Overview

  • Product name

    Anti-Angiotensin II Type 2 Receptor antibody
    See all Angiotensin II Type 2 Receptor primary antibodies
  • Description

    Rabbit polyclonal to Angiotensin II Type 2 Receptor
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, Electron Microscopy, IHC-Fr, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Sheep, Rabbit
  • Immunogen

    Synthetic peptide corresponding to Rat Angiotensin II Type 2 Receptor aa 349-363 (extracellular).
    Sequence:

    CRKSSSLREMETFVS

  • Positive control

    • IHC-P: Human brain tissue. ICC/IF: PC12 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab19134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
Electron Microscopy Use at an assay dependent concentration. PubMed: 19539732
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Receptor for angiotensin II. Cooperates with MTUS1 to inhibit ERK2 activation and cell proliferation.
  • Tissue specificity

    In adult, highly expressed in myometrium with lower levels in adrenal gland and fallopian tube. Very highly expressed in fetal kidney and intestine.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AGTR 2 antibody
    • Agtr2 antibody
    • AGTR2_HUMAN antibody
    • angiotensin II receptor type 2 antibody
    • Angiotensin II type-2 receptor antibody
    • Angiotensin receptor 2 antibody
    • AT 2 antibody
    • AT2 antibody
    • ATGR 2 antibody
    • ATGR2 antibody
    • MRX 88 antibody
    • MRX88 antibody
    • Type 2 angiotensin II receptor antibody
    • Type-2 angiotensin II receptor antibody
    see all

Images

  • ab19134 at 1/2000 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and blocked with serum prior to incubation with the antibody for 18 hours. An HRP conjugated horse polyclonal antibody was used as the secondary.

    See Abreview

  • ICC/IF image of ab19134 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19134, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Bhat SA  et al. AT2R Activation Prevents Microglia Pro-inflammatory Activation in a NOX-Dependent Manner: Inhibition of PKC Activation and p47phox Phosphorylation by PP2A. Mol Neurobiol 56:3005-3023 (2019). Read more (PubMed: 30076526) »
  • Patel SN  et al. Angiotensin II Type 2 Receptor and Receptor Mas Are Colocalized and Functionally Interdependent in Obese Zucker Rat Kidney. Hypertension 70:831-838 (2017). Read more (PubMed: 28827476) »
See all 37 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

1-10 of 10 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Bos taurus Tissue sections (ovary follicle)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate and citric acid buffer
Permeabilization
No
Specification
ovary follicle
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Nov 17 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated
Sample
Mouse Tissue sections (Aorta)
Specification
Aorta
Permeabilization
No
Fixative
Formalin 10%

Abcam user community

Verified customer

Submitted Jul 21 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (epididymis)
Specification
epididymis
Fixative
bouin's solution
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 12 2013

Application
Western blot
Sample
Human Tissue lysate - whole (Brain)
Loading amount
30 µg
Specification
Brain
Treatment
Diluted 1:2 in sample buffer treated with ß-mercaptoethanol
Gel Running Conditions
Reduced Non-Denaturing (Native) (4-20% Tris-HCl pre-cast gels)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

Miss. Laura Palmer

Verified customer

Submitted Nov 19 2012

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Placenta)
Specification
Placenta
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1.5% · Temperature: 20°C

Dr. Paula Williams

Verified customer

Submitted Jul 23 2010

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (RAT PENIS)
Specification
RAT PENIS
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.4% TRITON/PBS
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 15µg/mL · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 17 2008

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain)
Specification
Brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 1%

Miss. Laura Palmer

Verified customer

Submitted May 23 2007

Application
Western blot
Sample
Dog Tissue lysate - whole (heart)
Loading amount
50 µg
Specification
heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Dr. sudhish mishra

Verified customer

Submitted Apr 23 2007

Application
Western blot
Sample
Rat Cell lysate - whole cell (aorta)
Loading amount
40 µg
Specification
aorta
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Dr. Qinhong Zhang

Verified customer

Submitted Jan 10 2007

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human Penis)
Specification
Human Penis
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Dako Peroxidase Blocking Reagent as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100

Miss. Gwen Lagoda

Verified customer

Submitted Jan 09 2007

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up