Overview

  • Product name
    Anti-Angiopoietin 2 antibody [MM0020-1F29]
    See all Angiopoietin 2 primary antibodies
  • Description
    Mouse monoclonal [MM0020-1F29] to Angiopoietin 2
  • Host species
    Mouse
  • Specificity
    This antibody recognizes Angiopoietin 2 in western blot without cross reactivity with Angiopoietin 1.
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant purified full length protein (Human).

  • Positive control
    • Human endothelial cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    The IgG fraction of the tissue culture supernatant was purified and then lyophilized from 0.2 µm filtered PBS solution.
  • Clonality
    Monoclonal
  • Clone number
    MM0020-1F29
  • Isotype
    IgG2
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab56301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/200.
WB 1/400 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 57 kDa).

Target

  • Function
    Can induce tyrosine phosphorylation of TIE2. Binds to TIE2 receptor and counteracts blood vessel maturation/stability mediated by angiopoietin-1. Its function may be context-dependent. In the absence of angiogenic inducers, such as VEGF, ANG2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
  • Sequence similarities
    Contains 1 fibrinogen C-terminal domain.
  • Domain
    The Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • AGPT 2 antibody
    • Agpt2 antibody
    • ANG 2 antibody
    • ANG-2 antibody
    • ANG2 antibody
    • Angiopoietin 2a antibody
    • Angiopoietin 2B antibody
    • Angiopoietin-2 antibody
    • Angiopoietin2 antibody
    • ANGP2_HUMAN antibody
    • ANGPT 2 antibody
    • Angpt2 antibody
    • Tie2 ligand antibody
    see all

Images

  • ab56301 at 1/200 dilution, staining Angiopoietin 2 in human placental tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).

  • Anti-Angiopoietin 2 antibody [MM0020-1F29] (ab56301) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 57 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 47 kDa, 65 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes


    The band observed at 52 kDa could potentially be a cleaved form of Angiopoietin 2 due to the presence of an 18 amino acid signal peptide.

References

ab56301 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Question
Answer

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab56301. The item will be shipped on Monday for Tuesday delivery.
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.

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Answer

Thank you for your reply.
I have aligned the human angiopoietin 1 and 2 proteins and the overall sequence homology is only 61%.
I would recommend ab64015, since the immunogen does not align well with Ang1 (63% homology)
https://www.abcam.com/Angiopoietin-2-antibody-ab64015.html
or ab56301 (https://www.abcam.com/Angiopoietin-2-antibody-MM0020-1F29-ab56301.html) as this antibody recognizes Angiopoietin 2 in western blot without cross reactivity with Angiopoietin 1.
Please let me know which of the 2 antibodies you would prefer and I will arrange the shipment for you. The order I have is the correct one. Thank you for the additional order information.
Also, if the shipping address has changed in the meantime (since August), please let me know the new address.
I look forward to hear back from you.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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