RecombinantRabMAb

Recombinant Anti-Angiopoietin 1 antibody [EPR2888(N)] (ab183701)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2888(N)] to Angiopoietin 1
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Angiopoietin 1 antibody [EPR2888(N)]
    See all Angiopoietin 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR2888(N)] to Angiopoietin 1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Angiopoietin 1 aa 250-350. The exact sequence is proprietary.
    Database link: Q15389

  • Positive control

    • U87-MG, HeLa, C6, RAW 264.7, K562 and ECV-304 cell lysates. Human brain, Mouse brain, Rat brain, Human heart, and Mouse heart tissue lysates

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab183701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa).

Target

  • Function

    Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development.

  • Sequence similarities

    Contains 1 fibrinogen C-terminal domain.

  • Post-translational
    modifications

    Glycosylated.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • AGP 1 antibody
    • AGP1 antibody
    • AGPT antibody
    • ANG 1 antibody
    • ANG antibody
    • ANG-1 antibody
    • ANG1 antibody
    • Angiopoietin-1 antibody
    • Angiopoietin1 antibody
    • ANGP1_HUMAN antibody
    • ANGPT 1 antibody
    • Angpt1 antibody
    • KIAA0003 antibody
    • OTTHUMP00000227592 antibody
    • OTTHUMP00000227599 antibody
    • OTTHUMP00000227600 antibody
    see all

Images

  • Western blot - Anti-Angiopoietin 1 antibody [EPR2888(N)] (ab183701)
    Western blot - Anti-Angiopoietin 1 antibody [EPR2888(N)] (ab183701)
    All lanes : Anti-Angiopoietin 1 antibody [EPR2888(N)] (ab183701) at 1/10000 dilution (Purified)

    Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates
    Lane 2 : C6 (Rat glial tumor glial cell) whole cell lysates
    Lane 3 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
    Lane 4 : Human brain lysates
    Lane 5 : Mouse brain lysates
    Lane 6 : Rat brain lysates
    Lane 7 : Human heart lysates
    Lane 8 : Mouse heart lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 57 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?



    This antibody is unsuitable to detect tissue lysate samples.

  • Western blot - Anti-Angiopoietin 1 antibody [EPR2888(N)] (ab183701)
    Western blot - Anti-Angiopoietin 1 antibody [EPR2888(N)] (ab183701)
    All lanes : Anti-Angiopoietin 1 antibody [EPR2888(N)] (ab183701) at 1/50000 dilution

    Lane 1 : U87-MG cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : ECV-304 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 57 kDa

  • Anti-Angiopoietin 1 antibody [EPR2888(N)] (ab183701)
    Anti-Angiopoietin 1 antibody [EPR2888(N)] (ab183701)

References (5)

Publishing research using ab183701? Please let us know so that we can cite the reference in this datasheet.

ab183701 has been referenced in 5 publications.

  • Wang T  et al. Metastasis-Associated 1 (MTA1) Gene Expression Promotes Angiogenesis in Mouse Xenografts from Human Non-Small Cell Lung Cancer (NSCLC) Cells. Med Sci Monit 25:484-491 (2019). PubMed: 30651530
  • Zhu Y  et al. The TRPC5 channel regulates angiogenesis and promotes recovery from ischemic injury in mice. J Biol Chem 294:28-37 (2019). PubMed: 30413532
  • Dekker NAM  et al. Vasculotide, an angiopoietin-1 mimetic, reduces pulmonary vascular leakage and preserves microcirculatory perfusion during cardiopulmonary bypass in rats. Br J Anaesth 121:1041-1051 (2018). PubMed: 30336848
  • Han HH  et al. Angiopoietin-2 promotes ER+ breast cancer cell survival in bone marrow niche. Endocr Relat Cancer 23:609-23 (2016). WB ; Human . PubMed: 27353038
  • Gu H  et al. Angiopoietin-1 and Angiopoietin-2 Expression Imbalance Influence in Early Period After Subarachnoid Hemorrhage. Int Neurourol J 20:288-295 (2016). PubMed: 28043115

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