 1 antibody (ab133425)

Key features and details

  • Goat polyclonal to Angiopoietin 1
  • Suitable for: WB, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Angiopoietin 1 antibody [EPR2888(N)] (ab183701)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Angiopoietin 1 antibody
    See all Angiopoietin 1 primary antibodies
  • Description

    Goat polyclonal to Angiopoietin 1
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Dog, Pig, a wide range of other species
  • Immunogen

    Synthetic peptide corresponding to Human Angiopoietin 1 aa 76-85 (internal sequence) (Cysteine residue).
    Sequence:

    C-PDFSSQKLQH


    Database link: Q15389
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human lung, respiratory epithelium tissue; Transfected HEK293 lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • Angiopoietin
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Recombinant Protein

    • Recombinant Human Angiopoietin 1 protein (denatured) (ab113163)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133425 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 57 kDa.
IHC-P (1)
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 57 kDa.
IHC-P
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development.
  • Sequence similarities

    Contains 1 fibrinogen C-terminal domain.
  • Post-translational
    modifications

    Glycosylated.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession Q15389 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 403656 Dog
    • Entrez Gene: 284 Human
    • Entrez Gene: 11600 Mouse
    • Entrez Gene: 397009 Pig
    • Entrez Gene: 89807 Rat
    • Omim: 601667 Human
    • SwissProt: Q60FC1 Dog
    • SwissProt: Q15389 Human
    • SwissProt: O08538 Mouse
    • SwissProt: Q9BDY8 Pig
    • SwissProt: O35460 Rat
    • Unigene: 369675 Human
    • Unigene: 309336 Mouse
    • Unigene: 161953 Rat
    see all
  • Alternative names

    • AGP 1 antibody
    • AGP1 antibody
    • AGPT antibody
    • ANG 1 antibody
    • ANG antibody
    • ANG-1 antibody
    • ANG1 antibody
    • Angiopoietin-1 antibody
    • Angiopoietin1 antibody
    • ANGP1_HUMAN antibody
    • ANGPT 1 antibody
    • Angpt1 antibody
    • KIAA0003 antibody
    • OTTHUMP00000227592 antibody
    • OTTHUMP00000227599 antibody
    • OTTHUMP00000227600 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiopoietin 1 antibody (ab133425)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiopoietin 1 antibody (ab133425)
    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung, respiratory epithelium tissue labelling Angiopoietin 1 with ab133425 antibody at 2.5µg/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Western blot - Anti-Angiopoietin 1 antibody (ab133425)
    Western blot - Anti-Angiopoietin 1 antibody (ab133425)
    Lane 1 : Anti-Angiopoietin 1 antibody (ab133425) at 1 µg/ml
    Lane 2 : Anti-Angiopoietin 1 antibody (ab133425) at 1 mg/ml
    Lane 3 : anti-DYKDDDDK Tag antibody at 1/1000 dilution

    Lanes 1 & 3 : HEK293 lysate, overexpressing Human Angiopoietin 1 with DYKDDDDK tag
    Lane 2 : HEK293 lysate, mock-transfected

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 57 kDa

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (6)

Publishing research using ab133425? Please let us know so that we can cite the reference in this datasheet.

ab133425 has been referenced in 6 publications.

  • Di Pietro M  et al. Metformin has a direct effect on ovarian cells that is dependent on organic cation transporters. Mol Cell Endocrinol 499:110591 (2020). PubMed: 31546019
  • Kumar H  et al. Elevated TRPV4 Levels Contribute to Endothelial Damage and Scarring in Experimental Spinal Cord Injury. J Neurosci 40:1943-1955 (2020). PubMed: 31974206
  • Cui J  et al. Enhancement of Angiogenesis by Ultrasound-Targeted Microbubble Destruction Combined with Nuclear Localization Signaling Peptides in Canine Myocardial Infarction. Biomed Res Int 2017:9390565 (2017). PubMed: 29259991
  • Cao S  et al. Enhanced effect of nuclear localization signal peptide during ultrasound-targeted microbubble destruction-mediated gene transfection. Mol Med Rep 16:565-572 (2017). WB ; Human . PubMed: 28586046
  • Deng Q  et al. Improving the efficacy of therapeutic angiogenesis by UTMD-mediated Ang-1 gene delivery to the infarcted myocardium. Int J Mol Med 36:335-44 (2015). WB . PubMed: 26035181
  • Xia YP  et al. Recombinant human sonic hedgehog protein regulates the expression of ZO-1 and occludin by activating angiopoietin-1 in stroke damage. PLoS One 8:e68891 (2013). WB ; Rat . PubMed: 23894369

Customer reviews and Q&As

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Angiopoietin 1 antibody

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (vessel in small intestine)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrat
Permeabilization
Yes - tris buffered saline with tween
Specification
vessel in small intestine
Blocking step
Normal horse serum as blocking agent for 20 minute(s) · Concentration: 20% · Temperature: 20°C
Fixative
Formaldehyde
Read More

MISS. Katharina Kaiser

Verified customer

Submitted Oct 19 2019

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