Recombinant
RabMAb

Recombinant Anti-Angiotensin Converting Enzyme 2 antibody [EPR4435(2)] (ab108252)

Overview

  • Product name

    Anti-Angiotensin Converting Enzyme 2 antibody [EPR4435(2)]
    See all Angiotensin Converting Enzyme 2 primary antibodies
  • Description

    Rabbit monoclonal [EPR4435(2)] to Angiotensin Converting Enzyme 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Angiotensin Converting Enzyme 2 aa 200-300 (extracellular). The exact sequence is proprietary.
    (Peptide available as ab198988)

  • Positive control

    • Fetal kidney and Human testis lysate; Human kidney tissue; Human testis tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab108252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 92 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.
    • Tissue specificity

      Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system.
    • Sequence similarities

      Belongs to the peptidase M2 family.
    • Post-translational
      modifications

      N-glycosylation on Asn-90 may limit SARS infectivity.
    • Cellular localization

      Secreted and Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • ACE 2 antibody
      • ACE related carboxypeptidase antibody
      • ACE-related carboxypeptidase antibody
      • ACE2 antibody
      • ACE2_HUMAN antibody
      • ACEH antibody
      • Angiotensin converting enzyme 2 antibody
      • Angiotensin converting enzyme homolog antibody
      • Angiotensin converting enzyme like protein antibody
      • Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2 antibody
      • Angiotensin I converting enzyme 2 antibody
      • Angiotensin-converting enzyme homolog antibody
      • DKFZP434A014 antibody
      • EC 3.4.17 antibody
      • metalloprotease MPROT 15 antibody
      • Metalloprotease MPROT15 antibody
      • OTTHUMP00000022963 antibody
      • Processed angiotensin-converting enzyme 2 antibody
      see all

    Images

    • All lanes : Anti-Angiotensin Converting Enzyme 2 antibody [EPR4435(2)] (ab108252) at 1/1000 dilution

      Lane 1 : Fetal kidney lysate
      Lane 2 : Human testis lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 92 kDa

    • ab108252, at 1/100 dilution, staining Angiotensin Converting Enzyme 2 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • ab108252, at 1/100 dilution, staining Angiotensin Converting Enzyme 2 in paraffin-embedded Human testis tissue by Immunohistochemistry

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Poletto Bonetto JH  et al. Impact of early life AT1 blockade on adult cardiac morpho-functional changes and the renin-angiotensin system in a model of neonatal high oxygen-induced cardiomyopathy. Eur J Pharmacol 860:172585 (2019). Read more (PubMed: 31376367) »
    • Tiao MM  et al. Resveratrol ameliorates maternal and post-weaning high-fat diet-induced nonalcoholic fatty liver disease via renin-angiotensin system. Lipids Health Dis 17:178 (2018). Read more (PubMed: 30055626) »
    See all 8 Publications for this product

    Customer reviews and Q&As

    1-6 of 6 Abreviews or Q&A

    Question
    Answer

    Yes of course, thanks for your reply.

    We don't have any specific recommendations for western blotting protocol for ab32212. Although a good starting dilution for western blotting using ab32212 is noted as 1:5000.

    ab52174 is antibody against a phosphorylated epitope so with this antibody it is best to block with BSA for sure instead of milk, as milk contains phosphorylated proteins which may bind the antibody, creating a high background. A recommended starting concentration for this antibody is 1:1000.

    I hope this is helpful. Please contact me again if you have any further questions.

    Read More

    Answer

    Thanks for your email.

    I'm sorry but I don't quite understand your question. What is it that you requested for ab32213 and ab52174?

    Thanks for the additional details.

    Read More

    Answer

    Thank you for your enquiry.

    i have checked and none ofthese 3 antibodies have a specific western blot protocol associated with them, although typically a 1:1000 dilution, or 1ug/ml primaryantibody concentration works well as a starting point for western blotting.

    A good blocking buffer we usually recommend is 5% BSA in TBST.

    A good antibody diluent to use is 1% BSA in TBST.

    Which antibody are you using to stain the rat mesenteric artery extracts?

    Read More

    Question
    Answer

    Thank you for contacting us.

    The link to our list of buffer solutions and their composition is:
    https://www.abcam.com/index.html?pageconfig=resource&rid=11414

    The link to our WB protocol is:
    https://www.abcam.com/index.html?pageconfig=resource&rid=11375
    I have also attached the WB guide to this email for your convenience.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Answer

    Thanks for your email.

    For ab11734 I'd recommend starting with a dilution of 1: 250 and dilute ab87436 to 1:1000. The primary antibody diluent could be TBS with 0.05% Tween 20.

    I hope this is helpful. Please contact me again if you have any further questions.

    Read More

    Answer

    Thank you for your enquiry.

    Here is an antibody that has be used in western blotting and IHC on rat samples for ACE 1:
    https://www.abcam.com/Angiotensin-Converting-Enzyme-1-antibody-9B9-ab77990.html

    Here is an antibody that has be used in western blotting and IHC on rat samples for ACE 2:
    https://www.abcam.com/Angiotensin-Converting-Enzyme-2-antibody-EPR4435-2-ab108252.html

    I hope this is helpful. Please contact me again if you have any further questions.

    Read More

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