Recombinant
RabMAb

Recombinant Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762)

Overview

  • Product name

    Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757]
    See all Angiotensin Converting Enzyme 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR2757] to Angiotensin Converting Enzyme 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    within Human Angiotensin Converting Enzyme 1 aa 1250 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P12821

  • Positive control

    • Fetal kidney, fetal heart and fetal lung lysates; human kidney and spleen tissues.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab75762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 195 kDa (predicted molecular weight: 150 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC or IP.
  • Target

    • Function

      Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.
    • Tissue specificity

      Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis.
    • Involvement in disease

      Ischemic stroke (ISCHSTR) [MIM:601367]: A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
      Renal tubular dysgenesis (RTD) [MIM:267430]: Autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Note=The disease is caused by mutations affecting the gene represented in this entry.
      Microvascular complications of diabetes 3 (MVCD3) [MIM:612624]: Pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
      Intracerebral hemorrhage (ICH) [MIM:614519]: A pathological condition characterized by bleeding into one or both cerebral hemispheres including the basal ganglia and the cerebral cortex. It is often associated with hypertension and craniocerebral trauma. Intracerebral bleeding is a common cause of stroke. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
    • Sequence similarities

      Belongs to the peptidase M2 family.
    • Post-translational
      modifications

      Phosphorylated by CK2 on Ser-1299; which allows membrane retention.
    • Cellular localization

      Secreted and Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • ACE 1 antibody
      • ACE antibody
      • ACE T antibody
      • ACE_HUMAN antibody
      • ACE1 antibody
      • Angiotensin converting enzyme somatic isoform antibody
      • Angiotensin converting enzyme testis specific isoform antibody
      • Angiotensin I converting enzyme 1 antibody
      • Angiotensin I converting enzyme antibody
      • Angiotensin I converting enzyme peptidyl dipeptidase A 1 antibody
      • angiotensin I converting enzyme peptidyl-dipeptidase A 1 transcript antibody
      • Angiotensin-converting enzyme antibody
      • Carboxycathepsin antibody
      • CD 143 antibody
      • CD143 antibody
      • CD143 antigen antibody
      • DCP 1 antibody
      • DCP antibody
      • DCP1 antibody
      • Dipeptidyl carboxypeptidase 1 antibody
      • Dipeptidyl carboxypeptidase I antibody
      • Kininase II antibody
      • MGC26566 antibody
      • MVCD3 antibody
      • Peptidase P antibody
      • Peptidyl dipeptidase A antibody
      • soluble form antibody
      • Testicular ECA antibody
      see all

    Images

    • All lanes : Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762) at 1/1000 dilution

      Lane 1 : fetal kidney lysate
      Lane 2 : fetal heart lysate
      Lane 3 : fetal lung lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 150 kDa
      Observed band size: 195 kDa
      why is the actual band size different from the predicted?

    • ab75762 at 1/100 dilution staining Angiotensin Converting Enzyme 1 in human kidney by Immunohistochemistry, Paraffin-embedded tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • ab75762 at 1/100 dilution staining Angiotensin Converting Enzyme 1 in human spleen by Immunohistochemistry, Paraffin-embedded tissue. Note positive staining of endothelial cells.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Sato T  et al. ELABELA-APJ axis protects from pressure overload heart failure and angiotensin II-induced cardiac damage. Cardiovasc Res 113:760-769 (2017). Read more (PubMed: 28371822) »
    • Yang J  et al. Pathological Ace2-to-Ace enzyme switch in the stressed heart is transcriptionally controlled by the endothelial Brg1-FoxM1 complex. Proc Natl Acad Sci U S A 113:E5628-35 (2016). Read more (PubMed: 27601681) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Answer

    Wie besprochen habe ich eine kostenlose Ersatzlieferung für Sie in Auftrag gegeben. Sie hat die Referenznummer 1155271 und sollte morgen bei Ihnen ankommen.

    Das Molekulargewicht kann man folgendermaßen errechnen: Öffnen Sie die SwissProt Link auf unserem Datenblatt für das Maus Protein (http://www.uniprot.org/uniprot/P09470), und rollen Sie die Seite einmal bis zum Abschnitt "Sequence annotation (Features)" herunter. Dort finden Sie die Angaben zum "Molecule processing" ,in welchen die Länge und Positionen der einzelnen Protein Ketten beschrieben werde. Wenn Sie die jeweilige Position öffnen, gelangen Sie zur FASTA Sequenz ( in dem Kasten oben links).

    Desweiteren oeffnen Sie den Molekular Gewicht/ pI Rechner unter dem folgenden Link http://web.expasy.org/compute_pi/

    In diesen fügen Sie die FASTA Sequenz ein, und lassen das Gewicht berechnen.

