Anti-Angiotensin Converting Enzyme 1 antibody (ab28311)

Thank you for your telephone call earlier today. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

As discussed on the phone, I can confirm that ab28311 is tested and covered by our guarantee in WB and human samples. In and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

I would like to investigate this particular case further for you, and also obtain more information for our quality monitoringrecords. In order to proceed with this, I have enclosed atechnical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

With regards to the positive controls, Angiotensin Converting Enzyme 1 is known to be highly expressed in lung, kidney and heart tissue. We do havesomelysated available in our catalog:

Lung:
ab29118 Lung: right lower lobe (Human) Whole Cell Lysate - adult normal tissue
https://www.abcam.com/Human-lung-right-lower-lobe-tissue-lysate-total-protein-ab29118.html (or use the following: https://www.abcam.com/Human-lung-right-lower-lobe-tissue-lysate-total-protein-ab29118.html).

Kidney:
ab30203 Kidney (Human) Tissue Lysate - adult normal tissue
https://www.abcam.com/Human-kidney-tissue-lysate-total-protein-ab30203.html (or use the following: https://www.abcam.com/Human-kidney-tissue-lysate-total-protein-ab30203.html).

Heart:
ab29431Heart (Human) Tissue Lysate - adult normal tissue
https://www.abcam.com/Human-heart-tissue-lysate-total-protein-ab29431.html (or use the following: https://www.abcam.com/Human-heart-tissue-lysate-total-protein-ab29431.html).

Thank you for your time and cooperation. We look forward to receiving the completed questionaire.



Order Details
Antibody code:

Problem
Choose: Non-specific band Multiple bands No signal or weak signal High background

Lot number

Purchase order number
or preferably Abcam order number:



General Information
Antibody storage conditions (temperature/reconstitution etc)


Description of the problem (high background, wrong band size, more bands, no band etc.)


Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.)


Sample preparation (Buffer/Protease inhibitors/Heating sample etc.)


Amount of protein loaded


Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.)


Transfer and blocking conditions (Buffer/time period, Blocking agent etc.)


Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Detection method (ECL, ECLPlus etc.)


Positive and negative controls used (please specify)



Optimization attempts (problem solving)
How many times have you tried the Western?



Have you run a "No Primary" control?
Yes No

Do you obtain the same results every time?
Yes No
e.g. are the background bands always in the same place?


What steps have you altered?


Additional Notes:


Image:
We would appreciate if you are able to provide an image (including molecular weight markers) which would help us to asses the results.

Thank you for your telephone call. As discussed, I am sorry that as we carry over 70,000 products, it is not feasible for us to keep small sample sizes of our products to send to customers. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase. In this case, as GMA embedding is quite similar to IHC-P, I am pleased to say we will offer an exception in this case and cover these two antibodies (ab28311 and ab76526) with our Abpromise guarantee for GMA embedded sections.   Also, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates. To find out more about our Abreview system, please see the following link: https://www.abcam.com/abreviews Although we do not provide institutional discount in the UK at present, we are able to provide discounts if you purchase 5 items or more on one order. In order to find out more, I can recommend to send a list of the items you wish to order to our customer service department. They can then send a quote and proforma invoice.  You can contact them at orders@abcam.com. I hope this information is helpful. Please do not hesitate to contact us again with any other questions.