Overview

  • Product name
    Anti-Angiotensin II Type 2 Receptor antibody
    See all Angiotensin II Type 2 Receptor primary antibodies
  • Description
    Rabbit polyclonal to Angiotensin II Type 2 Receptor
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, Electron Microscopy, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Sheep, Rabbit
  • Immunogen

    Synthetic peptide corresponding to Rat Angiotensin II Type 2 Receptor aa 349-363 (extracellular).
    Sequence:

    CRKSSSLREMETFVS

  • Positive control
    • IHC-P: Human brain tissue. ICC/IF: PC12 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab19134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
Electron Microscopy Use at an assay dependent concentration. PubMed: 19539732
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    Receptor for angiotensin II. Cooperates with MTUS1 to inhibit ERK2 activation and cell proliferation.
  • Tissue specificity
    In adult, highly expressed in myometrium with lower levels in adrenal gland and fallopian tube. Very highly expressed in fetal kidney and intestine.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AGTR 2 antibody
    • Agtr2 antibody
    • AGTR2_HUMAN antibody
    • angiotensin II receptor type 2 antibody
    • Angiotensin II type-2 receptor antibody
    • Angiotensin receptor 2 antibody
    • AT 2 antibody
    • AT2 antibody
    • ATGR 2 antibody
    • ATGR2 antibody
    • MRX 88 antibody
    • MRX88 antibody
    • Type 2 angiotensin II receptor antibody
    • Type-2 angiotensin II receptor antibody
    see all

Images

  • ab19134 at 1/2000 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and blocked with serum prior to incubation with the antibody for 18 hours. An HRP conjugated horse polyclonal antibody was used as the secondary.

    See Abreview

  • ICC/IF image of ab19134 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19134, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Almalki SG & Agrawal DK ERK signaling is required for VEGF-A/VEGFR2-induced differentiation of porcine adipose-derived mesenchymal stem cells into endothelial cells. Stem Cell Res Ther 8:113 (2017). WB ; Pig . Read more (PubMed: 28499402) »
  • Delforce SJ  et al. Expression of renin-angiotensin system (RAS) components in endometrial cancer. Endocr Connect 6:9-19 (2017). Read more (PubMed: 27956412) »
See all 35 Publications for this product

Customer reviews and Q&As

1-10 of 18 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Bos taurus Tissue sections (ovary follicle)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate and citric acid buffer
Permeabilization
No
Specification
ovary follicle
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Nov 17 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated
Sample
Mouse Tissue sections (Aorta)
Specification
Aorta
Permeabilization
No
Fixative
Formalin 10%

Abcam user community

Verified customer

Submitted Jul 21 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (epididymis)
Specification
epididymis
Fixative
bouin's solution
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 12 2013

Answer

Thank you for contacting us.

HEK293 lysate, Hela cell lysate, and human testis lysate have all been shown to have good results in WB for Angiotensin II type 2 Receptor. We do have these lysates available in our catalog as ab7902, ab150035, and ab30257.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for contacting us.
The credit note was issued and its ID is xxxxx.
I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:
(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.
(3) A full refund can be offered where no other invoices are outstanding.
Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.
To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.
The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.
I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Brain)
Loading amount
30 µg
Specification
Brain
Treatment
Diluted 1:2 in sample buffer treated with ß-mercaptoethanol
Gel Running Conditions
Reduced Non-Denaturing (Native) (4-20% Tris-HCl pre-cast gels)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

Miss. Laura Palmer

Verified customer

Submitted Nov 19 2012

Answer

Thank you for contacting us. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab19134. The trouble with this protein is that it is a glycoprotein and it is a multi-pass membrane protein. As a result, these have trouble resolving in gels.

Adding glycerol and not boiling the samples prevents the serious smudging of samples that happens when hydrophobic proteins are run on a traditional polyacrylamide gel. For example AGTR2, which has7 transmembrane domains, would appear as a smear on a traditional gel with heated samples. The band shouldtighten up a bit with reduced smudging on a gel containing glycerol when the samples are not heated.

Add glycerol to a final concentration of 10% in the resolving portion of the gel-not the stacking portion. Also, heat samplesat 70°C for 5-10 minutes instead of boiling.

You may want to try diluting the primary and secondary antibodies more. Be sure to incubate the primary antibody overnight, if you're not already, and include the blocking buffer in with the antibody. You could also try switching blocking buffer. It seems like customers have had success with 5% BSA with this antibody. Also be sure that you have tween in with your wash buffer, whether it is PBS or TBS.

In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

I hope this information is helpful, and I thank you for your cooperation.

Read More

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement:

Order number: ######

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Answer

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will provide us with vital information for our monitoring of product quality. I would like to reassure you that this antibody is tested and covered by our guarantee forWB and Mouse, Rat, Cow andDog samples.

I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. Reviewing the details, as the othervial has worked well, I am sorry it will be difficult to provide further tips to provide on this occasion to help improve the results. I can suggest you have regrettably received a bad vial.

If you have purchased within the guarantee period of 6 months and used the antibody on a tested and guaranteed species, I am pleased to offer you a free of charge replacement or credit note in compensation. In order to proceed with this, and also to provide some further information for quality monitoring purposes, I would appreciate if you could confirm the following:

1. The order number and date of purchase.

2. Which species was the sample from and from which tissue type or cell line?

3. Please confirm which concentration has the antibody been tried at. Has the incubation been tried overnight at 4oC?

4. What were the storage conditions for the antibody?

Thank you for your time and cooperation. I look forward to hearing from you with the requested information and details of how you would like to proceed.

Read More

Answer

Thank you for your enquiry. I can confirm the following concentrations: ab18801 Angiotensin II Type 1 Receptor   2.1 mg/ml ab19134 Angiotensin II Type 2 Receptor 1.6 mg/ml as stated on the datasheet I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

Read More

1-10 of 18 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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