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Cardiovascular Blood Serum Proteins
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Anti-Angiotensin Converting Enzyme 1 antibody (ab28311)

  • Datasheet
  • SDS
Reviews (2)Q&A (2)References (3)

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Western blot - Anti-Angiotensin Converting Enzyme 1 antibody (ab28311)

    Key features and details

    • Rabbit polyclonal to Angiotensin Converting Enzyme 1
    • Suitable for: WB
    • Reacts with: Recombinant fragment
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human Angiotensin Converting Enzyme 1 protein (ab158266)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-Angiotensin Converting Enzyme 1 antibody
      See all Angiotensin Converting Enzyme 1 primary antibodies
    • Description

      Rabbit polyclonal to Angiotensin Converting Enzyme 1
    • Host species

      Rabbit
    • Specificity

      ab28311 recognizes metallopeptidase ACE1.
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Recombinant fragment
    • Immunogen

      The antibody is made to a synthetic peptide based on the first peptidase unit of human ACE1 (Human).

      Read Abcam's proprietary immunogen policy (Peptide available as ab41278.)

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      Preservative: 0.05% Sodium azide
      Constituents: PBS, 50% Glycerol
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      The antibody has been peptide-affinity purified and concentrated.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cardiovascular
      • Blood
      • Serum Proteins
      • Stem Cells
      • Hematopoietic Progenitors
      • Surface Molecules
      • Signal Transduction
      • Metabolism
      • Vitamins / Minerals
      • Cell Biology
      • Proteolysis / Ubiquitin
      • Proteolytic enzymes
      • Metalloprotease
      • ACEs
      • Cardiovascular
      • Vasculature
      • Vasoconstriction
      • Other
      • Cancer
      • Cancer Metabolism
      • Response to hypoxia
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Cardiovascular ELISA kits
      • Metabolism
      • Pathways and Processes
      • Cofactors, Vitamins / minerals
      • Vitamins / minerals
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Hypoxia
      • Metabolism
      • Types of disease
      • Cancer

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Angiotensin Converting Enzyme 1 protein (ab158266)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab28311 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (1)
    1/1000. when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 150 kDa. Dilution optimised using Chromogenic detection
    Notes
    WB
    1/1000. when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 150 kDa. Dilution optimised using Chromogenic detection

    Target

    • Function

      Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.
    • Tissue specificity

      Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis.
    • Involvement in disease

      Ischemic stroke (ISCHSTR) [MIM:601367]: A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
      Renal tubular dysgenesis (RTD) [MIM:267430]: Autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Note=The disease is caused by mutations affecting the gene represented in this entry.
      Microvascular complications of diabetes 3 (MVCD3) [MIM:612624]: Pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
      Intracerebral hemorrhage (ICH) [MIM:614519]: A pathological condition characterized by bleeding into one or both cerebral hemispheres including the basal ganglia and the cerebral cortex. It is often associated with hypertension and craniocerebral trauma. Intracerebral bleeding is a common cause of stroke. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
    • Sequence similarities

      Belongs to the peptidase M2 family.
    • Post-translational
      modifications

      Phosphorylated by CK2 on Ser-1299; which allows membrane retention.
    • Cellular localization

      Secreted and Cell membrane.
    • Target information above from: UniProt accession P12821 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • ACE 1 antibody
      • ACE antibody
      • ACE T antibody
      • ACE_HUMAN antibody
      • ACE1 antibody
      • Angiotensin converting enzyme somatic isoform antibody
      • Angiotensin converting enzyme testis specific isoform antibody
      • Angiotensin I converting enzyme 1 antibody
      • Angiotensin I converting enzyme antibody
      • Angiotensin I converting enzyme peptidyl dipeptidase A 1 antibody
      • angiotensin I converting enzyme peptidyl-dipeptidase A 1 transcript antibody
      • Angiotensin-converting enzyme antibody
      • Carboxycathepsin antibody
      • CD 143 antibody
      • CD143 antibody
      • CD143 antigen antibody
      • DCP 1 antibody
      • DCP antibody
      • DCP1 antibody
      • Dipeptidyl carboxypeptidase 1 antibody
      • Dipeptidyl carboxypeptidase I antibody
      • Kininase II antibody
      • MGC26566 antibody
      • MVCD3 antibody
      • Peptidase P antibody
      • Peptidyl dipeptidase A antibody
      • soluble form antibody
      • Testicular ECA antibody
      see all

    Images

    • Western blot - Anti-Angiotensin Converting Enzyme 1 antibody (ab28311)
      Western blot - Anti-Angiotensin Converting Enzyme 1 antibody (ab28311)
      Lane 1 : Molecular weight markers
      Lanes 2-4 : Anti-Angiotensin Converting Enzyme 1 antibody (ab28311) at 1/1000 dilution

      Lane 2 : ACE1 at 0.05 µg
      Lane 3 : ACE1 at 0.01 µg
      Lane 4 : ACE1 at 0.001 µg

      Observed band size: 150 kDa
      why is the actual band size different from the predicted?

    Protocols

    • Recommended WB protocol

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (3)

    Publishing research using ab28311? Please let us know so that we can cite the reference in this datasheet.

    ab28311 has been referenced in 3 publications.

