Overview

  • Product name

    Anti-Angiopoietin-like 4/ANGPTL4 antibody
    See all Angiopoietin-like 4/ANGPTL4 primary antibodies
  • Description

    Rabbit polyclonal to Angiopoietin-like 4/ANGPTL4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human Angiopoietin-like 4/ANGPTL4 (internal sequence).
    Database link: Q9BY76

  • Positive control

    • Skeletal Muscle (Mouse) Whole Cell Lysate (ab3869) can be used as a positive control in WB. Mouse skeletal muscle extract; Rat lung and Human esophageal cancer tissues; A549 cells.
  • General notes

     This product was previously labelled as Angiopoietin-like 4

     

Properties

Applications

Our Abpromise guarantee covers the use of ab196746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/200.
IHC-P 1/50 - 1/200.
WB 1/500 - 1/2000. Predicted molecular weight: 45 kDa.

Target

  • Function

    Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorgenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation.
  • Tissue specificity

    Expressed at high levels in the placenta, heart, liver, muscle, pancreas and lung but expressed poorly in the brain and kidney.
  • Sequence similarities

    Contains 1 fibrinogen C-terminal domain.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Secreted. Secreted > extracellular space > extracellular matrix. The unprocessed form interacts with the extracellular matrix. This may constitute a dynamic reservoir, a regulatory mechanism of the bioavailability of ANGPTL4.
  • Information by UniProt
  • Database links

  • Alternative names

    • Angiopoietin like 4 antibody
    • Angiopoietin related protein 4 antibody
    • Angiopoietin-like protein 4 antibody
    • Angiopoietin-related protein 4 antibody
    • ANGL4_HUMAN antibody
    • ANGPT L2 antibody
    • ANGPT L4 antibody
    • ANGPTL2 antibody
    • Angptl4 antibody
    • ARP4 antibody
    • Fasting induced adipose factor antibody
    • FIAF antibody
    • HARP antibody
    • Hepatic angiopoietin related protein antibody
    • Hepatic fibrinogen/angiopoietin related protein antibody
    • Hepatic fibrinogen/angiopoietin-related protein antibody
    • HFARP antibody
    • NL2 antibody
    • Peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin related protein antibody
    • PGAR antibody
    • pp1158 antibody
    • PPARG angiopoietin related protein antibody
    • PSEC0166 antibody
    • TGQTL antibody
    • UNQ171 antibody
    • Weakly similar to angiopoietin 1 [H.sapiens] antibody
    see all

Images

  • Anti-Angiopoietin-like 4/ANGPTL4 antibody (ab196746) at 1/500 dilution + Mouse skeletal muscle extract

    Predicted band size: 45 kDa

  • Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Angiopoietin-like 4/ANGPTL4 with ab196746 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissue labeling Angiopoietin-like 4/ANGPTL4 with ab196746 at 1/100 dilution.

  • Immunofluorescent analysis of A549 cells labeling Angiopoietin-like 4/ANGPTL4 with ab196746 at 1/50 dilution. Blue: DAPI for nuclear staining.

References

This product has been referenced in:

  • Zou T  et al. Sema4D/PlexinB1 promotes endothelial differentiation of dental pulp stem cells via activation of AKT and ERK1/2 signaling. J Cell Biochem N/A:N/A (2019). Read more (PubMed: 30937968) »
  • Zhao L  et al. PPAR signaling pathway in the first trimester placenta from in vitro fertilization and embryo transfer. Biomed Pharmacother 118:109251 (2019). Read more (PubMed: 31351426) »
See all 5 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Immunocytochemistry
Sample
Mouse Cultured Cells (Cardiomyocytes)
Permeabilization
Yes - Triton x-100, 0.03%
Specification
Cardiomyocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde

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Verified customer

Submitted Feb 27 2018

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