    Ich wünsche Ihnen viel Erfolg für Ihr Projekt. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

    Read More

    Question

    Danke für die schnelle Bearbeitung. Anbei das troubleshooting und drei ausgewählte blots meiner inkubierten Membran.
    Vielen lieben Dank im Vorraus,
    Marion Bisha
    1) Abcam product code ab11737 and ab75762

    2) Abcam order reference number or product batch number
    3) Description of the problem : wrong so signals
    4) Sample preparation:
    Type of sample (whole cell lysates, fraction, recombinant protein…): whole cell lysates, bEND 3 (brain endothelial cells)
    Lysis buffer : Rippa buffer 1ml, PIC 40µl, NaF 10 µl, NaP2O 10 µl, BGP 10 µl, NaV 5 µl
    Boiling for ≥5 min? no 5 min 95°C
    Protein loaded ug/lane 40 Mg / lane
    Positive control ACE rabbit
    Negative control
    5) Percentage of gel: 10%
    Type of membrane : PVDF
    Protein transfer verified
    Blocking agent and concentration; Odyssey Blocking buffer 50% / PBS 50%
    Blocking time : 1 hour
    Blocking temperature room temperature
    6) Primary antibody:
    Concentration or dilution : ab11737= 1:100 and 1:50 and 1:20 ab75762 = 1:1000
    Diluent buffer : Odyssey Blocking buffer 50% / PBS 50% plus 0,1 % Tween
    Incubation time: over night or 4 hours
    Incubation temperature: over night inn 4 °C and 4 hours at room temperature
    7) Secondary antibody: ab11737: anti mouse Odyssey antibody
    Ab75762 anti rabbit Odyssey antibody
    Species:
    Reacts against:
    Concentration or dilution 1:10000
    Diluent buffer : Odyssey Blocking buffer 50% / PBS 50% plus 0,1 % Tween
    Incubation time: 1 hour
    Incubation temperature: room temperature
    Fluorochrome or enzyme conjugate:
    8) Washing after primary and secondary antibodies:
    Buffer: between the antibodies incubation 4 x 5 min with Odyssey Blocking buffer 50% / PBS 50% plus 0,1 % Tween and after the incubation with the second antibody 2 x 5 min with Odyssey Blocking buffer 50% / PBS 50% plus 0,1 % Tween and 2 x 5 min with Odyssey Blocking buffer 50% / PBS 50%
    Number of washes
    9)Detection method
    10) How many times have you run this staining? 5 x
    Do you obtain the same results every time? Jes
    What steps have you altered to try and optimize the use of this antibody?
    Different concentration of the antibodies and different incubation times
    Document attachment: Attaching images of your blot is strongly recommended and can greatly speed up our investigation of your problem.

    Read More
    Answer

    Vielen Dank für Ihren Anruf und dafür, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen.



    Ich möchte folgende Bemerkungen zu Ihrem Protokoll machen:

    1. Ich denke, Sie detektieren das richtige Protein, da zwei so unterschiedliche Antikörper das gleiche Signal zeigen. Dies kann am veränderten Stoffwechsel der Zellen liegen, die Sie verwenden ( schließlich handelt es sich hier laut ATCC um ein Endothelioma).


    2. Schauen Sie sich bitte einmal das Dokument an, welches ich an diese Email gehängt habe: Dieses Dokument zeigt sehr schön, wie das Gelsystem das Laufverhalten beeinflußen kann.

    3. Eine Abweichung von bis zu 10% von der erwarteten Groesse kann durchaus als normal angesehen werden.

    4. Es gibt zwei Formen dieses Proteins, die lösliche und die zelluläre Form. Die lösliche From ist ca 10 kDa leichter als die zellulare (138 kDa vs 148 kDA).

    5. Da Sie leider mit einem System arbeiten (Odyssey), ist es schwierig Veränderungsvorschläge zu Ihrem Blot zu machen. Es würde mich allerdings interessieren, ob Sie via Chemolumineszenz oder Fluoreszenz detektieren.

    6. Um auszuschließen, dass Sie das falsche Protein detektieren, möchte ich Ihnen ein Maushirn Lysat als Positiv- bzw Vergleichsprobe kostenlos zusenden. Bitte lassen Sie mich wissen, ob Sie damit einverstanden sind.

    Read More

    Question
    Answer

    Vielen Dank für Ihren Anruf.

    Es tut mir leid zu hören, dass Sie Probleme mit diesen Antikörpern haben.

    Ich habe unseren Fragebogen als Word-Dokument an diese E-Mail angehängt. Durch das Ausfüllen des Fragebogens erhalten wir alle nötigen Informationen über Ihre Proben und Ihr Protokoll. Sobald Sie dieses Formular an uns zurückgeschickt haben, werden wir uns Ihr Protokoll ansehen und möglichst Veränderungsvorschläge machen, die Ihre Ergebnisse verbessern werden. Falls sich herausstellt, dass der Antikörper nicht so funktioniert, wie auf dem Datenblatt beschrieben und er innerhalb der letzten 180 Tage gekauft wurde, werden wir Ihnen gerne einen Ersatz oder eine Gutschrift schicken.


    Ich freue mich, bald wieder von Ihnen zu hören.

    Read More

    Answer

    Thank you for contacting us. The immunogen of ab75762 shares 57% identity with the testis-specific isoform of Angiotensin Converting Enzyme 1. Thus, I would not predict that it would recognize this isoform. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

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