Overview

  • Product name

    Angiotensin A ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Buffer 20 1297.9pg/ml 7.5%
    Buffer 20 655.4pg/ml 4.3%
    Buffer 20 55.2pg/ml 10.3%
    Inter-assay
    Sample n Mean SD CV%
    Buffer 1248.2pg/ml 10.9%
    Buffer 612.2pg/ml 5.2%
    Buffer 55.6pg/ml 17.7%
  • Sample type

    Serum, Plasma
  • Assay type

    Competitive
  • Sensitivity

    8.3 pg/ml
  • Range

    9.8 pg/ml - 10000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 94 50pg/ml - 5000pg/ml
    Plasma 109 50pg/ml - 5000pg/ml

  • Assay time

    3h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Angiotensin A in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Angiotensin A in plasma and serum.

    A goat anti-rabbit IgG polyclonal antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with a solution of Angiotensin A conjugated to biotin, followed by a solution of polyclonal antibody to Angiotensin A. The plate is washed to remove unbound reagents. A solution of streptavidin-HRP conjugate is then added. After further incubation the excess reagents are washed away and TMB substrate is added, which is catalyzed by HRP to generate a yellow color. A stop solution changes this color from yellow to blue, and the intensity of this blue coloration is inversely proportional to the amount of Angiotensin A captured in the plate.

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab136935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab136935.

  • Dose‐response curves from human plasma and serum diluted into assay buffer were compared to the Angiotensin A standard curve. The parallel response indicates the standard effectively mimics the native protein.

Protocols

References

ab136935 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab136935.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up