Anti-Angiotensin II Type 2 Receptor antibody (ab19134)

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HEK293 lysate, Hela cell lysate, and human testis lysate have all been shown to have good results in WB for Angiotensin II type 2 Receptor. We do have these lysates available in our catalog as ab7902, ab150035, and ab30257.

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The credit note was issued and its ID is xxxxx.
I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:
(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.
(3) A full refund can be offered where no other invoices are outstanding.
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I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

Thank you for contacting us. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab19134. The trouble with this protein is that it is a glycoprotein and it is a multi-pass membrane protein. As a result, these have trouble resolving in gels.

Adding glycerol and not boiling the samples prevents the serious smudging of samples that happens when hydrophobic proteins are run on a traditional polyacrylamide gel. For example AGTR2, which has7 transmembrane domains, would appear as a smear on a traditional gel with heated samples. The band shouldtighten up a bit with reduced smudging on a gel containing glycerol when the samples are not heated.

Add glycerol to a final concentration of 10% in the resolving portion of the gel-not the stacking portion. Also, heat samplesat 70°C for 5-10 minutes instead of boiling.

You may want to try diluting the primary and secondary antibodies more. Be sure to incubate the primary antibody overnight, if you're not already, and include the blocking buffer in with the antibody. You could also try switching blocking buffer. It seems like customers have had success with 5% BSA with this antibody. Also be sure that you have tween in with your wash buffer, whether it is PBS or TBS.

In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

I hope this information is helpful, and I thank you for your cooperation.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement:

Order number: ######

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will provide us with vital information for our monitoring of product quality. I would like to reassure you that this antibody is tested and covered by our guarantee forWB and Mouse, Rat, Cow andDog samples.

I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. Reviewing the details, as the othervial has worked well, I am sorry it will be difficult to provide further tips to provide on this occasion to help improve the results. I can suggest you have regrettably received a bad vial.

If you have purchased within the guarantee period of 6 months and used the antibody on a tested and guaranteed species, I am pleased to offer you a free of charge replacement or credit note in compensation. In order to proceed with this, and also to provide some further information for quality monitoring purposes, I would appreciate if you could confirm the following:

1. The order number and date of purchase.

2. Which species was the sample from and from which tissue type or cell line?

3. Please confirm which concentration has the antibody been tried at. Has the incubation been tried overnight at 4oC?

4. What were the storage conditions for the antibody?

Thank you for your time and cooperation. I look forward to hearing from you with the requested information and details of how you would like to proceed.

Thank you for your enquiry. I can confirm the following concentrations: ab18801 Angiotensin II Type 1 Receptor   2.1 mg/ml ab19134 Angiotensin II Type 2 Receptor 1.6 mg/ml as stated on the datasheet I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.