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Anti-Angiotensin II Type 2 Receptor antibody (ab19134)

  • Datasheet
  • SDS
Reviews (10)Q&A (8)References (41)

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Immunocytochemistry/ Immunofluorescence - Anti-Angiotensin II Type 2 Receptor antibody (ab19134)

    Key features and details

    • Rabbit polyclonal to Angiotensin II Type 2 Receptor
    • Suitable for: ICC/IF
    • Reacts with: Rat
    • Isotype: IgG

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    Anti-Angiotensin II Type 1 Receptor antibody (ab124505)
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    Recombinant Human Angiotensin II Type 2 Receptor protein (ab157871)
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    Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675)

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    Overview

    • Product name

      Anti-Angiotensin II Type 2 Receptor antibody
      See all Angiotensin II Type 2 Receptor primary antibodies
    • Description

      Rabbit polyclonal to Angiotensin II Type 2 Receptor
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      ICC/IF
      Rat
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to Rat Angiotensin II Type 2 Receptor aa 349-363 (intracellular).
      Sequence:

      CRKSSSLREMETFVS

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Constituents: PBS, 0.02% Potassium chloride, 0.04% Potassium phosphate, 0.81% Sodium chloride, 0.16% Sodium phosphate
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Signaling Pathway
      • Lipid Signaling
      • PLC
      • Signal Transduction
      • Signaling Pathway
      • G Protein Signaling
      • GPCR
      • Cardiovascular
      • Atherosclerosis
      • Apoptosis
      • Cardiovascular
      • Blood
      • Blood Pressure regulation

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Angiotensin II Type 2 Receptor protein (ab157871)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab19134 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    ICC/IF
    Rat
    All applications
    Sheep
    Rabbit
    Application Abreviews Notes
    ICC/IF
    Use a concentration of 1 µg/ml.
    Notes
    ICC/IF
    Use a concentration of 1 µg/ml.

    Target

    • Function

      Receptor for angiotensin II. Cooperates with MTUS1 to inhibit ERK2 activation and cell proliferation.
    • Tissue specificity

      In adult, highly expressed in myometrium with lower levels in adrenal gland and fallopian tube. Very highly expressed in fetal kidney and intestine.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession P50052 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 24182 Rat
      • SwissProt: P35351 Rat
      • Unigene: 162367 Rat
      • Alternative names

        • AGTR 2 antibody
        • Agtr2 antibody
        • AGTR2_HUMAN antibody
        • angiotensin II receptor type 2 antibody
        • Angiotensin II type-2 receptor antibody
        • Angiotensin receptor 2 antibody
        • AT 2 antibody
        • AT2 antibody
        • ATGR 2 antibody
        • ATGR2 antibody
        • MRX 88 antibody
        • MRX88 antibody
        • Type 2 angiotensin II receptor antibody
        • Type-2 angiotensin II receptor antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-Angiotensin II Type 2 Receptor antibody (ab19134)
        Immunocytochemistry/ Immunofluorescence - Anti-Angiotensin II Type 2 Receptor antibody (ab19134)
        ICC/IF image of ab19134 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19134, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

      Protocols

      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (41)

      Publishing research using ab19134? Please let us know so that we can cite the reference in this datasheet.

      ab19134 has been referenced in 41 publications.

      • Bhat SA  et al. AT2R Activation Prevents Microglia Pro-inflammatory Activation in a NOX-Dependent Manner: Inhibition of PKC Activation and p47phox Phosphorylation by PP2A. Mol Neurobiol 56:3005-3023 (2019). PubMed: 30076526
      • Beitia M  et al. Altered expression of renin-angiotensin system receptors throughout colorectal adenoma-adenocarcinoma sequence. Int J Med Sci 16:813-821 (2019). PubMed: 31337954
      • Patel SN  et al. Angiotensin II Type 2 Receptor and Receptor Mas Are Colocalized and Functionally Interdependent in Obese Zucker Rat Kidney. Hypertension 70:831-838 (2017). PubMed: 28827476
      • Delforce SJ  et al. Expression of renin-angiotensin system (RAS) components in endometrial cancer. Endocr Connect 6:9-19 (2017). PubMed: 27956412
      • Khan N  et al. Attenuation of the Infiltration of Angiotensin II Expressing CD3+T-Cells and the Modulation of Nerve Growth Factor in Lumbar Dorsal Root Ganglia - A Possible Mechanism Underpinning Analgesia Produced by EMA300, An Angiotensin II Type 2 (AT2) Receptor Antagonist. Front Mol Neurosci 10:389 (2017). PubMed: 29200998
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-10 of 18 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Angiotensin II Type 2 Receptor antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Bos taurus Tissue sections (ovary follicle)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: sodium citrate and citric acid buffer
      Permeabilization
      No
      Specification
      ovary follicle
      Blocking step
      BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Nov 17 2015

