Product nameAnti-Angiotensin II Type 2 Receptor antibody [EPR3876]
See all Angiotensin II Type 2 Receptor primary antibodies
DescriptionRabbit monoclonal [EPR3876] to Angiotensin II Type 2 Receptor
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Angiotensin II Type 2 Receptor aa 300-400 (C terminal). The exact sequence is proprietary.
- WB: HepG2, Human heart, Mouse heart, and Rat heart lysates. IP: Mouse heart cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab92445 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 41 kDa.|
|IP||1/10 - 1/100.|
FunctionReceptor for angiotensin II. Cooperates with MTUS1 to inhibit ERK2 activation and cell proliferation.
Tissue specificityIn adult, highly expressed in myometrium with lower levels in adrenal gland and fallopian tube. Very highly expressed in fetal kidney and intestine.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- AGTR 2 antibody
- Agtr2 antibody
- AGTR2_HUMAN antibody
All lanes : Anti-Angiotensin II Type 2 Receptor antibody [EPR3876] (ab92445) at 1/1000 dilution (Purified)
Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lane 2 : Human heart lysates
Lane 3 : Mouse heart lysates
Lane 4 : Rat heart lysates
Lysates/proteins at 15 µg per lane.
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
ab92445 (purified ) at 1/20 dilution (0.2ug) immunoprecipitating Angiotensin II Type 2 Receptor in Mouse heart lysate .
Lane 1 (input): Mouse heart lysate 10ug
Lane 2 (+): ab92445 & Mouse heart lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab92445 in Mouse heart lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
This product has been referenced in:
- Shi W et al. Puerarin Reduces Blood Pressure in Spontaneously Hypertensive Rats by Targeting eNOS. Am J Chin Med 47:19-38 (2019). Read more (PubMed: 30612457) »
- Papali'i-Curtin JC et al. Expression of Components of the Renin-Angiotensin System in Pyogenic Granuloma. Front Surg 6:13 (2019). Read more (PubMed: 31024924) »