Recombinant
RabMAb

Recombinant Anti-Angiotensin Converting Enzyme 1 antibody [EPR22250-204] (ab222739)

Overview

  • Product name

    Anti-Angiotensin Converting Enzyme 1 antibody [EPR22250-204]
    See all Angiotensin Converting Enzyme 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR22250-204] to Angiotensin Converting Enzyme 1
  • Host species

    Rabbit
  • Specificity

    WB is only recommended for mouse.
  • Tested applications

    Suitable for: WB, IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Mouse Angiotensin Converting Enzyme 1 aa 600-1250. The exact sequence is proprietary.
    Database link: P09470

  • Positive control

    • WB: Mouse liver, brain, heart, kidney and lung lysates; bEnd.3 whole cell lysate. IHC-P: Mouse and rat lung tissues. IHC-Fr: Mouse kidney tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab222739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 180 kDa (predicted molecular weight: 151 kDa).
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr 1/100.

Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Target

  • Function

    Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.
  • Tissue specificity

    Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis.
  • Involvement in disease

    Ischemic stroke (ISCHSTR) [MIM:601367]: A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
    Renal tubular dysgenesis (RTD) [MIM:267430]: Autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Note=The disease is caused by mutations affecting the gene represented in this entry.
    Microvascular complications of diabetes 3 (MVCD3) [MIM:612624]: Pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
    Intracerebral hemorrhage (ICH) [MIM:614519]: A pathological condition characterized by bleeding into one or both cerebral hemispheres including the basal ganglia and the cerebral cortex. It is often associated with hypertension and craniocerebral trauma. Intracerebral bleeding is a common cause of stroke. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
  • Sequence similarities

    Belongs to the peptidase M2 family.
  • Post-translational
    modifications

    Phosphorylated by CK2 on Ser-1299; which allows membrane retention.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACE 1 antibody
    • ACE antibody
    • ACE T antibody
    • ACE_HUMAN antibody
    • ACE1 antibody
    • Angiotensin converting enzyme somatic isoform antibody
    • Angiotensin converting enzyme testis specific isoform antibody
    • Angiotensin I converting enzyme 1 antibody
    • Angiotensin I converting enzyme antibody
    • Angiotensin I converting enzyme peptidyl dipeptidase A 1 antibody
    • angiotensin I converting enzyme peptidyl-dipeptidase A 1 transcript antibody
    • Angiotensin-converting enzyme antibody
    • Carboxycathepsin antibody
    • CD 143 antibody
    • CD143 antibody
    • CD143 antigen antibody
    • DCP 1 antibody
    • DCP antibody
    • DCP1 antibody
    • Dipeptidyl carboxypeptidase 1 antibody
    • Dipeptidyl carboxypeptidase I antibody
    • Kininase II antibody
    • MGC26566 antibody
    • MVCD3 antibody
    • Peptidase P antibody
    • Peptidyl dipeptidase A antibody
    • soluble form antibody
    • Testicular ECA antibody
    see all

Images

  • All lanes : Anti-Angiotensin Converting Enzyme 1 antibody [EPR22250-204] (ab222739) at 1/1000 dilution

    Lane 1 : Mouse liver lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Mouse heart lysate
    Lane 4 : Mouse kidney lysate
    Lane 5 : bEnd.3 (mouse brain endothelioma cell line) whole cell lysate
    Lane 6 : Mouse lung lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 151 kDa
    Observed band size: 180 kDa
    why is the actual band size different from the predicted?



    Exposure time : Lane 1: 81 seconds; Lane 2: 26 seconds; Lane 3: 8 seconds; Lane 4: 3 seconds; Lane 5: 48 seconds; Lane 6: 10 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Apart from the target band at 180-kDa, weak reactivity bands observed could be due to glycosylation (PMID: 24163131).

  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Angiotensin Converting Enzyme 1 with ab222739 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on endothelial cells of mouse lung (PMID: 19451697). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling Angiotensin Converting Enzyme 1 with ab222739  at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive staining on mouse renal tubules (PMID:25664248) is observed.

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Angiotensin Converting Enzyme 1 with ab222739 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on endothelial cells of rat lung (PMID: 19451697). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab222739 has not yet been referenced specifically in any publications.

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