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Angiotensin II Type 2 Receptor antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
      Antigen retrieval step
      Heat mediated
      Sample
      Mouse Tissue sections (Aorta)
      Specification
      Aorta
      Permeabilization
      No
      Fixative
      Formalin 10%
      Read More

      Abcam user community

      Verified customer

      Submitted Jul 21 2014

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Angiotensin II Type 2 Receptor antibody

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (epididymis)
      Specification
      epididymis
      Fixative
      bouin's solution
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: citrate buffer
      Permeabilization
      No
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Apr 12 2013

      Question


      Inquiry: Hi, I would like to ask you what would be the best positive control for this antibody in case of Western and MRNA expression experiments?

      Read More

      Abcam community

      Verified customer

      Asked on Mar 05 2013

      Answer

      Thank you for contacting us.

      HEK293 lysate, Hela cell lysate, and human testis lysate have all been shown to have good results in WB for Angiotensin II type 2 Receptor. We do have these lysates available in our catalog as ab7902, ab150035, and ab30257.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      Answered on Mar 05 2013

      Question

      The customer decided to receive a credit.

      Please issue a credit note for Zotal.

      Thanks in advance for your cooperation.

      Best Regards,

      Read More

      Abcam community

      Verified customer

      Asked on Nov 29 2012

      Answer

      Thank you for contacting us.
      The credit note was issued and its ID is xxxxx.
      I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:
      (1) Redeemed against the original invoice if this hasn't already been paid.
      (2) Held on the account for use against a future order.
      (3) A full refund can be offered where no other invoices are outstanding.
      Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.
      To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.
      The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.
      I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

      Read More

      Abcam Scientific Support

      Answered on Nov 29 2012

      Western blot abreview for Anti-Angiotensin II Type 2 Receptor antibody

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Tissue lysate - whole (Brain)
      Loading amount
      30 µg
      Specification
      Brain
      Treatment
      Diluted 1:2 in sample buffer treated with ß-mercaptoethanol
      Gel Running Conditions
      Reduced Non-Denaturing (Native) (4-20% Tris-HCl pre-cast gels)
      Blocking step
      Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
      Read More

      Miss. Laura Palmer

      Verified customer

      Submitted Nov 19 2012

      Question

      Dear Technical Service, I am trying to find an antibody for angiotensin 2 receptor type 2 and recently have tried two of your antibodies.
      I transfected HEK cells with AGTR2 and ran the lysates on a western blot with abcam #19134. I got no band in the nontransfected cells
      and lots of bands in the transfected cells. I tried running 30 ug and 40 ug. I am including the actin from the same lysates as a reference.
      I would appreciate any insight into the multiple smear of bands. Thank you. Jean

      Read More

      Abcam community

      Verified customer

      Asked on Aug 03 2012

      Answer

      Thank you for contacting us. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

      Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab19134. The trouble with this protein is that it is a glycoprotein and it is a multi-pass membrane protein. As a result, these have trouble resolving in gels.

      Adding glycerol and not boiling the samples prevents the serious smudging of samples that happens when hydrophobic proteins are run on a traditional polyacrylamide gel. For example AGTR2, which has7 transmembrane domains, would appear as a smear on a traditional gel with heated samples. The band shouldtighten up a bit with reduced smudging on a gel containing glycerol when the samples are not heated.

      Add glycerol to a final concentration of 10% in the resolving portion of the gel-not the stacking portion. Also, heat samplesat 70°C for 5-10 minutes instead of boiling.

      You may want to try diluting the primary and secondary antibodies more. Be sure to incubate the primary antibody overnight, if you're not already, and include the blocking buffer in with the antibody. You could also try switching blocking buffer. It seems like customers have had success with 5% BSA with this antibody. Also be sure that you have tween in with your wash buffer, whether it is PBS or TBS.

