Product nameAnti-Angiotensin II Type 1 Receptor antibody
See all Angiotensin II Type 1 Receptor primary antibodies
DescriptionRabbit polyclonal to Angiotensin II Type 1 Receptor
SpecificityThis antibody recognizes isoforms AT-1A and AT-1B of the Angiotensin II type 1 receptor (AT-1r) in rat. We have received feedback that this antibody may not be specific for the antigen (see PubMed 22843099 on the References page).
Tested applicationsSuitable for: WB, IHC-FoFr, IHC-Pmore details
Species reactivityReacts with: Mouse, Cow, Dog, Pig
Predicted to work with: Rat
PSDNMSSSAKKPASCconjugated to KLH, corresponding to Internal sequence amino acids 341-355 of Angiotensin II Type 1 Receptor AT-1A. Synthetic peptide: SSSAKKSASFFEVE conjugated to KLH, corresponding to Internal sequence amino acids 346-359 of Angiotensin II Type 1 Receptor AT-1B.
- WB: Dog heart whole tissue lysate. IHC-P: Mouse and pig kidney tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 0.81% Sodium chloride, 0.02% Potassium chloride, 0.04% Potassium phosphate, 0.16% Sodium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntisera was affinity purified by passage through two affinity columns, one cross-linked to AT-1A and the other with AT-1B, resulting in specificity to the sequence PSDNMSSSAKKPASCFEVE (341-359).
Our Abpromise guarantee covers the use of ab18801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 41 kDa).|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 19706421|
FunctionReceptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Tissue specificityLiver, lung, adrenal and adrenocortical adenomas.
Involvement in diseaseDefects in AGTR1 are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
modificationsC-terminal Ser or Thr residues may be phosphorylated.
Cellular localizationCell membrane.
- Information by UniProt
- AG2S antibody
- Agtr 1 antibody
- Agtr1 antibody
All lanes : Anti-Angiotensin II Type 1 Receptor antibody (ab18801) at 1/800 dilution
Lane 1 : Dog heart whole tissue lysate at 50 µg
Lane 2 : Dog heart whole tissue lysate at 25 µg
Lane 3 : Dog heart whole tissue lysate at 10 µg
Lane 4 : Dog heart whole tissue lysate at 5 µg
Lane 5 : Dog heart whole tissue lysate at 2 µg
All lanes : HRP conjugated donkey polycloanl antibody
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Additional bands at: 32 kDa (possible non-specific binding)
Exposure time: 2 minutes
ab18801 staining Angiotensin II Type 1 in porcine kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 30 minutes at 20°C; antigen retrieval was by heat mediation with a citrate buffer. Samples were incubated with primary antibody (1/100 in PBS + Triton X-100 + goat serum) for 24 hours at 4°C. An HRP secondary antibody was used.
ab18801 staining Angiotensin II Type 1 Receptor in Mouse kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 30 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in PBS + Triton X100) for 24 hours at 4°C. An undiluted HRP-conjugated anti-rabbit polyclonal was used as the secondary antibody.
This product has been referenced in:
- Chiang JT et al. Eriobotrya japonica ameliorates cardiac hypertrophy in H9c2 cardiomyoblast and in spontaneously hypertensive rats. Environ Toxicol N/A:N/A (2018). Read more (PubMed: 29974613) »
- Zhu Y et al. The Effect and Mechanism of Chinese Herbal Formula Sini Tang in Heart Failure after Myocardial Infarction in Rats. Evid Based Complement Alternat Med 2018:5629342 (2018). Read more (PubMed: 30050591) »