Anti-Angiotensin II Type 2 Receptor antibody (ab19134)
- Datasheet
- Specific References (34)
- Protocols
Overview
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Product nameAnti-Angiotensin II Type 2 Receptor antibody
See all Angiotensin II Type 2 Receptor primary antibodies -
DescriptionRabbit polyclonal to Angiotensin II Type 2 Receptor
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Host speciesRabbit
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SpecificityWe have received mixed feedback regarding the use of this antibody in western blot. There are abReviews and specific references that reported the successful use of this antibody in western blot, but the lab's testing found this antibody to be unsuitable for western blot; thus, we have removed western blot as a guaranteed application. We welcome any additional feedback from researchers who have used this antibody in western blot.
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Tested applicationsSuitable for: ICC/IF, Electron Microscopy, IHC-Fr, IHC-Pmore details
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Species reactivityReacts with: Rat, Human
Predicted to work with: Sheep, Rabbit
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Immunogen
Synthetic peptide:
CRKSSSLREMETFVS
conjugated to KLH, corresponding to amino acids 349-363 of Rat Angiotensin II Type 2 Receptor.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Control Peptide
Applications
Our Abpromise guarantee covers the use of ab19134 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. | |
| Electron Microscopy | Use at an assay dependent concentration. PubMed: 19539732 | |
| IHC-Fr | Use at an assay dependent concentration. | |
| IHC-P | 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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FunctionReceptor for angiotensin II. Cooperates with MTUS1 to inhibit ERK2 activation and cell proliferation.
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Tissue specificityIn adult, highly expressed in myometrium with lower levels in adrenal gland and fallopian tube. Very highly expressed in fetal kidney and intestine.
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Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
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Cellular localizationCell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 186 Human
- Entrez Gene: 24182 Rat
- Omim: 300034 Human
- SwissProt: P50052 Human
- SwissProt: P35351 Rat
- Unigene: 405348 Human
- Unigene: 162367 Rat
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Alternative names
- AGTR 2 antibody
- Agtr2 antibody
- AGTR2_HUMAN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin II Type 2 Receptor antibody (ab19134)This image is courtesy of an Abreview submitted by Miss Laura Palmerab19134 at 1/2000 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and blocked with serum prior to incubation with the antibody for 18 hours. An HRP conjugated horse polyclonal antibody was used as the secondary. -
Immunocytochemistry/ Immunofluorescence - Anti-Angiotensin II Type 2 Receptor antibody (ab19134)ICC/IF image of ab19134 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19134, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References
This product has been referenced in:
- Almalki SG & Agrawal DK ERK signaling is required for VEGF-A/VEGFR2-induced differentiation of porcine adipose-derived mesenchymal stem cells into endothelial cells. Stem Cell Res Ther 8:113 (2017). WB ; Pig . Read more (PubMed: 28499402) »
- Delforce SJ et al. Expression of renin-angiotensin system (RAS) components in endometrial cancer. Endocr Connect 6:9-19 (2017). Read more (PubMed: 27956412) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"