    • Kidoguchi S  et al. Azilsartan causes natriuresis due to its sympatholytic action in kidney disease. Hypertens Res 42:1507-1517 (2019). PubMed: 31138899
    • Zhang Y  et al. Comparative Expression of Renin-Angiotensin Pathway Proteins in Visceral Versus Subcutaneous Fat. Front Physiol 9:1370 (2018). PubMed: 30364113
    • Matsuura-Hachiya Y  et al. Angiotensin-converting enzyme inhibitor (enalapril maleate) accelerates recovery of mouse skin from UVB-induced wrinkles. Biochem Biophys Res Commun 442:38-43 (2013). IHC-P ; Mouse . PubMed: 24216112

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Question

    ab28311 is not working well in WB.
    Could you suggest some positive controls?

    Read More

    Abcam community

    Verified customer

    Asked on Jan 16 2012

    Answer

    Thank you for your telephone call earlier today. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    As discussed on the phone, I can confirm that ab28311 is tested and covered by our guarantee in WB and human samples. In and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

    I would like to investigate this particular case further for you, and also obtain more information for our quality monitoringrecords. In order to proceed with this, I have enclosed atechnical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

    With regards to the positive controls, Angiotensin Converting Enzyme 1 is known to be highly expressed in lung, kidney and heart tissue. We do havesomelysated available in our catalog:

    Lung:
    ab29118 Lung: right lower lobe (Human) Whole Cell Lysate - adult normal tissue
    https://www.abcam.com/Human-lung-right-lower-lobe-tissue-lysate-total-protein-ab29118.html (or use the following: https://www.abcam.com/Human-lung-right-lower-lobe-tissue-lysate-total-protein-ab29118.html).

    Kidney:
    ab30203 Kidney (Human) Tissue Lysate - adult normal tissue
    https://www.abcam.com/Human-kidney-tissue-lysate-total-protein-ab30203.html (or use the following: https://www.abcam.com/Human-kidney-tissue-lysate-total-protein-ab30203.html).

    Heart:
    ab29431Heart (Human) Tissue Lysate - adult normal tissue
    https://www.abcam.com/Human-heart-tissue-lysate-total-protein-ab29431.html (or use the following: https://www.abcam.com/Human-heart-tissue-lysate-total-protein-ab29431.html).

    Thank you for your time and cooperation. We look forward to receiving the completed questionaire.



    Order Details
    Antibody code:

    Problem
    Choose: Non-specific band Multiple bands No signal or weak signal High background

    Lot number

    Purchase order number
    or preferably Abcam order number:



    General Information
    Antibody storage conditions (temperature/reconstitution etc)


    Description of the problem (high background, wrong band size, more bands, no band etc.)


    Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.)


    Sample preparation (Buffer/Protease inhibitors/Heating sample etc.)


    Amount of protein loaded


    Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.)


    Transfer and blocking conditions (Buffer/time period, Blocking agent etc.)


    Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


    Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


    Detection method (ECL, ECLPlus etc.)


    Positive and negative controls used (please specify)



    Optimization attempts (problem solving)
    How many times have you tried the Western?



    Have you run a "No Primary" control?
    Yes No

    Do you obtain the same results every time?
    Yes No
    e.g. are the background bands always in the same place?


    What steps have you altered?


    Additional Notes:


    Image:
    We would appreciate if you are able to provide an image (including molecular weight markers) which would help us to asses the results.

    Read More

    Abcam Scientific Support

    Answered on Jan 16 2012

    Question

    Can we have a free sample of ab28311 and ab76526? We want to try in GMA embedded human tissue. Does **** University get a discount?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 22 2011

    Answer

    Thank you for your telephone call. As discussed, I am sorry that as we carry over 70,000 products, it is not feasible for us to keep small sample sizes of our products to send to customers. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase. In this case, as GMA embedding is quite similar to IHC-P, I am pleased to say we will offer an exception in this case and cover these two antibodies (ab28311 and ab76526) with our Abpromise guarantee for GMA embedded sections.   Also, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates. To find out more about our Abreview system, please see the following link: https://www.abcam.com/abreviews Although we do not provide institutional discount in the UK at present, we are able to provide discounts if you purchase 5 items or more on one order. In order to find out more, I can recommend to send a list of the items you wish to order to our customer service department. They can then send a quote and proforma invoice.  You can contact them at orders@abcam.com. I hope this information is helpful. Please do not hesitate to contact us again with any other questions.  

    Read More

    Abcam Scientific Support

    Answered on Nov 22 2011

    Western blot abreview for Anti-Angiotensin Converting Enzyme 1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Mouse cells transfected with human gene)
    Loading amount
    20 µg
    Specification
    Mouse cells transfected with human gene
    Gel Running Conditions
    Reduced Denaturing (8%)
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. Gillian Hunter

    Verified customer

    Submitted Jul 28 2011

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Angiotensin Converting Enzyme 1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Mesenteric artery)
    Specification
    Mesenteric artery
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris pH9, 123ºC, 1 minute
    Permeabilization
    Yes - PBS-0.1%Triton 10 minutes
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Dr. Javier Triñanes

    Verified customer

    Submitted Jul 02 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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