Anti-Angiopoietin-like 4/ANGPTL4 antibody (ab2920)
Key features and details
- Rabbit polyclonal to Angiopoietin-like 4/ANGPTL4
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-Angiopoietin-like 4/ANGPTL4 antibody
See all Angiopoietin-like 4/ANGPTL4 primary antibodies -
Description
Rabbit polyclonal to Angiopoietin-like 4/ANGPTL4 -
Host species
Rabbit -
Specificity
Detects fasting-induced adipose factor (FIAF) from rat adipose tissue. -
Tested Applications & Species
See all applications and species dataApplication Species WB Rat
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Immunogen
Synthetic peptide corresponding to Mouse Angiopoietin-like 4/ANGPTL4 aa 80-94 (N terminal).
Sequence:CQGPKGKDAPFKDSE
(Peptide available asab4936) -
Positive control
- Rat adipose tissue extracts.
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General notes
This product was previously labelled as Angiopoietin-like 4
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
Adipose tissue is an energy reserve in animals, and in nondomestic species it is strictly regulated. Adipose cells produce and secrete numerous physiologically important proteins that help regulate whole body metabolism, such as leptin and Acrp30. Fasting causes significant changes in nutrient metabolism and many of these changes are controlled by transcription factors that regulate rate-limiting enzymes. An important transcription factor that mediates metabolic processes induced by fasting is peroxisome proliferator-activated receptor alpha (PPAR alpha). PPAR alpha has recently been reported to target a novel gene encoding the secreted protein FIAF (fasting-induced adipose factor). FIAF is expressed predominantly in adipose tissue and is strongly upregulated in white adipose tissue and the liver under fasting conditions. Initial studies suggest that FIAF represents a novel endocrine signal helping to regulate metabolism, especially under fasting conditions. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2920 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use a concentration of 2 µg/ml.
By Western blot, this antibody detects an ~50 kDa protein representing FIAF from rat adipose tissue extracts. |
| Notes |
|---|
|
WB
Use a concentration of 2 µg/ml. By Western blot, this antibody detects an ~50 kDa protein representing FIAF from rat adipose tissue extracts. |
Target
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Function
Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorgenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation. -
Tissue specificity
Expressed at high levels in the placenta, heart, liver, muscle, pancreas and lung but expressed poorly in the brain and kidney. -
Sequence similarities
Contains 1 fibrinogen C-terminal domain. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Secreted. Secreted > extracellular space > extracellular matrix. The unprocessed form interacts with the extracellular matrix. This may constitute a dynamic reservoir, a regulatory mechanism of the bioavailability of ANGPTL4. - Information by UniProt
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Database links
- Entrez Gene: 362850 Rat
- SwissProt: Q6TMA8 Rat
- Unigene: 119611 Rat
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Alternative names
- Angiopoietin like 4 antibody
- Angiopoietin related protein 4 antibody
- Angiopoietin-like protein 4 antibody
see all
Images
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Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody (ab2920)Western blot on FIAF in rat adipose tissue extract using ab2920. Western blot on FIAF in rat adipose tissue extract using ab2920.
Datasheets and documents
References (5)
ab2920 has been referenced in 5 publications.
- Zhou S et al. Adipocytes induce the resistance of ovarian cancer to carboplatin through ANGPTL4. Oncol Rep 44:927-938 (2020). PubMed: 32705217
- Plaza A et al. Cholecystokinin is involved in triglyceride fatty acid uptake by rat adipose tissue. J Endocrinol 236:137-150 (2018). PubMed: 29339381
- Rodrigues T et al. Methylglyoxal-induced glycation changes adipose tissue vascular architecture, flow and expansion, leading to insulin resistance. Sci Rep 7:1698 (2017). WB ; Rat . PubMed: 28490763
- Villa L et al. Effects and mechanisms of angiotensin II receptor blockade with telmisartan in a normotensive model of mesangioproliferative nephritis. Nephrol Dial Transplant : (2011). PubMed: 21414972
- Koliwad SK et al. Angiopoietin-like 4 (ANGPTL4, fasting-induced adipose factor) is a direct glucocorticoid receptor target and participates in glucocorticoid-regulated triglyceride metabolism. J Biol Chem 284:25593-601 (2009). WB ; Mouse . PubMed: 19628874
