Anti-Angiopoietin-like 4/ANGPTL4 antibody (ab2920)

Reviews (1)Q&A (4)References (5)
Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody (ab2920)

    Key features and details

    • Rabbit polyclonal to Angiopoietin-like 4/ANGPTL4
    • Suitable for: WB
    • Reacts with: Rat
    • Isotype: IgG

    Overview

    • Product name

      Anti-Angiopoietin-like 4/ANGPTL4 antibody
      See all Angiopoietin-like 4/ANGPTL4 primary antibodies

    • Description

      Rabbit polyclonal to Angiopoietin-like 4/ANGPTL4

    • Host species

      Rabbit

    • Specificity

      Detects fasting-induced adipose factor (FIAF) from rat adipose tissue.

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Rat

    • Immunogen

      Synthetic peptide corresponding to Mouse Angiopoietin-like 4/ANGPTL4 aa 80-94 (N terminal).
      Sequence:

      CQGPKGKDAPFKDSE


      (Peptide available as ab4936)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • Positive control

      • Rat adipose tissue extracts.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab2920 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (1)
    Use a concentration of 2 µg/ml.

    By Western blot, this antibody detects an ~50 kDa protein representing FIAF from rat adipose tissue extracts.
    Notes
    WB
    Use a concentration of 2 µg/ml.

    By Western blot, this antibody detects an ~50 kDa protein representing FIAF from rat adipose tissue extracts.

    Target

    • Function

      Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorgenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation.

    • Tissue specificity

      Expressed at high levels in the placenta, heart, liver, muscle, pancreas and lung but expressed poorly in the brain and kidney.

    • Sequence similarities

      Contains 1 fibrinogen C-terminal domain.

    • Post-translational
      modifications

      N-glycosylated.

    • Cellular localization

      Secreted. Secreted > extracellular space > extracellular matrix. The unprocessed form interacts with the extracellular matrix. This may constitute a dynamic reservoir, a regulatory mechanism of the bioavailability of ANGPTL4.
    • Information by UniProt

    • Database links

      • Alternative names

        • Angiopoietin like 4 antibody
        • Angiopoietin related protein 4 antibody
        • Angiopoietin-like protein 4 antibody
        • Angiopoietin-related protein 4 antibody
        • ANGL4_HUMAN antibody
        • ANGPT L2 antibody
        • ANGPT L4 antibody
        • ANGPTL2 antibody
        • Angptl4 antibody
        • ARP4 antibody
        • Fasting induced adipose factor antibody
        • FIAF antibody
        • HARP antibody
        • Hepatic angiopoietin related protein antibody
        • Hepatic fibrinogen/angiopoietin related protein antibody
        • Hepatic fibrinogen/angiopoietin-related protein antibody
        • HFARP antibody
        • NL2 antibody
        • Peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin related protein antibody
        • PGAR antibody
        • pp1158 antibody
        • PPARG angiopoietin related protein antibody
        • PSEC0166 antibody
        • TGQTL antibody
        • UNQ171 antibody
        • Weakly similar to angiopoietin 1 [H.sapiens] antibody
        see all

      Images

      • Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody (ab2920)
        Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody (ab2920)
        Western blot on FIAF in rat adipose tissue extract using ab2920. Western blot on FIAF in rat adipose tissue extract using ab2920.

      References (5)

      Publishing research using ab2920? Please let us know so that we can cite the reference in this datasheet.

      ab2920 has been referenced in 5 publications.

      • Zhou S  et al. Adipocytes induce the resistance of ovarian cancer to carboplatin through ANGPTL4. Oncol Rep 44:927-938 (2020). PubMed: 32705217
      • Plaza A  et al. Cholecystokinin is involved in triglyceride fatty acid uptake by rat adipose tissue. J Endocrinol 236:137-150 (2018). PubMed: 29339381
      • Rodrigues T  et al. Methylglyoxal-induced glycation changes adipose tissue vascular architecture, flow and expansion, leading to insulin resistance. Sci Rep 7:1698 (2017). WB ; Rat . PubMed: 28490763
      • Villa L  et al. Effects and mechanisms of angiotensin II receptor blockade with telmisartan in a normotensive model of mesangioproliferative nephritis. Nephrol Dial Transplant : (2011). PubMed: 21414972
      • Koliwad SK  et al. Angiopoietin-like 4 (ANGPTL4, fasting-induced adipose factor) is a direct glucocorticoid receptor target and participates in glucocorticoid-regulated triglyceride metabolism. J Biol Chem 284:25593-601 (2009). WB ; Mouse . PubMed: 19628874

