Key features and details
- Sensitivity: 4.3 pg/ml
- Range: 3.9 pg/ml - 10000 pg/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Product nameAngiotensin I ELISA kit
Intra-assay Sample n Mean SD CV% AGT 20 163.6pg/ml 8.3% AGT 20 53.2pg/ml 7.3% AGT 20 25.1pg/ml 10% Inter-assay Sample n Mean SD CV% AGT 182.2pg/ml 7.9% AGT 48.1pg/ml 10% AGT 24.4pg/ml 7.6%
Sample typeSerum, Plasma
Range3.9 pg/ml - 10000 pg/ml
Sample specific recovery Sample type Average % Range Spike 77.3 38pg/ml - 3286pg/ml
Assay time3h 30m
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s Angiotensin I in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Angiotensin I in plasma and serum.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with a solution of LVV Hemorphin 7 conjugated to biotin, followed by a solution of polyclonal antibody to LVV Hemorphin 7. The plate is washed to remove unbound reagents. A solution of streptavidin-HRP conjugate is then added. After further incubation the excess reagents are washed away and TMB substrate is added, which is catalyzed by HRP to generate a yellow color. A stop solution changes this color from yellow to blue, and the intensity of this blue coloration is inversely proportional to the amount of LVV Hemorphin 7 captured in the plate.
Sequence Percent cross reactivities in the range of 0.1 pM - 500 nM
DRVYIHPFHLVI Ang I DRVYIHPFHL 100 Ang(1-9)
0.000 Ang II DRVYIHPF 0.083 Ang(1-7) DRVYIHP 0.000 Ang A ARVYIHPF 0.182 Ang III RVYIHPF 0.000 Ang IV VYIHPF 0.000 LVV-hemorphin 7 LVVYPWTQRF 0.000 Bradykinin RPPGFSPFR 0.000
Storage instructionsPlease refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 27ml Angiotensin I Biotinylated Conjugate 1 vial Angiotensin I Polyclonal Antibody 1 vial Angiotensin I Standard 1 x 1µg Assay Buffer 16 1 x 30ml Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit HRP- Streptavidin Conjugate 1 x 12.5µg Plate Sealer 2 units Stop Solution 2 1 x 10ml TMB Substrate 1 x 10ml
- Ang I
- Angiotensin I
ab136934 has not yet been referenced specifically in any publications.