Recombinant Anti-Angiopoietin 1 antibody [EPR2888(N)] (ab183701)
![Western blot - Anti-Angiopoietin 1 antibody [EPR2888(N)] (ab183701)](https://www.abcam.com/ps/products/183/ab183701/Images/ab183701-333622-anti-angiopoietin-1-antibody-epr2888n-western-blot.jpg "Western blot - Anti-Angiopoietin 1 antibody [EPR2888(N)] (ab183701)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2888(N)] to Angiopoietin 1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Angiopoietin 1 antibody [EPR2888(N)]
See all Angiopoietin 1 primary antibodies -
Description
Rabbit monoclonal [EPR2888(N)] to Angiopoietin 1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: U87-MG, HeLa, C6, RAW 264.7, K562, and ECV-304 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2888(N) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183701 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa).
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| Notes |
|---|
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WB
1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa). |
Target
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Function
Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development. -
Sequence similarities
Contains 1 fibrinogen C-terminal domain. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 284 Human
- Entrez Gene: 11600 Mouse
- Entrez Gene: 89807 Rat
- Omim: 601667 Human
- SwissProt: Q15389 Human
- SwissProt: O08538 Mouse
- SwissProt: O35460 Rat
- Unigene: 369675 Human
see all -
Alternative names
- AGP 1 antibody
- AGP1 antibody
- AGPT antibody
see all
Images
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Western blot - Anti-Angiopoietin 1 antibody [EPR2888(N)] (ab183701)All lanes : Anti-Angiopoietin 1 antibody [EPR2888(N)] (ab183701) at 1/10000 dilution (Purified)
Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates
Lane 2 : C6 (Rat glial tumor glial cell) whole cell lysates
Lane 3 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
Lane 4 : Human brain lysates
Lane 5 : Mouse brain lysates
Lane 6 : Rat brain lysates
Lane 7 : Human heart lysates
Lane 8 : Mouse heart lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?This antibody is unsuitable to detect tissue lysate samples.
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![Western blot - Anti-Angiopoietin 1 antibody [EPR2888(N)] (ab183701)](https://www.abcam.com/ps/products/183/ab183701/Images/ab183701-215704-anti-angiopoietin-1-antibody-epr2888n-western-blot.jpg)
Western blot - Anti-Angiopoietin 1 antibody [EPR2888(N)] (ab183701)All lanes : Anti-Angiopoietin 1 antibody [EPR2888(N)] (ab183701) at 1/50000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lane 4 : ECV-304 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 57 kDa -
![Anti-Angiopoietin 1 antibody [EPR2888(N)] (ab183701)](https://www.abcam.com/ps/products/183/ab183701/Images/ab183701-3-benefits-of-recombinant-antibodies.png)
Anti-Angiopoietin 1 antibody [EPR2888(N)] (ab183701)
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (12)
ab183701 has been referenced in 12 publications.
- Duan YY et al. Microtubule Stabilization Promotes Microcirculation Reconstruction After Spinal Cord Injury. J Mol Neurosci 71:583-595 (2021). PubMed: 32901373
- Zhang Y et al. DPSCs treated by TGF-ß1 regulate angiogenic sprouting of three-dimensionally co-cultured HUVECs and DPSCs through VEGF-Ang-Tie2 signaling. Stem Cell Res Ther 12:281 (2021). PubMed: 33971955
- Fan X et al. Human endometrium-derived stem cell improves cardiac function after myocardial ischemic injury by enhancing angiogenesis and myocardial metabolism. Stem Cell Res Ther 12:344 (2021). PubMed: 34112245
- Yusof MFH et al. Amniotic Membrane Enhance the Effect of Vascular Endothelial Growth Factor on the Angiogenic Marker Expression of Stem Cells from Human Exfoliated Deciduous Teeth. Appl Biochem Biotechnol 191:177-190 (2020). PubMed: 32096060
- Kumar H et al. Elevated TRPV4 Levels Contribute to Endothelial Damage and Scarring in Experimental Spinal Cord Injury. J Neurosci 40:1943-1955 (2020). PubMed: 31974206