      Customer reviews and Q&As

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      1-5 of 5 Abreviews or Q&A

      Western blot abreview for Anti-Angiopoietin-like 4/ANGPTL4 antibody

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - other (white fat tissue)
      Gel Running Conditions
      Non-reduced Denaturing (12.5%)
      Loading amount
      30 µg
      Specification
      white fat tissue
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
      Read More

      Abcam user community

      Verified customer

      Submitted Jan 22 2014

      Question

      Hi, I ordered the antibody (anti-angiopoitin-like 4, ab2920) this week, today I checked the expression of angiopoitin-like 4 in the lysate of 3T3-L1 adipocytes, and found that Western result showed many specific bands, the concentration of the antibodyIused in the experiment is 2ug/ml, I think this antibody is not good. Can you refund? Thanks for you consideration.

      Read More
      Answer

      Thank you fro bringing this to our attention. Are you seeing a band at 50 kDa, and if so, is it more intense than the other bands? Where do the other bands appear in the blot? If you can attach an image, the may help me understand what is going on.

      It is possible the antibody is defective but we ask our customer to provide a few details of their protocol, in the hope that we can suggest a modification that will improve the result. If not, I will send a refund.

      Can you please tell me how the samples are prepared, in particular, whether or not you are using protease inhibitors? Are the samples reduced and denatured?

      How is the membrane blocked before incubation with the antibody? And finally, are you confident that the secondary antibody is effective and not reacting with proteins in the sample?

      I look forward to your reply.

      Read More

      Question

      May I ask about expiry date of mentioned below antibodies? I couldn't find such info on your website (only storage instruction) and I'm not sure how long can I store them without the loss of their quality. Cat.no. Ab23841 Ab54481 Ab6276 Ab9484 Ab2920 Ab56023 Ab45966 Ab95453 Ab18199 Ab2911 Ab61249

      Read More
      Answer

      Thank you for your inquiry. We do not assign strict expiration dates for our products, however we follow general guidelines: Antibodies stored at 4C may be viable up to 1 year, those stored at -20C may be viable for up to 5 years, and those stored at -80C may be viable for up to 10 years. I hope this information helps. Please contact us with any other questions.

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      Question

      BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM Multiple bands I have problem to identify the right band . your antibody detects two possibly relevant bands - one week around 50 kDa and one quite strong around 30 kDa. In your datasheet 50 kDa is predicted as appearent protein size but in literature (i.e. Koster et al. Endocrinology 146: 4943 - 4950) Angptl-4 is reffered as 35 kDa protein. What band is the right band? SAMPLE Rat adipose tissue extract PRIMARY ANTIBODY ab2920 DETECTION METHOD ECL ANTIBODY STORAGE CONDITIONS -20oC SAMPLE PREPARATION RIPA buffer, Sigma protease inhibitor coctail AMOUNT OF PROTEIN LOADED 80 ug/lane ELECTROPHORESIS/GEL CONDITIONS 10% reducing gel TRANSFER AND BLOCKING CONDITIONS Glycin-Tris/Hcl buffer, 2 H 90V 5% non-fat dry milk in TBST SECONDARY ANTIBODY antirabbit Ig - HRP. cell signalling HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 5 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes

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      Answer

      Thank you for your enquiry. The predicted KDa that this target should run at (Swiss Prot) is 46KDa, there is no indication of an alternate isoform of this target that I have been able to source. The paper cited below appears to indicate that the full length target observed usually as a single band at 45KDa is the unprocessed form; however proteolytic cleavage is also reported in this paper. Where this occurs the author suggests that a band of 25-30KDa may be observed. http://www.jbc.org/cgi/reprint/M403058200v1.pdf I am unsure as to the conditions that dictate the cleavage for this target; however it does sound like you may be detecting both forms. Since the rabbit is a pab (CQGPKGKDAPFKDSE) and will detect multiple epitopes it is possible that both forms may be detected. Should you have any further questions then please do not hesitate to get back in touch with me.

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      Question

      ANTIBODY CODE ab2920 BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM Target protein is not precipitated SAMPLE 3T3-L1 cell extract ANTIBODY STORAGE CONDITIONS 4 degree HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 2 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes

      Read More
      Answer

      Thank you for your enquiry and patience. To our knowledge, this antibody has only been tested for application in Western blotting, and so we don't have any information about how ab2920 works in IP. Have you done a positive control? I would suggest running a Western with rat adipose tissue extract to ensure that the antibody is functioning properly. Also, when did you purchase this antibody? What is the lot number (located on the vial)? On the datasheet it is recommended to store this antibody at +4°C short term and aliquot and store at -20°C or -80° for longer term storage.

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