Anti-Angiopoietin 2/ANG2 antibody [MM0020-1F29] (ab56301)
Key features and details
- Mouse monoclonal [MM0020-1F29] to Angiopoietin 2/ANG2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG2
Overview
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Product name
Anti-Angiopoietin 2/ANG2 antibody [MM0020-1F29]
See all Angiopoietin 2/ANG2 primary antibodies -
Description
Mouse monoclonal [MM0020-1F29] to Angiopoietin 2/ANG2 -
Host species
Mouse -
Specificity
This antibody recognizes Angiopoietin 2/ANG2 in western blot without cross reactivity with Angiopoietin 1.
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Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human Angiopoietin 2/ANG2.
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Positive control
- Human endothelial cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
The IgG fraction of the tissue culture supernatant was purified and then lyophilized from 0.2 µm filtered PBS solution. -
Clonality
Monoclonal -
Clone number
MM0020-1F29 -
Isotype
IgG2 -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab56301 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/200.
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WB |
1/400 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 57 kDa).
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Notes |
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IHC-P
1/100 - 1/200. |
WB
1/400 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 57 kDa). |
Target
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Function
Can induce tyrosine phosphorylation of TIE2. Binds to TIE2 receptor and counteracts blood vessel maturation/stability mediated by angiopoietin-1. Its function may be context-dependent. In the absence of angiogenic inducers, such as VEGF, ANG2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. -
Sequence similarities
Contains 1 fibrinogen C-terminal domain. -
Domain
The Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 285 Human
- Omim: 601922 Human
- SwissProt: O15123 Human
- Unigene: 583870 Human
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Alternative names
- AGPT 2 antibody
- Agpt2 antibody
- ANG 2 antibody
see all
Images
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ab56301 at 1/200 dilution, staining Angiopoietin 2/ANG2 in human placental tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).
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Anti-Angiopoietin 2/ANG2 antibody [MM0020-1F29] (ab56301) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Additional bands at: 47 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutesThe band observed at 52 kDa could potentially be a cleaved form of Angiopoietin 2/ANG2 due to the presence of an 18 amino acid signal peptide.
Protocols
Datasheets and documents
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Datasheet download
References (7)
ab56301 has been referenced in 7 publications.
- Harbuzariu A et al. Neuregulin-1/ErbB4 signaling modulates Plasmodium falciparum HRP2-induced damage to brain cortical organoids. iScience 25:104407 (2022). PubMed: 35663028
- Diao H et al. miR-135a-5p inhibits tumor invasion by targeting ANGPT2 in gallbladder cancer. Mol Med Rep 24:N/A (2021). PubMed: 34036386
- Kim YJ et al. Suppression of breast cancer progression by FBXL16 via oxygen-independent regulation of HIF1α stability. Cell Rep 37:109996 (2021). PubMed: 34818544
- Guo W et al. Analysis of vascular-associated factors and the prognosis of poorly differentiated hypopharyngeal carcinoma. Oncol Lett 20:271 (2020). PubMed: 32989405
- Zhou CF et al. miR-205-5p inhibits human endometriosis progression by targeting ANGPT2 in endometrial stromal cells. Stem Cell Res Ther 10:287 (2019). PubMed: 31547870
- Lee YJ et al. Hyperhomocysteinemia Causes Chorioretinal Angiogenesis with Placental Growth Factor Upregulation. Sci Rep 8:15755 (2018). PubMed: 30361676
- Lee YJ et al. Homocysteine Facilitates Prominent Polygonal Angiogenetic Networks of a Choroidal Capillary Sprouting Model. Invest Ophthalmol Vis Sci 58:4332–4343 (2017). PubMed: 28806447