      In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

      I hope this information is helpful, and I thank you for your cooperation.

      Read More

      Abcam Scientific Support

      Answered on Aug 03 2012

      Question

      Thank you for your reply. Before I give all the details that you requested I just wanted to say that abcam is my first choice in terms of antibodies and I have not been disappointed so far.

      1. The purchase order is: ######

      2. Tissue type: Rat kidney cortex

      3. Conditions: I have tried a range – 1to1000, 1to2000, 1to4000, 1to8000 dilutions, and I obtained linearity with 1to2000 dilution and 40ug, 60ug and 80ug of protein. I am running western with 60ug of protein and 1to2000 dilution of primary antibody. The incubation with primary antibody was indeed carried out overnight in 4oC.

      4. Storage: On the delivery day I spin down (in 4oC) and aliquoted antibody to 5ul. Eppendorf tubes were additionally wrapt with paraffin film to prevent any losses. Tubes were kept at -20oC.

      I would be grateful for another tube of antibody as I have still got more samples to run. If you need any additional information please let me know.

      With best regards,

      Read More

      Abcam community

      Verified customer

      Asked on Mar 14 2012

      Answer

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

      I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement:

      Order number: ######

      To check the status of the order please contact our Customer Service team and reference this number.

      Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

      I wish you the best of luck with your research.

      Read More

      Abcam Scientific Support

      Answered on Mar 14 2012

      Question

      Dear Madam/Sir,

      I have been using your ab19134 anti- AT2R (rabbit polyclonal primary antibody) for quite a long time. It worked beautifully until last week.

      I ordered from you in the end of last year 2 tubes (2x50ug) of ab19134, both lot: GR-55294-1. The antibody was aliquoted to the required amount and stored as advised. Until recently I have been using aliquots from one tube and it was fine. This week I used antibody aliquots from the second tube and my western did not work properly. Western blot: I am using Amersham fluorescent double staining. On the same membrane, at the same time I scan for bands of AT2R and Histone. As you can see on the membrane scanned on 06-03-12 histone is fine (Please see attached images). The membrane from 01-03-12 is the good one but on the 06-03-12 I can barely see the band for AT2R. I did not change anything but the primary antibody. Since I treated both of the tubes in the same way from the arrival I suspect that the problem occurred before ab19134 was shipped. Therefore, would it be possible to get a refund or another 50ug of ab19134?

      Regards,

      Read More

      Abcam community

      Verified customer

      Asked on Mar 12 2012

      Answer

      Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

      The details you have kindly provided will provide us with vital information for our monitoring of product quality. I would like to reassure you that this antibody is tested and covered by our guarantee forWB and Mouse, Rat, Cow andDog samples.

      I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. Reviewing the details, as the othervial has worked well, I am sorry it will be difficult to provide further tips to provide on this occasion to help improve the results. I can suggest you have regrettably received a bad vial.

      If you have purchased within the guarantee period of 6 months and used the antibody on a tested and guaranteed species, I am pleased to offer you a free of charge replacement or credit note in compensation. In order to proceed with this, and also to provide some further information for quality monitoring purposes, I would appreciate if you could confirm the following:

      1. The order number and date of purchase.

      2. Which species was the sample from and from which tissue type or cell line?

      3. Please confirm which concentration has the antibody been tried at. Has the incubation been tried overnight at 4oC?

      4. What were the storage conditions for the antibody?

      Thank you for your time and cooperation. I look forward to hearing from you with the requested information and details of how you would like to proceed.

      Read More

      Abcam Scientific Support

      Answered on Mar 12 2012

      Question

       Dear Madam/Sir, I ordered ab19134 from you at November 2010 (last year), the order number is : ########. Could you find out for me what concentration had this batch of ab19134.   Additionally I would like to know what concentration have ab18801, which I ordered at August 2011 (this year), the order number is : ########.   Thank you for your help,      

      Read More

      Abcam community

      Verified customer

      Asked on Nov 28 2011

      Answer

      Thank you for your enquiry. I can confirm the following concentrations: ab18801 Angiotensin II Type 1 Receptor   2.1 mg/ml ab19134 Angiotensin II Type 2 Receptor 1.6 mg/ml as stated on the datasheet I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

      Read More

      Abcam Scientific Support

      Answered on Nov 28 2011

      1-10 of 18 Abreviews or Q&A

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