Recombinant
RabMAb

Recombinant Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420)

Overview

  • Product name

    Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873]
    See all Angiopoietin-like 4/ANGPTL4 primary antibodies
  • Description

    Rabbit monoclonal [EPR19873] to Angiopoietin-like 4/ANGPTL4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Angiopoietin-like 4/ANGPTL4 aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9BY76-1

  • Positive control

    • WB: A549, SW480, THP-1 and HT-29 whole cell lysates; human kidney and fetal lung lysates. ICC/IF: SW480 and HT-29 cells. Flow Cyt: HT-29 cells.
  • General notes

     This product was previously labelled as Angiopoietin-like 4

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab206420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 50, 35 kDa (predicted molecular weight: 45 kDa).
ICC/IF 1/100.
Flow Cyt 1/700.

Target

  • Function

    Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorgenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation.
  • Tissue specificity

    Expressed at high levels in the placenta, heart, liver, muscle, pancreas and lung but expressed poorly in the brain and kidney.
  • Sequence similarities

    Contains 1 fibrinogen C-terminal domain.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Secreted. Secreted > extracellular space > extracellular matrix. The unprocessed form interacts with the extracellular matrix. This may constitute a dynamic reservoir, a regulatory mechanism of the bioavailability of ANGPTL4.
  • Information by UniProt
  • Database links

  • Alternative names

    • Angiopoietin like 4 antibody
    • Angiopoietin related protein 4 antibody
    • Angiopoietin-like protein 4 antibody
    • Angiopoietin-related protein 4 antibody
    • ANGL4_HUMAN antibody
    • ANGPT L2 antibody
    • ANGPT L4 antibody
    • ANGPTL2 antibody
    • Angptl4 antibody
    • ARP4 antibody
    • Fasting induced adipose factor antibody
    • FIAF antibody
    • HARP antibody
    • Hepatic angiopoietin related protein antibody
    • Hepatic fibrinogen/angiopoietin related protein antibody
    • Hepatic fibrinogen/angiopoietin-related protein antibody
    • HFARP antibody
    • NL2 antibody
    • Peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin related protein antibody
    • PGAR antibody
    • pp1158 antibody
    • PPARG angiopoietin related protein antibody
    • PSEC0166 antibody
    • TGQTL antibody
    • UNQ171 antibody
    • Weakly similar to angiopoietin 1 [H.sapiens] antibody
    see all

Images

  • Lanes 1-3 : Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420) at 1/2000 dilution
    Lanes 4-5 : Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420) at 1/1000 dilution

    Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
    Lane 2 : SW480 (human colorectal adenocarcinoma cell line) whole cell lysate
    Lane 3 : THP-1 (human monocytic leukemia cell line) whole cell lysate
    Lane 4 : Human kidney lysate
    Lane 5 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lanes 4-5 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 45 kDa
    Observed band size: 35,50 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1-4: 3 minutes; Lane 5: 5 seconds.

    Angiopoietin-like 4/ANGPTL4 can be cleaved at 161-164 site and this immunogen recognize full length 50KD and C-Terminal fragment 35KD. (PMID:19270337; PMID:10862772).

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SW480 (human colorectal adenocarcinoma cell line) cells labeling Angiopoietin-like 4/ANGPTL4 with ab206420 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on SW480 cell line. The nuclear counterstain is DAPI (blue).

    Alpha-Tubulin is detected with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed HT-29 (human colorectal adenocarcinoma cell line) cells labeling Angiopoietin-like 4/ANGPTL4 with ab206420 at 1/700 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

  • Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420) at 1/1000 dilution + HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 45 kDa
    Observed band size: 35,50 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-29 (human colorectal adenocarcinoma cell line) cells labeling Angiopoietin-like 4/ANGPTL4 with ab206420 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HT-29 cell line. The nuclear counterstain is DAPI (blue).

    Alpha-Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

References

ab206420 has not yet been referenced specifically in any publications.

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