Anti-Angiotensin Converting Enzyme 1 antibody (ab28311)

Overview

  • Product name
    Anti-Angiotensin Converting Enzyme 1 antibody
    See all Angiotensin Converting Enzyme 1 primary antibodies
  • Description
    Rabbit polyclonal to Angiotensin Converting Enzyme 1
  • Host species
    Rabbit
  • Specificity
    ab28311 recognizes metallopeptidase ACE1.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    The antibody is made to a synthetic peptide based on the first peptidase unit of human ACE1 (Human).

    Read Abcam's proprietary immunogen policy (Peptide available as ab41278.)

  • Positive control
    • ACE1 is found in the lung, kidney, heart, pancreas, prostate, testis, small intestine and colon, and is found in cell lysates and as shed or processed forms in cell culture media and in biological fluids.

Properties

Applications

Our Abpromise guarantee covers the use of ab28311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 150 kDa. Dilution optimised using Chromogenic detection
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function
    Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.
  • Tissue specificity
    Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis.
  • Involvement in disease
    Ischemic stroke (ISCHSTR) [MIM:601367]: A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
    Renal tubular dysgenesis (RTD) [MIM:267430]: Autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Note=The disease is caused by mutations affecting the gene represented in this entry.
    Microvascular complications of diabetes 3 (MVCD3) [MIM:612624]: Pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
    Intracerebral hemorrhage (ICH) [MIM:614519]: A pathological condition characterized by bleeding into one or both cerebral hemispheres including the basal ganglia and the cerebral cortex. It is often associated with hypertension and craniocerebral trauma. Intracerebral bleeding is a common cause of stroke. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
  • Sequence similarities
    Belongs to the peptidase M2 family.
  • Post-translational
    modifications
    Phosphorylated by CK2 on Ser-1299; which allows membrane retention.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACE 1 antibody
    • ACE antibody
    • ACE T antibody
    • ACE_HUMAN antibody
    • ACE1 antibody
    • Angiotensin converting enzyme somatic isoform antibody
    • Angiotensin converting enzyme testis specific isoform antibody
    • Angiotensin I converting enzyme 1 antibody
    • Angiotensin I converting enzyme antibody
    • Angiotensin I converting enzyme peptidyl dipeptidase A 1 antibody
    • angiotensin I converting enzyme peptidyl-dipeptidase A 1 transcript antibody
    • Angiotensin-converting enzyme antibody
    • Carboxycathepsin antibody
    • CD 143 antibody
    • CD143 antibody
    • CD143 antigen antibody
    • DCP 1 antibody
    • DCP antibody
    • DCP1 antibody
    • Dipeptidyl carboxypeptidase 1 antibody
    • Dipeptidyl carboxypeptidase I antibody
    • Kininase II antibody
    • MGC26566 antibody
    • MVCD3 antibody
    • Peptidase P antibody
    • Peptidyl dipeptidase A antibody
    • soluble form antibody
    • Testicular ECA antibody
    see all

Images

  • Lane 1 : Molecular weight markers
    Lanes 2-4 : Anti-Angiotensin Converting Enzyme 1 antibody (ab28311) at 1/1000 dilution

    Lane 2 : ACE1 at 0.05 µg
    Lane 3 : ACE1 at 0.01 µg
    Lane 4 : ACE1 at 0.001 µg

    Observed band size: 150 kDa
    why is the actual band size different from the predicted?

  • ab28311 staining Angiotensin Converting Enzyme 1 antibody - Aminoterminal end catalytic domain in human mesenteric artery tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in paraformaldehyde and a heat mediated antigen retrieval step was performed using TRIS pH 9.0, 123°C for 1 minute. Samples were then permeabilized using 0.1% Triton, blocked with 3% BSA for 30 minutes at 25°C and then incubated with ab28311 at a 1/500 dilution for 16 hours at 4°C. The secondary used was an undiluted goat anti-rabbit polyclonal-HRP.

    See Abreview

References

This product has been referenced in:
  • Matsuura-Hachiya Y  et al. Angiotensin-converting enzyme inhibitor (enalapril maleate) accelerates recovery of mouse skin from UVB-induced wrinkles. Biochem Biophys Res Commun 442:38-43 (2013). IHC-P ; Mouse . Read more (PubMed: 24216112) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Question
Answer

Thank you for your telephone call earlier today. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

As discussed on the phone, I can confirm that ab28311 is tested and covered by our guarantee in WB and human samples. In and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

I would like to investigate this particular case further for you, and also obtain more information for our quality monitoringrecords. In order to proceed with this, I have enclosed atechnical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

With regards to the positive controls, Angiotensin Converting Enzyme 1 is known to be highly expressed in lung, kidney and heart tissue. We do havesomelysated available in our catalog:

Lung:
ab29118 Lung: right lower lobe (Human) Whole Cell Lysate - adult normal tissue
https://www.abcam.com/index.html?datasheet=29118 (or use the following: https://www.abcam.com/index.html?datasheet=29118).

Kidney:
ab30203 Kidney (Human) Tissue Lysate - adult normal tissue
https://www.abcam.com/index.html?datasheet=30203 (or use the following: https://www.abcam.com/index.html?datasheet=30203).

Heart:
ab29431Heart (Human) Tissue Lysate - adult normal tissue
https://www.abcam.com/index.html?datasheet=29431 (or use the following: https://www.abcam.com/index.html?datasheet=29431).

Thank you for your time and cooperation. We look forward to receiving the completed questionaire.



Order Details
Antibody code:

Problem
Choose: Non-specific band Multiple bands No signal or weak signal High background

Lot number

Purchase order number
or preferably Abcam order number:



General Information
Antibody storage conditions (temperature/reconstitution etc)


Description of the problem (high background, wrong band size, more bands, no band etc.)


Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.)


Sample preparation (Buffer/Protease inhibitors/Heating sample etc.)


Amount of protein loaded


Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.)


Transfer and blocking conditions (Buffer/time period, Blocking agent etc.)


Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Detection method (ECL, ECLPlus etc.)


Positive and negative controls used (please specify)



Optimization attempts (problem solving)
How many times have you tried the Western?



Have you run a "No Primary" control?
Yes No

Do you obtain the same results every time?
Yes No
e.g. are the background bands always in the same place?


What steps have you altered?


Additional Notes:


Image:
We would appreciate if you are able to provide an image (including molecular weight markers) which would help us to asses the results.

Read More

Answer

Thank you for your telephone call. As discussed, I am sorry that as we carry over 70,000 products, it is not feasible for us to keep small sample sizes of our products to send to customers. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase. In this case, as GMA embedding is quite similar to IHC-P, I am pleased to say we will offer an exception in this case and cover these two antibodies (ab28311 and ab76526) with our Abpromise guarantee for GMA embedded sections.   Also, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates. To find out more about our Abreview system, please see the following link: https://www.abcam.com/abreviews Although we do not provide institutional discount in the UK at present, we are able to provide discounts if you purchase 5 items or more on one order. In order to find out more, I can recommend to send a list of the items you wish to order to our customer service department. They can then send a quote and proforma invoice.  You can contact them at orders@abcam.com. I hope this information is helpful. Please do not hesitate to contact us again with any other questions.  

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Mouse cells transfected with human gene)
Loading amount
20 µg
Specification
Mouse cells transfected with human gene
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Gillian Hunter

Verified customer

Submitted Jul 28 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Mesenteric artery)
Specification
Mesenteric artery
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris pH9, 123ºC, 1 minute
Permeabilization
Yes - PBS-0.1%Triton 10 minutes
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C

Dr. Javier Triñanes

Verified customer

Submitted Jul 02 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up