Overview

  • Product name

    Anti-Angiopoietin 1 antibody
    See all Angiopoietin 1 primary antibodies
  • Description

    Goat polyclonal to Angiopoietin 1
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Dog, Pig, a wide range of other species
  • Immunogen

    Synthetic peptide corresponding to Human Angiopoietin 1 aa 76-85 (internal sequence) (Cysteine residue).
    Sequence:

    C-PDFSSQKLQH


    Database link: Q15389

  • Positive control

    • Human lung, respiratory epithelium tissue; Transfected HEK293 lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab133425 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 57 kDa.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung, respiratory epithelium tissue labelling Angiopoietin 1 with ab133425 antibody at 2.5µg/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Lane 1 : Anti-Angiopoietin 1 antibody (ab133425) at 1 µg/ml
    Lane 2 : Anti-Angiopoietin 1 antibody (ab133425) at 1 mg/ml
    Lane 3 : anti-DYKDDDDK Tag antibody at 1/1000 dilution

    Lanes 1 & 3 : HEK293 lysate, overexpressing Human Angiopoietin 1 with DYKDDDDK tag
    Lane 2 : HEK293 lysate, mock-transfected

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 57 kDa

References

This product has been referenced in:

  • Cui J  et al. Enhancement of Angiogenesis by Ultrasound-Targeted Microbubble Destruction Combined with Nuclear Localization Signaling Peptides in Canine Myocardial Infarction. Biomed Res Int 2017:9390565 (2017). Read more (PubMed: 29259991) »
  • Cao S  et al. Enhanced effect of nuclear localization signal peptide during ultrasound-targeted microbubble destruction-mediated gene transfection. Mol Med Rep 16:565-572 (2017). WB ; Human . Read more (PubMed: 28586046) »
See all 4 Publications for this product

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