Overview

  • Product name

    Angiotensin I ELISA kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    AGT 20 163.6pg/ml 8.3%
    AGT 20 53.2pg/ml 7.3%
    AGT 20 25.1pg/ml 10%
    Inter-assay
    Sample n Mean SD CV%
    AGT 182.2pg/ml 7.9%
    AGT 48.1pg/ml 10%
    AGT 24.4pg/ml 7.6%
  • Sample type

    Serum, Plasma
  • Assay type

    Competitive
  • Sensitivity

    4.3 pg/ml
  • Range

    3.9 pg/ml - 10000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Spike 77.3 38pg/ml - 3286pg/ml

  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Angiotensin I in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Angiotensin I in plasma and serum.

    A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with a solution of LVV Hemorphin 7 conjugated to biotin, followed by a solution of polyclonal antibody to LVV Hemorphin 7. The plate is washed to remove unbound reagents. A solution of streptavidin-HRP conjugate is then added. After further incubation the excess reagents are washed away and TMB substrate is added, which is catalyzed by HRP to generate a yellow color. A stop solution changes this color from yellow to blue, and the intensity of this blue coloration is inversely proportional to the amount of LVV Hemorphin 7 captured in the plate.

     

  • Notes

     

    Analyte

    SequencePercent cross reactivities in the range of 0.1 pM - 500 nM

    Ang(1-12)

    DRVYIHPFHLVI

     

    0.025
    Ang IDRVYIHPFHL100
    Ang(1-9)

    DRVYIHPFH

    0.000
    Ang IIDRVYIHPF0.083
    Ang(1-7)DRVYIHP0.000
    Ang AARVYIHPF0.182
    Ang IIIRVYIHPF0.000
    Ang IVVYIHPF0.000
    LVV-hemorphin 7LVVYPWTQRF0.000
    BradykininRPPGFSPFR0.000
  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab136934 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab136934.

Protocols

References

ab136934 has not yet been referenced specifically in any publications.

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