Recombinant
RabMAb

Recombinant Anti-Angiotensinogen antibody [EPR20599] (ab213705)

Overview

  • Product name

    Anti-Angiotensinogen antibody [EPR20599]
    See all Angiotensinogen primary antibodies
  • Description

    Rabbit monoclonal [EPR20599] to Angiotensinogen
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Mouse Angiotensinogen aa 150-250. The exact sequence is proprietary.
    Database link: P11859

  • Positive control

    • WB: Hepa1-6, mIMCD3, Neuro-2a, HepG2, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Mouse and rat liver, brain and heart lysates; Human fetal heart and fetal kidney lysates. IP: Mouse liver lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab213705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
IP 1/40.

Target

  • Function

    Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. In response to lowered blood pressure, the enzyme renin cleaves angiotensinogen to produce angiotensin-1 (angiotensin 1-10). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2 (angiotensin 1-8). Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3 (angiotensin 2-8), angiotensin-4 (angiotensin 3-8). Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin). Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2.
    Angiotensin-2 acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone.
    Angiotensin-3 stimulates aldosterone release.
    Angiotensin 1-7 is a ligand for the G-protein coupled receptor MAS1 (By similarity). Has vasodilator and antidiuretic effects (By similarity). Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets.
  • Tissue specificity

    Expressed by the liver and secreted in plasma.
  • Involvement in disease

    Genetic variations in AGT are a cause of susceptibility to essential hypertension (EHT) [MIM:145500]. Essential hypertension is a condition in which blood pressure is consistently higher than normal with no identifiable cause.
    Defects in AGT are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
  • Sequence similarities

    Belongs to the serpin family.
  • Post-translational
    modifications

    Beta-decarboxylation of Asp-34 in angiotensin-2, by mononuclear leukocytes produces alanine. The resulting peptide form, angiotensin-A, has the same affinity for the AT1 receptor as angiotensin-2, but a higher affinity for the AT2 receptor.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Aangiotensinogen (serpin peptidase inhibitor clade A member 8) antibody
    • AGT antibody
    • AI265500 antibody
    • Alpha 1 antiproteinase antitrypsin antibody
    • Ang antibody
    • Ang I antibody
    • Ang II antibody
    • Ang III antibody
    • AngII antibody
    • Angiotensin I antibody
    • Angiotensin II antibody
    • Angiotensin III antibody
    • Angiotensin-3 antibody
    • Angiotensinogen (PAT) antibody
    • Angiotensinogen antibody
    • ANGT_HUMAN antibody
    • ANHU antibody
    • ANRT antibody
    • AT-2 antibody
    • AT-II antibody
    • Des-Asp[1]-angiotensin II antibody
    • FLJ92595 antibody
    • FLJ97926 antibody
    • MGC105326 antibody
    • PAT antibody
    • Pre angiotensinogen antibody
    • Serine (or cysteine) proteinase inhibitor antibody
    • Serpin A8 antibody
    • Serpin peptidase inhibitor clade A member 8 antibody
    • SERPINA8 antibody
    see all

Images

  • All lanes : Anti-Angiotensinogen antibody [EPR20599] (ab213705) at 1/1000 dilution

    Lane 1 : Hepa1-6 (Mouse hepatoma epithelial cell line) whole cell lysate
    Lane 2 : mIMCD3 (Mouse inner medullary collecting duct epithelial cell line) whole cell lysate
    Lane 3 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate
    Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 5 : Mouse liver lysate
    Lane 6 : Rat liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 51 kDa
    Observed band size: 51 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1-4: 1 minute; Lane 5: 5 seconds; Lane 6: 3 seconds.

    The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 22518004).

  • All lanes : Anti-Angiotensinogen antibody [EPR20599] (ab213705) at 1/1000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Rat heart lysate
    Lane 3 : Mouse brain lysate
    Lane 4 : Mouse heart lysate
    Lane 5 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 7 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 51 kDa
    Observed band size: 51 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1-4: 1 second; Lane 5-7: 15 seconds.

    The expression profile observed is consistent with what has been described in the literature for mouse heart (PMID: 25085967) or brain (PMID:21628469).

  • All lanes : Anti-Angiotensinogen antibody [EPR20599] (ab213705) at 1/1000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

    Predicted band size: 51 kDa
    Observed band size: 51 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Angiotensinogen was immunoprecipitated from 0.35 mg of mouse liver lysate with ab213705 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab213705 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

    Lane 1: Mouse liver lysate 10 µg (Input).

    Lane 2: ab213705 IP in mouse liver lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213705 in mouse liver lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

References

ab213705 has not yet been referenced specifically in any publications.

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