Key features and details
- Rabbit polyclonal to Angiotensin II Type 2 Receptor
- Suitable for: ICC/IF
- Reacts with: Rat
- Isotype: IgG
Product nameAnti-Angiotensin II Type 2 Receptor antibody
See all Angiotensin II Type 2 Receptor primary antibodies
DescriptionRabbit polyclonal to Angiotensin II Type 2 Receptor
Tested Applications & Species
Application Species ICC/IFRat
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 0.02% Potassium chloride, 0.04% Potassium phosphate, 0.81% Sodium chloride, 0.16% Sodium phosphate
Concentration information loading...
PurityProtein A purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab19134 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Use a concentration of 1 µg/ml.
Use a concentration of 1 µg/ml.
FunctionReceptor for angiotensin II. Cooperates with MTUS1 to inhibit ERK2 activation and cell proliferation.
Tissue specificityIn adult, highly expressed in myometrium with lower levels in adrenal gland and fallopian tube. Very highly expressed in fetal kidney and intestine.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- AGTR 2 antibody
- Agtr2 antibody
- AGTR2_HUMAN antibody
ICC/IF image of ab19134 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19134, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab19134 has been referenced in 41 publications.
- Bhat SA et al. AT2R Activation Prevents Microglia Pro-inflammatory Activation in a NOX-Dependent Manner: Inhibition of PKC Activation and p47phox Phosphorylation by PP2A. Mol Neurobiol 56:3005-3023 (2019). PubMed: 30076526
- Beitia M et al. Altered expression of renin-angiotensin system receptors throughout colorectal adenoma-adenocarcinoma sequence. Int J Med Sci 16:813-821 (2019). PubMed: 31337954
- Patel SN et al. Angiotensin II Type 2 Receptor and Receptor Mas Are Colocalized and Functionally Interdependent in Obese Zucker Rat Kidney. Hypertension 70:831-838 (2017). PubMed: 28827476
- Delforce SJ et al. Expression of renin-angiotensin system (RAS) components in endometrial cancer. Endocr Connect 6:9-19 (2017). PubMed: 27956412
- Khan N et al. Attenuation of the Infiltration of Angiotensin II Expressing CD3+T-Cells and the Modulation of Nerve Growth Factor in Lumbar Dorsal Root Ganglia - A Possible Mechanism Underpinning Analgesia Produced by EMA300, An Angiotensin II Type 2 (AT2) Receptor Antagonist. Front Mol Neurosci 10:389 (2017). PubMed: 29200998