Recombinant Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3873] to Angiotensin II Type 1 Receptor
- Suitable for: WB, Indirect ELISA
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Angiotensin II Type 1 Receptor antibody [EPR3873]
See all Angiotensin II Type 1 Receptor primary antibodies -
Description
Rabbit monoclonal [EPR3873] to Angiotensin II Type 1 Receptor -
Host species
Rabbit -
Specificity
The immunogen used for this product shares 53% homology with Erbin/LAP2. Cross-reactivity with this protein has not been confirmed experimentally.
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Tested applications
Suitable for: WB, Indirect ELISAmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Angiotensin II Type 1 Receptor aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P30556 -
Positive control
- WB: C2C12, PC12, HeLa, and HepG2 whole cell lysate (ab7900).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3873 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
|
Indirect ELISA |
Use at an assay dependent concentration.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 41 kDa. |
Indirect ELISA
Use at an assay dependent concentration. |
Target
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Function
Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. -
Tissue specificity
Liver, lung, adrenal and adrenocortical adenomas. -
Involvement in disease
Defects in AGTR1 are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsC-terminal Ser or Thr residues may be phosphorylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 185 Human
- Entrez Gene: 11607 Mouse
- Entrez Gene: 11608 Mouse
- Entrez Gene: 24180 Rat
- Entrez Gene: 81638 Rat
- Omim: 106165 Human
- SwissProt: P30556 Human
- SwissProt: P29754 Mouse
see all -
Alternative names
- AG2S antibody
- Agtr 1 antibody
- Agtr1 antibody
see all
Images
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Lane 1 : Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734) at 1/1000 dilution
Lane 2 : LIF-tag at 1/10000 dilution
All lanes : LIF tagged Recombinant Human AGTR1 protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: Lane 1: 40 seconds - Lane 2: 80 seconds.
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All lanes : Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734) at 1/2000 dilution (purified)
Lane 1 : C2C12 cell lysate
Lane 2 : PC-12 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 41 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
ELISA analysis of Human Angiotensin II Type 1 Receptor recombinant protein at 250 ng/mL with ab124734. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
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Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734) at 1/2000 dilution (purified) + HT-1080 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 41 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
All lanes : Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734) at 1/1000 dilution (unpurified)
Lane 1 : C2C12 cell lysate
Lane 2 : PC12 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 41 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (35)
ab124734 has been referenced in 35 publications.
- Li RJ et al. Qing Fei Hua Xian Decoction ameliorates bleomycin-induced pulmonary fibrosis by suppressing oxidative stress through balancing ACE-AngII-AT1R/ACE2-Ang-(1-7)-Mas axis. Iran J Basic Med Sci 26:107-113 (2023). PubMed: 36594067
- Zhu Z et al. Homocysteine promotes migration of adventitial fibroblasts via angiotensin II type 1 receptor activation to aggravate atherosclerosis. Gen Physiol Biophys 41:535-547 (2022). PubMed: 36454114
- Kim HY et al. Oryeongsan (Wulingsan) ameliorates impaired ANP secretion of atria from spontaneously hypertensive rats. Biomed Pharmacother 146:112433 (2022). PubMed: 35062052
- Liu X et al. Pre-miRNA Hsa-Let-7a-2: a Novel Intracellular Partner of Angiotensin II Type 2 Receptor Negatively Regulating its Signals. Int J Biol Sci 18:3237-3250 (2022). PubMed: 35637969
- Nguyen BL et al. Alterations in renin-angiotensin receptors are not responsible for exercise preconditioning of skeletal muscle fibers. Sports Med Health Sci 3:148-156 (2021). PubMed: 35784524