Overview

  • Product name
    Anti-Angiotensin I antibody
    See all Angiotensin I primary antibodies
  • Description
    Rabbit polyclonal to Angiotensin I
  • Host species
    Rabbit
  • Specificity
    Specificity of the antisera is confirmed by its ability to bind and precipitate labeled angiotensin I and to be adsorbed by angiotensin. Native angiotensin competes with such binding, while human whole plasma or plasma proteins do not. The antiserum also reacts with angiotensin II (Reactivity in competitive ELISA was shown : 80% to Angiotensin II; 100% to Angiotensin I)
  • Tested applications
    Suitable for: ELISAmore details
    Unsuitable for: IHC
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide:

    DRVYIHPFHL

    conjugated to BSA by a CDI linker, corresponding to N terminal amino acids 34-43 of Human Angiotensin I.

  • General notes
    The enzyme renin cleaves angiotensinogen to produce an inactive prohormone, angiotensin I; the latter is transformed to the active molecule angiotensin II by another enzyme, angiotensin-converting enzyme

Properties

Applications

Our Abpromise guarantee covers the use of ab8051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
  • Application notes
    ELISA: Use at an assay dependent dilution.

    Is unsuitable for IHC.

    Not yet tested in other applications.
    A starting dilution of 1/100 is recommended. Please note that optimal dilutions/concentrations should be determined by the end user.
  • Target

    References

    ab8051 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-6 of 6 Abreviews or Q&A

    Answer

    Reactivity in competitive ELISA was shown: 80% to Angiotensin II 100% to Angiotensin I

    Read More

    Answer

    This product is provided as whole antiserum, and not as a purified immunoglobulin. Thus, there are other proteins present in the sample, especially albumin. The antibody has been tested in ELISA and we guarantee its use in this application. Have you tested this antibody? Are you having problems with it? Please let me know if you are. Please see the specificity statement from the datasheet below: Specificity of the antisera is confirmed by its ability to bind and precipitate labeled angiotensin I and to be adsorbed by angiotensin. Native angiotensin competes with such binding, while human whole plasma or plasma proteins do not. The antiserum also reacts with angiotensin II.

    Read More

    Answer

    First I would like to confirm that AB8051 has been tested and characterized for Immunoassay so it should work for RIA. The recommended compatible secondary antibodies are listed on the datasheet of this antibody, please have a look at it. Ab7019 is Goat polyclonal to Rabbit IgG H&L (agarose) and it is suitable for immunoprecipitation (IP). For details on IP, please click on the “Protocols“ section on the Scientific Support section of our website.

    Read More

    Question
    Answer

    The protein and Ig concentrations of this antibody have not been assessed.

    Read More

    Answer

    Specificity of the antiserum is confirmed by its ability to bind labelled Angiotensin I in precipitation, partition, or adsorption assays. Native Angiotensin I competes with such binding, while human whole plasma or plasma proteins do not. The antiserum reacts also with Angiotensin II. The use of the antiserum is recommended for quantitative immunoassays of Angiotensin I. This product has not been tested on frozen sections.

    Read More

    Question
    Answer

    As far as we are aware, this antibody has not been tested on angiotensinogen.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up