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    angiopoietin-1-antibody-epr2888n-ab183701.pdf

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RecombinantRabMAb

Recombinant Anti-Angiopoietin 1 antibody [EPR2888(N)] (ab183701)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-Angiopoietin 1 antibody [EPR2888(N)] (ab183701)
  • Western blot - Anti-Angiopoietin 1 antibody [EPR2888(N)] (ab183701)
  • Anti-Angiopoietin 1 antibody [EPR2888(N)] (ab183701)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2888(N)] to Angiopoietin 1
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Protein
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Recombinant Human Angiopoietin 1 protein (denatured) (ab113163)
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Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (ab124801)
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10X RIPA Buffer (ab156034)

View more associated products

Overview

  • Product name

    Anti-Angiopoietin 1 antibody [EPR2888(N)]
    See all Angiopoietin 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR2888(N)] to Angiopoietin 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: U87-MG, HeLa, C6, RAW 264.7, K562, and ECV-304 cell lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2888(N)
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • Angiopoietin
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia

Associated products

  • Alternative Versions

    • Anti-Angiopoietin 1 antibody [EPR2888(N)] - BSA and Azide free (ab250679)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • K-562 whole cell lysate (ab7911)
  • Recombinant Protein

    • Recombinant Human Angiopoietin 1 protein (denatured) (ab113163)
  • Related Products

    • Recombinant Human Angiopoietin 1 protein (denatured) (ab113163)
    • Recombinant human Angiopoietin 1 protein (ab69492)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab183701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa).
Notes
WB
1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa).

Target

  • Function

    Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development.
  • Sequence similarities

    Contains 1 fibrinogen C-terminal domain.
  • Post-translational
    modifications

    Glycosylated.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession Q15389 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 284 Human
    • Entrez Gene: 11600 Mouse
    • Entrez Gene: 89807 Rat
    • Omim: 601667 Human
    • SwissProt: Q15389 Human
    • SwissProt: O08538 Mouse
    • SwissProt: O35460 Rat
    • Unigene: 369675 Human
    • Unigene: 309336 Mouse
    • Unigene: 161953 Rat
    see all
  • Alternative names

    • AGP 1 antibody
    • AGP1 antibody
    • AGPT antibody
    • ANG 1 antibody
    • ANG antibody
    • ANG-1 antibody
    • ANG1 antibody
    • Angiopoietin-1 antibody
    • Angiopoietin1 antibody
    • ANGP1_HUMAN antibody
    • ANGPT 1 antibody
    • Angpt1 antibody
    • KIAA0003 antibody
    • OTTHUMP00000227592 antibody
    • OTTHUMP00000227599 antibody
    • OTTHUMP00000227600 antibody
    see all

Images

  • Western blot - Anti-Angiopoietin 1 antibody [EPR2888(N)] (ab183701)
    Western blot - Anti-Angiopoietin 1 antibody [EPR2888(N)] (ab183701)
    All lanes : Anti-Angiopoietin 1 antibody [EPR2888(N)] (ab183701) at 1/10000 dilution (Purified)

    Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates
    Lane 2 : C6 (Rat glial tumor glial cell) whole cell lysates
    Lane 3 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
    Lane 4 : Human brain lysates
    Lane 5 : Mouse brain lysates
    Lane 6 : Rat brain lysates
    Lane 7 : Human heart lysates
    Lane 8 : Mouse heart lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 57 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?



    This antibody is unsuitable to detect tissue lysate samples.

  • Western blot - Anti-Angiopoietin 1 antibody [EPR2888(N)] (ab183701)
    Western blot - Anti-Angiopoietin 1 antibody [EPR2888(N)] (ab183701)
    All lanes : Anti-Angiopoietin 1 antibody [EPR2888(N)] (ab183701) at 1/50000 dilution

    Lane 1 : U87-MG cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : ECV-304 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 57 kDa

  • Anti-Angiopoietin 1 antibody [EPR2888(N)] (ab183701)
    Anti-Angiopoietin 1 antibody [EPR2888(N)] (ab183701)

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (12)

Publishing research using ab183701? Please let us know so that we can cite the reference in this datasheet.

ab183701 has been referenced in 12 publications.

  • Duan YY  et al. Microtubule Stabilization Promotes Microcirculation Reconstruction After Spinal Cord Injury. J Mol Neurosci 71:583-595 (2021). PubMed: 32901373
  • Zhang Y  et al. DPSCs treated by TGF-ß1 regulate angiogenic sprouting of three-dimensionally co-cultured HUVECs and DPSCs through VEGF-Ang-Tie2 signaling. Stem Cell Res Ther 12:281 (2021). PubMed: 33971955
  • Fan X  et al. Human endometrium-derived stem cell improves cardiac function after myocardial ischemic injury by enhancing angiogenesis and myocardial metabolism. Stem Cell Res Ther 12:344 (2021). PubMed: 34112245
  • Yusof MFH  et al. Amniotic Membrane Enhance the Effect of Vascular Endothelial Growth Factor on the Angiogenic Marker Expression of Stem Cells from Human Exfoliated Deciduous Teeth. Appl Biochem Biotechnol 191:177-190 (2020). PubMed: 32096060
  • Kumar H  et al. Elevated TRPV4 Levels Contribute to Endothelial Damage and Scarring in Experimental Spinal Cord Injury. J Neurosci 40:1943-1955 (2020). PubMed: 31974206
View all Publications for this product

Customer reviews and Q&As

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