Anti-Angiotensin Converting Enzyme 1 antibody (ab28311)
Key features and details
- Rabbit polyclonal to Angiotensin Converting Enzyme 1
- Suitable for: WB, IHC-P
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Angiotensin Converting Enzyme 1 antibody
See all Angiotensin Converting Enzyme 1 primary antibodies -
Description
Rabbit polyclonal to Angiotensin Converting Enzyme 1 -
Host species
Rabbit -
Specificity
ab28311 recognizes metallopeptidase ACE1. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
The antibody is made to a synthetic peptide based on the first peptidase unit of human ACE1 (Human).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody has been peptide-affinity purified and concentrated. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28311 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000. when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 150 kDa. Dilution optimised using Chromogenic detection
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IHC-P | (1) |
Use at an assay dependent concentration.
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Notes |
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WB
1/1000. when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 150 kDa. Dilution optimised using Chromogenic detection |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. -
Tissue specificity
Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis. -
Involvement in disease
Ischemic stroke (ISCHSTR) [MIM:601367]: A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
Renal tubular dysgenesis (RTD) [MIM:267430]: Autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Note=The disease is caused by mutations affecting the gene represented in this entry.
Microvascular complications of diabetes 3 (MVCD3) [MIM:612624]: Pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
Intracerebral hemorrhage (ICH) [MIM:614519]: A pathological condition characterized by bleeding into one or both cerebral hemispheres including the basal ganglia and the cerebral cortex. It is often associated with hypertension and craniocerebral trauma. Intracerebral bleeding is a common cause of stroke. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry. -
Sequence similarities
Belongs to the peptidase M2 family. -
Post-translational
modificationsPhosphorylated by CK2 on Ser-1299; which allows membrane retention. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1636 Human
- Omim: 106180 Human
- SwissProt: P12821 Human
- Unigene: 298469 Human
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Alternative names
- ACE 1 antibody
- ACE antibody
- ACE T antibody
see all
Images
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Lane 1 : Molecular weight markers
Lanes 2-4 : Anti-Angiotensin Converting Enzyme 1 antibody (ab28311) at 1/1000 dilution
Lane 2 : rh-ACE1 at 0.05 µg
Lane 3 : rh-ACE1 at 0.01 µg
Lane 4 : rh-ACE1 at 0.001 µg
Observed band size: 150 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab28311 has been referenced in 7 publications.
- Shang F et al. Endothelial MicroRNA-483-3p Is Hypertension-Protective. Oxid Med Cell Longev 2022:3698219 (2022). PubMed: 35222797
- Ma R et al. Protective effects of irbesartan and benazepril against vaginal vascular remodeling and fibrosis in female spontaneously hypertensive rats. J Int Med Res 48:300060520943453 (2020). PubMed: 32790534
- Kidoguchi S et al. Azilsartan causes natriuresis due to its sympatholytic action in kidney disease. Hypertens Res 42:1507-1517 (2019). PubMed: 31138899
- Joshi S et al. Hypoxic regulation of angiotensin-converting enzyme 2 and Mas receptor in human CD34+ cells. J Cell Physiol N/A:N/A (2019). PubMed: 30989646
- Zhang Y et al. Comparative Expression of Renin-Angiotensin Pathway Proteins in Visceral Versus Subcutaneous Fat. Front Physiol 9:1370 (2018). PubMed: 30364113
- Matsuura-Hachiya Y et al. Angiotensin-converting enzyme inhibitor (enalapril maleate) accelerates recovery of mouse skin from UVB-induced wrinkles. Biochem Biophys Res Commun 442:38-43 (2013). IHC-P ; Mouse . PubMed: 24216112
- Urushihara M et al. Glomerular angiotensin-converting enzyme 2 in pediatric IgA nephropathy. Am J Nephrol 38:355-67 (2013). PubMed: 24158104