Key features and details
- Mouse monoclonal [MM0020-1F29] to Angiopoietin 2/ANG2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG2
Product nameAnti-Angiopoietin 2/ANG2 antibody [MM0020-1F29]
See all Angiopoietin 2/ANG2 primary antibodies
DescriptionMouse monoclonal [MM0020-1F29] to Angiopoietin 2/ANG2
This antibody recognizes Angiopoietin 2/ANG2 in western blot without cross reactivity with Angiopoietin 1.
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human Angiopoietin 2/ANG2.
- Human endothelial cell lysate.
This product was previously labelled as Angiopoietin 2
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein G purified
Purification notesThe IgG fraction of the tissue culture supernatant was purified and then lyophilized from 0.2 µm filtered PBS solution.
Our Abpromise guarantee covers the use of ab56301 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/200.|
|WB||1/400 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 57 kDa).|
FunctionCan induce tyrosine phosphorylation of TIE2. Binds to TIE2 receptor and counteracts blood vessel maturation/stability mediated by angiopoietin-1. Its function may be context-dependent. In the absence of angiogenic inducers, such as VEGF, ANG2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
Sequence similaritiesContains 1 fibrinogen C-terminal domain.
DomainThe Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor.
- Information by UniProt
- AGPT 2 antibody
- Agpt2 antibody
- ANG 2 antibody
ab56301 at 1/200 dilution, staining Angiopoietin 2/ANG2 in human placental tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).
Anti-Angiopoietin 2/ANG2 antibody [MM0020-1F29] (ab56301) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Additional bands at: 47 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
The band observed at 52 kDa could potentially be a cleaved form of Angiopoietin 2/ANG2 due to the presence of an 18 amino acid signal peptide.
ab56301 has been referenced in 3 publications.
- Zhou CF et al. miR-205-5p inhibits human endometriosis progression by targeting ANGPT2 in endometrial stromal cells. Stem Cell Res Ther 10:287 (2019). PubMed: 31547870
- Lee YJ et al. Hyperhomocysteinemia Causes Chorioretinal Angiogenesis with Placental Growth Factor Upregulation. Sci Rep 8:15755 (2018). PubMed: 30361676
- Lee YJ et al. Homocysteine Facilitates Prominent Polygonal Angiogenetic Networks of a Choroidal Capillary Sprouting Model. Invest Ophthalmol Vis Sci 58:4332–4343 (2017). PubMed: 28806447