Anti-Angiopoietin 2/ANG2 antibody (ab153934)
- Datasheet
- References (5)
- Protocols
Overview
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Product name
Anti-Angiopoietin 2/ANG2 antibody
See all Angiopoietin 2/ANG2 primary antibodies -
Description
Rabbit polyclonal to Angiopoietin 2/ANG2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Dog, Pig
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Immunogen
Recombinant fragment corresponding to Human Angiopoietin 2/ANG2 aa 1-308.
Database link: O15123 -
Positive control
- Human colon carcinoma tissue; A549 cells; HepG2 whole cell lysate (ab7900).
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General notes
This product was previously labelled as Angiopoietin 2
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab153934 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 57 kDa. | |
| IHC-P | 1/100 - 1/1000. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Can induce tyrosine phosphorylation of TIE2. Binds to TIE2 receptor and counteracts blood vessel maturation/stability mediated by angiopoietin-1. Its function may be context-dependent. In the absence of angiogenic inducers, such as VEGF, ANG2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. -
Sequence similarities
Contains 1 fibrinogen C-terminal domain. -
Domain
The Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 607616 Dog
- Entrez Gene: 285 Human
- Entrez Gene: 396730 Pig
- Entrez Gene: 89805 Rat
- Omim: 601922 Human
- SwissProt: A0A8J8 Dog
- SwissProt: O15123 Human
- SwissProt: Q9BDY7 Pig
see all -
Alternative names
- AGPT 2 antibody
- Agpt2 antibody
- ANG 2 antibody
see all
Images
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Western blot - Anti-Angiopoietin 2/ANG2 antibody (ab153934)Anti-Angiopoietin 2/ANG2 antibody (ab153934) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 57 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiopoietin 2/ANG2 antibody (ab153934)Immunohistochemical analysis of paraffin-embedded Human colon carcinoma, labeling Angiopoietin 2/ANG2 with ab153934 at 1/500 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin 2/ANG2 antibody (ab153934)Immunofluorescent analysis of methanol-fixed A549 cells, labeling Angiopoietin 2/ANG2 with ab153934 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
Protocols
References
This product has been referenced in:
- Pianta S et al. A Short Bout of Exercise Prior to Stroke Improves Functional Outcomes by Enhancing Angiogenesis. Neuromolecular Med N/A:N/A (2019). Read more (PubMed: 30941660) »
- Magkouta S et al. Targeting Tie-2/angiopoietin axis in experimental mesothelioma confers differential responses and raises predictive implications. Oncotarget 9:21783-21796 (2018). Read more (PubMed: 29774102) »
