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I recieved antibody ab8051, lot number 145187, from Abcam yesterday (2006-10-17). When I look at it with MS I see very little of the whole antobody and a lot of something 66kDa (albumin?). How can this be a good antibody to use when there is just traces of it compared to albumin? Can one really trust the antibody to still be efficient on capturing Ang I? Please see the inserted spectra.
Asked on Oct 18 2006
This product is provided as whole antiserum, and not as a purified immunoglobulin. Thus, there are other proteins present in the sample, especially albumin. The antibody has been tested in ELISA and we guarantee its use in this application. Have you tested this antibody? Are you having problems with it? Please let me know if you are. Please see the specificity statement from the datasheet below: Specificity of the antisera is confirmed by its ability to bind and precipitate labeled angiotensin I and to be adsorbed by angiotensin. Native angiotensin competes with such binding, while human whole plasma or plasma proteins do not. The antiserum also reacts with angiotensin II.
Answered on Oct 18 2006