Anti-Angiostatin antibody (ab2904)
- Datasheet
- References (9)
- Protocols
Overview
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Product nameAnti-Angiostatin antibody
See all Angiostatin primary antibodies -
DescriptionRabbit polyclonal to Angiostatin
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Host speciesRabbit
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SpecificityDetects recombinant human angiostatin.
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Tested applicationsSuitable for: ICC, IHC-Fr, WB, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide corresponding to Mouse Angiostatin aa 98-116.
Sequence:VYLSECKTGIGNGYRGTMS
(Peptide available asab4925)
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab2904 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | 1/10 - 1/100. | |
| IHC-Fr | Use at an assay dependent concentration. PubMed: 17496220MOPS fixed frozen tissue. | |
| WB | Use a concentration of 2 µg/ml. Detects a band of approximately 38, 50 kDa (predicted molecular weight: 38 , 50 kDa).Can be blocked with Plasminogen peptide (ab4925). | |
| ICC/IF | Use at an assay dependent concentration. |
Target
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RelevanceAngiostatin is an internal proteolytic fragment of plasminogen, containing at least three Kringle domains. The Kringle domains have specific anti-migratory and anti-proliferative activities. Angiostatin, an endogenous angiogenesis inhibitor, inhibits endothelial cell proliferation in vitro and angiogenesis in vivo. It is a potent and specific natural inhibitor of angiogenisis and metastatic tumor growth.
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Cellular localizationSecreted
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Database links
- Entrez Gene: 5340 Human
- Entrez Gene: 18815 Mouse
- Entrez Gene: 85253 Rat
- Omim: 173350 Human
- SwissProt: P00747 Human
- SwissProt: P20918 Mouse
- SwissProt: Q01177 Rat
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Angiostatin antibody (ab2904)Immunocytochemistry/Immunofluorescence analysis of Angiostatin (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2904 in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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Immunocytochemistry/ Immunofluorescence - Anti-Angiostatin antibody (ab2904)Immunocytochemistry/Immunofluorescence analysis of Angiostatin (green) showing staining in the cytoplasm of K562 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab2904 in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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Western blot - Anti-Angiostatin antibody (ab2904)All lanes : Anti-Angiostatin antibody (ab2904) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Human Plasma Total Protein Lysate
Lane 3 : Human testis tissue lysate - total protein (ab30257)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 , 50 kDa
Observed band size: 38 + 50 kDa why is the actual band size different from the predicted?
Additional bands at: 27 kDa, 48 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
Protocols
Datasheets and documents
References
This product has been referenced in:
- Montagner A et al. Src is activated by the nuclear receptor peroxisome proliferator-activated receptor ß/d in ultraviolet radiation-induced skin cancer. EMBO Mol Med 6:80-98 (2014). IF ; Mouse . Read more (PubMed: 24203162) »
- Wu T et al. Urinary angiostatin--a novel putative marker of renal pathology chronicity in lupus nephritis. Mol Cell Proteomics 12:1170-9 (2013). Human . Read more (PubMed: 23345539) »
