Recombinant Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] - BSA and Azide free (ab285368)
![Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] - BSA and Azide free (ab285368)](https://www.abcam.com/ps/products/285/ab285368/Images/ab285368-476774-anti-angiopoietin-2ang2-antibody-epr2416173-western-blot-lysate2.jpg "Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] - BSA and Azide free (ab285368)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24161-73] to Angiopoietin 2/ANG2 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] - BSA and Azide free
See all Angiopoietin 2/ANG2 primary antibodies -
Description
Rabbit monoclonal [EPR24161-73] to Angiopoietin 2/ANG2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt (Intra),ICC,IHC-Fr,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HUVEC, TF-1, TF-1 treated with PNGase F whole, Human placenta, Mouse placenta, Mouse ovary, Rat ovary and Rat placenta.
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General notes
ab285368 is the carrier-free version of ab259823.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24161-73 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab285368 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 56 kDa.
|
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 56 kDa. |
Target
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Function
Can induce tyrosine phosphorylation of TIE2. Binds to TIE2 receptor and counteracts blood vessel maturation/stability mediated by angiopoietin-1. Its function may be context-dependent. In the absence of angiogenic inducers, such as VEGF, ANG2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. -
Sequence similarities
Contains 1 fibrinogen C-terminal domain. -
Domain
The Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 285 Human
- Entrez Gene: 11601 Mouse
- Entrez Gene: 89805 Rat
- Omim: 601922 Human
- SwissProt: O15123 Human
- SwissProt: O35608 Mouse
- SwissProt: O35462 Rat
- Unigene: 583870 Human
see all -
Alternative names
- AGPT 2 antibody
- Agpt2 antibody
- ANG 2 antibody
see all
Images
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Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] - BSA and Azide free (ab285368)All lanes : Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] (ab259823) at 1/1000 dilution
Lane 1 : Rat ovary tissue lysate
Lane 2 : Rat placenta tissue lysate
Lane 3 : Rat skeletal muscle tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lane 1 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Lanes 2-3 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 60-75 kDa why is the actual band size different from the predicted?This data was developed using ab259823, the same antibody clone in a different buffer formulation.
The molecular weight observed is consistent with what has been described in the literature (PMID:29118335, PMID:32908006)
Positive tissue: placenta, ovary (PMID:9204896)
Negative control: skeletal muscle (PMID:9204896).
Exposure time: 114 seconds
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![Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] - BSA and Azide free (ab285368)](https://www.abcam.com/ps/products/285/ab285368/Images/ab285368-476773-anti-angiopoietin-2ang2-antibody-epr2416173-western-blot-lysate1.jpg)
Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] - BSA and Azide free (ab285368)All lanes : Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] (ab259823) at 1/1000 dilution
Lane 1 : Mouse placenta tissue lysate
Lane 2 : Mouse ovary tissue lysate
Lane 3 : Mouse pancreas tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 60-75 kDa why is the actual band size different from the predicted?This data was developed using ab259823, the same antibody clone in a different buffer formulation.
The molecular weight observed is consistent with what has been described in the literature (PMID:29118335, PMID:32908006)
Positive tissue: placenta, ovary (PMID:9204896)
Exposure time: 3 minutes
-
![Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] - BSA and Azide free (ab285368)](https://www.abcam.com/ps/products/285/ab285368/Images/ab285368-476772-anti-angiopoietin-2ang2-antibody-epr2416173-western-blot-lysate.jpg)
Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] - BSA and Azide free (ab285368)Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] (ab259823) at 1/1000 dilution + Human placenta tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 56 kDa
Observed band size: 60-75 kDa why is the actual band size different from the predicted?This data was developed using ab259823, the same antibody clone in a different buffer formulation.
The molecular weight observed is consistent with what has been described in the literature (PMID:29118335, PMID:32908006)
Positive tissue: placenta (PMID:9204896)
Exposure time: 48 seconds
-
![Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] - BSA and Azide free (ab285368)](https://www.abcam.com/ps/products/285/ab285368/Images/ab285368-476771-anti-angiopoietin-2ang2-antibody-epr2416173-western-blot-vein.jpg)
Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] - BSA and Azide free (ab285368)All lanes : Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] (ab259823) at 1/1000 dilution
Lane 1 : HUVEC (human umbilical vein endothelial cell) whole cell lysate
Lane 2 : HEK-293 (human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : TF-1 (human erythroleukemia erythroblast) whole cell lysate
Lane 4 : TF-1 treated with PNGase F whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lane 1 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Lanes 2-4 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 60-75 kDa why is the actual band size different from the predicted?This data was developed using ab259823, the same antibody clone in a different buffer formulation.
The molecular weight observed is consistent with what has been described in the literature (PMID:29118335, PMID:32908006)
Positive cell line: HUVEC (PMID:25857554)
Negative control: HEK-293 (PMID:32908006)
Lanes 1-2: 3 minutes; Lanes 3-4: 81 seconds.
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![Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] - BSA and Azide free (ab285368)](https://www.abcam.com/ps/products/285/ab285368/Images/ab285368-476953-Benefits-of-Recombinant-antibodies.jpg)
Anti-Angiopoietin 2/ANG2 antibody [EPR24161-73] - BSA and Azide free (ab285368)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab285368 has not yet been referenced specifically in any publications.
