Overview

  • Product name
    Anti-Angiopoietin 2 antibody [EPR2891(2)]
    See all Angiopoietin 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR2891(2)] to Angiopoietin 2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Fr, WBmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Angiopoietin 2. The exact sequence is proprietary.
    Database link: O15123

  • Positive control
    • WB: TF1, HUVEC and ECV-304 cell lysates. Mouse spleen and rat kidney tissue lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR2891(2)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/500.
WB 1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function
      Can induce tyrosine phosphorylation of TIE2. Binds to TIE2 receptor and counteracts blood vessel maturation/stability mediated by angiopoietin-1. Its function may be context-dependent. In the absence of angiogenic inducers, such as VEGF, ANG2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
    • Sequence similarities
      Contains 1 fibrinogen C-terminal domain.
    • Domain
      The Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • AGPT 2 antibody
      • Agpt2 antibody
      • ANG 2 antibody
      • ANG-2 antibody
      • ANG2 antibody
      • Angiopoietin 2a antibody
      • Angiopoietin 2B antibody
      • Angiopoietin-2 antibody
      • Angiopoietin2 antibody
      • ANGP2_HUMAN antibody
      • ANGPT 2 antibody
      • Angpt2 antibody
      • Tie2 ligand antibody
      see all

    Images

    • All lanes : Anti-Angiopoietin 2 antibody [EPR2891(2)] (ab155106) at 1/5000 dilution (purified)

      Lane 1 : Mouse spleen tissue lysate
      Lane 2 : Rat kidney tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 57 kDa
      Observed band size: 55 kDa
      why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM /TBST.

    • All lanes : Anti-Angiopoietin 2 antibody [EPR2891(2)] (ab155106) at 1/5000 dilution (purified)

      Lane 1 : TF-1 whole cell lysate
      Lane 2 : HUVEC whole cell lysate
      Lane 3 : ECV-304 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 57 kDa
      Observed band size: 55 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM /TBST.

    • All lanes : Anti-Angiopoietin 2 antibody [EPR2891(2)] (ab155106) at 1/1000 dilution (unpurified)

      Lane 1 : TF1 cell lysate
      Lane 2 : HUVEC cell lysate
      Lane 3 : ECV-304 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 57 kDa

    • Unpurified ab155106 staining Angiopoietin 2 in Mouse fetal lung tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with PBST for 1 hour and blocked with 1.5% serum for 20 minutes at 25°C. Samples were incubated with primary antibody (1/500 in PBST) for 12 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

      See Abreview

    References

    This product has been referenced in:
    • Chen Y  et al. Angiopoietin-2 (Ang-2) is a useful serum tumor marker for liver cancer in the Chinese population. Clin Chim Acta 478:18-27 (2018). WB, IHC, ICC/IF ; Human . Read more (PubMed: 29253494) »
    • Dong Z  et al. Ang-2 promotes lung cancer metastasis by increasing epithelial-mesenchymal transition. Oncotarget 9:12705-12717 (2018). Read more (PubMed: 29560103) »
    See all 8 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (liver)
    Specification
    liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

    Abcam user community

    Verified customer

    Submitted Dec 25 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 1.5% · Temperature: 25°C
    Sample
    Mouse Tissue sections (fetal lung)
    Specification
    fetal lung
    Permeabilization
    Yes - PBST for 1 hour
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Jul 05 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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