Anti-Angiotensin Converting Enzyme 1 antibody (ab28311)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-Angiotensin Converting Enzyme 1 antibody
See all Angiotensin Converting Enzyme 1 primary antibodies -
DescriptionRabbit polyclonal to Angiotensin Converting Enzyme 1
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Host speciesRabbit
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Specificityab28311 recognizes metallopeptidase ACE1.
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Tested applicationsSuitable for: WB, IHC-Pmore details
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Species reactivityReacts with: Mouse, Human
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Immunogen
The antibody is made to a synthetic peptide based on the first peptidase unit of human ACE1 (Human).
(Peptide available as ab41278.)
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Positive control
- ACE1 is found in the lung, kidney, heart, pancreas, prostate, testis, small intestine and colon, and is found in cell lysates and as shed or processed forms in cell culture media and in biological fluids.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 50% Sucrose, PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesThe antibody has been peptide-affinity purified and concentrated.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab28311 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 150 kDa. Dilution optimised using Chromogenic detection | |
| IHC-P | 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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FunctionConverts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.
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Tissue specificityUbiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis.
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Involvement in diseaseIschemic stroke (ISCHSTR) [MIM:601367]: A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
Renal tubular dysgenesis (RTD) [MIM:267430]: Autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Note=The disease is caused by mutations affecting the gene represented in this entry.
Microvascular complications of diabetes 3 (MVCD3) [MIM:612624]: Pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
Intracerebral hemorrhage (ICH) [MIM:614519]: A pathological condition characterized by bleeding into one or both cerebral hemispheres including the basal ganglia and the cerebral cortex. It is often associated with hypertension and craniocerebral trauma. Intracerebral bleeding is a common cause of stroke. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry. -
Sequence similaritiesBelongs to the peptidase M2 family.
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Post-translational
modificationsPhosphorylated by CK2 on Ser-1299; which allows membrane retention. -
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 1636 Human
- Entrez Gene: 11421 Mouse
- Omim: 106180 Human
- SwissProt: P12821 Human
- SwissProt: P09470 Mouse
- Unigene: 298469 Human
- Unigene: 754 Mouse
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Alternative names
- ACE 1 antibody
- ACE antibody
- ACE T antibody
see all
Images
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Western blot - Anti-Angiotensin Converting Enzyme 1 antibody (ab28311)Lane 1 : Molecular weight markers
Lanes 2-4 : Anti-Angiotensin Converting Enzyme 1 antibody (ab28311) at 1/1000 dilution
Lane 2 : ACE1 at 0.05 µg
Lane 3 : ACE1 at 0.01 µg
Lane 4 : ACE1 at 0.001 µg
Observed band size: 150 kDa (why is the actual band size different from the predicted?) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody (ab28311)Image courtesy of Dr Javier Triñanes by Abreview.ab28311 staining Angiotensin Converting Enzyme 1 antibody - Aminoterminal end catalytic domain in human mesenteric artery tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed in paraformaldehyde and a heat mediated antigen retrieval step was performed using TRIS pH 9.0, 123°C for 1 minute. Samples were then permeabilized using 0.1% Triton, blocked with 3% BSA for 30 minutes at 25°C and then incubated with ab28311 at a 1/500 dilution for 16 hours at 4°C. The secondary used was an undiluted goat anti-rabbit polyclonal-HRP.
Protocols
References
This product has been referenced in:
- Matsuura-Hachiya Y et al. Angiotensin-converting enzyme inhibitor (enalapril maleate) accelerates recovery of mouse skin from UVB-induced wrinkles. Biochem Biophys Res Commun 442:38-43 (2013). IHC-P ; Mouse . Read more (PubMed: 24216112) »
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