Key features and details
- Rabbit polyclonal to Angiopoietin 2/ANG2
- Suitable for: WB
- Reacts with: Mouse, Human, Pig
- Isotype: IgG
Product nameAnti-Angiopoietin 2/ANG2 antibody
See all Angiopoietin 2/ANG2 primary antibodies
DescriptionRabbit polyclonal to Angiopoietin 2/ANG2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human, Pig
Predicted to work with: Rat, Cow, Dog
- WB: Recombinant pig Angiopoietin 2/ANG2 protein; Pig and mouse brain lysates; K562 cell lysate.
This product was previously labelled as Angiopoietin 2
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab231251 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 57 kDa.|
FunctionCan induce tyrosine phosphorylation of TIE2. Binds to TIE2 receptor and counteracts blood vessel maturation/stability mediated by angiopoietin-1. Its function may be context-dependent. In the absence of angiogenic inducers, such as VEGF, ANG2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
Sequence similaritiesContains 1 fibrinogen C-terminal domain.
DomainThe Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor.
- Information by UniProt
- AGPT 2 antibody
- Agpt2 antibody
- ANG 2 antibody
All lanes : Anti-Angiopoietin 2/ANG2 antibody (ab231251) at 3 µg/ml
Lane 1 : Pig brain lysate
Lane 2 : Mouse brain lysate
Lane 3 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
All lanes : HRP-linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 57 kDa
Anti-Angiopoietin 2/ANG2 antibody (ab231251) at 3 µg/ml + Recombinant pig Angiopoietin 2/ANG2 protein
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 57 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231251 has not yet been referenced specifically in any publications.