Overview

  • Product name

    Anti-Angiotensin Converting Enzyme 2 antibody
    See all Angiotensin Converting Enzyme 2 primary antibodies
  • Description

    Rabbit polyclonal to Angiotensin Converting Enzyme 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Angiotensin Converting Enzyme 2 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q9BYF1

  • Positive control

    • Human kidney, testis and breast carcinoma tissue; HL60 cell lysate; HL60 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab189168 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 93 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.
  • Tissue specificity

    Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system.
  • Sequence similarities

    Belongs to the peptidase M2 family.
  • Post-translational
    modifications

    N-glycosylation on Asn-90 may limit SARS infectivity.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACE 2 antibody
    • ACE related carboxypeptidase antibody
    • ACE-related carboxypeptidase antibody
    • ACE2 antibody
    • ACE2_HUMAN antibody
    • ACEH antibody
    • Angiotensin converting enzyme 2 antibody
    • Angiotensin converting enzyme homolog antibody
    • Angiotensin converting enzyme like protein antibody
    • Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2 antibody
    • Angiotensin I converting enzyme 2 antibody
    • Angiotensin-converting enzyme homolog antibody
    • DKFZP434A014 antibody
    • EC 3.4.17 antibody
    • metalloprotease MPROT 15 antibody
    • Metalloprotease MPROT15 antibody
    • OTTHUMP00000022963 antibody
    • Processed angiotensin-converting enzyme 2 antibody
    see all

Images

  • Anti-Angiotensin Converting Enzyme 2 antibody (ab189168) at 1/1000 dilution + HL60 cell lysate at 35 µg

    Predicted band size: 93 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling Angiotensin Converting Enzyme 2 with ab189168 at 5 µg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue, labeling Angiotensin Converting Enzyme 2 with ab189168 at 5 µg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue, labeling Angiotensin Converting Enzyme 2 with ab189168 at 5 µg/ml.

  • Flow cytometry analysis of Angiotensin Converting Enzyme 2 expression in HL60 cells cells using ab189168 at 1/10 dilution (green) compared to a negative control cell (blue). A FITC-conjugated goat anti-rabbit IgG was used as the secondary antibody.

References

ab189168 has not yet been referenced specifically in any publications.

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