Overview

  • Product name

    Anti-Angiotensin II Type 1 Receptor antibody
    See all Angiotensin II Type 1 Receptor primary antibodies
  • Description

    Rabbit polyclonal to Angiotensin II Type 1 Receptor
  • Host species

    Rabbit
  • Specificity

    ab124505 is specific for Angiotensin II Type 1 Receptor (AGTR1) and will not cross-react with AGTR2.
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Angiotensin II Type 1 Receptor (internal sequence).
    Database link: P30556
    (Peptide available as ab176148)

  • Positive control

    • Mouse kidney tissue lysate; Mouse kidney tissue, mouse kidney cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab124505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 41 kDa.Can be blocked with Angiotensin II Type 1 Receptor peptide (ab176148).
IHC-P Use a concentration of 2.5 µg/ml.

Target

  • Function

    Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
  • Tissue specificity

    Liver, lung, adrenal and adrenocortical adenomas.
  • Involvement in disease

    Defects in AGTR1 are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications

    C-terminal Ser or Thr residues may be phosphorylated.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AG2S antibody
    • Agtr 1 antibody
    • Agtr1 antibody
    • AGTR1_HUMAN antibody
    • Agtr1a antibody
    • AGTR1B antibody
    • Ang II antibody
    • Angiotensin II receptor type 1 antibody
    • Angiotensin II type-1 receptor antibody
    • Angiotensin receptor 1 antibody
    • Angiotensin receptor 1B antibody
    • AT 1B antibody
    • AT 1r antibody
    • AT1 antibody
    • At1a antibody
    • AT1AR antibody
    • AT1B antibody
    • AT1BR antibody
    • AT1R antibody
    • AT2R1 antibody
    • AT2R1A antibody
    • AT2R1B antibody
    • HAT1R antibody
    • Type 1 angiotensin II receptor antibody
    • Type 1B angiotensin II receptor antibody
    • Type-1 angiotensin II receptor antibody
    see all

Images

  • Lane 1 : Anti-Angiotensin II Type 1 Receptor antibody (ab124505) at 1 µg/ml
    Lane 2 : Anti-Angiotensin II Type 1 Receptor antibody (ab124505) at 2 µg/ml

    All lanes : Mouse kidney tissue lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 41 kDa

  • Immunofluorescence of AGTR1 in mouse kidney cells using ab124505 at 20 ug/ml.

  • Immunohistochemical analysis of mouse kidney tissue section labelling Angiotensin II Type 1 Receptor with ab124505, at 2.5 µg/ml.

  • ab124505, at 2.5 µg/ml, staining Angiotensin II Type 1 Receptor in paraffin-embedded Mouse kidney tissue by Immunohistochemistry.

References

This product has been referenced in:

  • Wen J  et al. Downregulation of AT2R decreases the responsiveness of BKCa channels to angiotensin II in patients with hypertension. J Mol Cell Cardiol 131:20-28 (2019). Read more (PubMed: 30998981) »
  • Jiang L  et al. Brain Renin-Angiotensin System Blockade Attenuates Methamphetamine-Induced Hyperlocomotion and Neurotoxicity. Neurotherapeutics 15:500-510 (2018). Read more (PubMed: 29464572) »
See all 5 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Flow Cytometry
Sample
Human Cell (HEK293)
Permeabilization
No
Specification
HEK293
Fixation
unfixed

Abcam user community

Verified customer

Submitted Jun 17 2019

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12% Bis-Tris)
Sample
Human Tissue lysate - whole (Human Skeletal Muscle)
Specification
Human Skeletal Muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 17 2013

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