Recombinant
RabMAb

Recombinant Anti-Angiotensin II Type 2 Receptor antibody [EPR3876] (ab92445)

Rabbit recombinant monoclonal Angiotensin II Type 2 Receptor antibody [EPR3876]. Validated in WB, IP and tested in Mouse, Rat, Human. Cited in 14 publication(s). Independently reviewed in 1 review(s).

Overview

  • Product name

    Anti-Angiotensin II Type 2 Receptor antibody [EPR3876]
    See all Angiotensin II Type 2 Receptor primary antibodies
  • Description

    Rabbit monoclonal [EPR3876] to Angiotensin II Type 2 Receptor
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Angiotensin II Type 2 Receptor aa 300-400 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HepG2, Human heart, Mouse heart, and Rat heart lysates. IP: Mouse heart cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab92445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    Images

    • All lanes : Anti-Angiotensin II Type 2 Receptor antibody [EPR3876] (ab92445) at 1/1000 dilution (Purified)

      Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
      Lane 2 : Human heart lysates
      Lane 3 : Mouse heart lysates
      Lane 4 : Rat heart lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 41 kDa
      Observed band size: 41 kDa

    • ab92445 (purified ) at 1/20 dilution (0.2ug) immunoprecipitating Angiotensin II Type 2 Receptor in Mouse heart lysate .
      Lane 1 (input): Mouse heart lysate 10ug
      Lane 2 (+): ab92445 & Mouse heart lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab92445 in Mouse heart lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    References

    This product has been referenced in:

    See all 17 Publications for this product

    Customer reviews and Q&As

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    1-2 of 2 Abreviews

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (heart)
    Gel Running Conditions
    Non-reduced Denaturing (12%)
    Loading amount
    30 µg
    Specification
    heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Mar 01 2019

    Application
    Western blot
    Sample
    Pig Tissue lysate - whole (Newborn kidney)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    40 µg
    Specification
    Newborn kidney
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Dec 28 2017

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