Recombinant
RabMAb

Recombinant Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)

Overview

  • Product name

    Anti-Angiotensin II Type 1 Receptor antibody [EPR3873]
    See all Angiotensin II Type 1 Receptor primary antibodies
  • Description

    Rabbit monoclonal [EPR3873] to Angiotensin II Type 1 Receptor
  • Host species

    Rabbit
  • Specificity

    The immunogen used for this product shares 53% homology with Erbin/LAP2. Cross-reactivity with this protein has not been confirmed experimentally.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Angiotensin II Type 1 Receptor aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P30556

  • Positive control

    • WB: C2C12, PC12, HeLa, and HepG2 whole cell lysate (ab7900).
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 41 kDa).

Target

  • Function

    Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
  • Tissue specificity

    Liver, lung, adrenal and adrenocortical adenomas.
  • Involvement in disease

    Defects in AGTR1 are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications

    C-terminal Ser or Thr residues may be phosphorylated.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AG2S antibody
    • Agtr 1 antibody
    • Agtr1 antibody
    • AGTR1_HUMAN antibody
    • Agtr1a antibody
    • AGTR1B antibody
    • Ang II antibody
    • Angiotensin II receptor type 1 antibody
    • Angiotensin II type-1 receptor antibody
    • Angiotensin receptor 1 antibody
    • Angiotensin receptor 1B antibody
    • AT 1B antibody
    • AT 1r antibody
    • AT1 antibody
    • At1a antibody
    • AT1AR antibody
    • AT1B antibody
    • AT1BR antibody
    • AT1R antibody
    • AT2R1 antibody
    • AT2R1A antibody
    • AT2R1B antibody
    • HAT1R antibody
    • Type 1 angiotensin II receptor antibody
    • Type 1B angiotensin II receptor antibody
    • Type-1 angiotensin II receptor antibody
    see all

Images

  • All lanes : Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734) at 1/2000 dilution (purified)

    Lane 1 : C2C12 cell lysate
    Lane 2 : PC-12 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 41 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734) at 1/2000 dilution (purified) + HT-1080 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 41 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • All lanes : Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734) at 1/1000 dilution (unpurified)

    Lane 1 : C2C12 cell lysate
    Lane 2 : PC12 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 41 kDa

References

This product has been referenced in:

  • Bian J  et al. Limited AT1 Receptor Internalization Is a Novel Mechanism Underlying Sustained Vasoconstriction Induced by AT1 Receptor Autoantibody From Preeclampsia. J Am Heart Assoc 8:e011179 (2019). Read more (PubMed: 30845870) »
  • Su H  et al. LncRNA H19 promotes the proliferation of pulmonary artery smooth muscle cells through AT1R via sponging let-7b in monocrotaline-induced pulmonary arterial hypertension. Respir Res 19:254 (2018). Read more (PubMed: 30547791) »
See all 8 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your interest in ab124734, anti AT-1 receptor. The antibody should be capable of detecting glycosylated receptor. The individual bands shown on the datasheet western blot image may be fully glycosylated receptor, assuming this is how the protein is modified in those cell lines, C2C12, HeLa, PC12 and HepG2, and assuming that the unmodified precursor is present in relatively small amounts compared to the mature protein. The banding pattern seems to be consistent with the blot results for most of the other antibodies we have for the receptor.

We do not have data for tissue lysates. Please let me know if you have any other questions or concerns.

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