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    angiotensinogen-antibody-epr20599-ab213705.pdf

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RecombinantRabMAb

Recombinant Anti-Angiotensinogen antibody [EPR20599] (ab213705)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Western blot - Anti-Angiotensinogen antibody [EPR20599] (ab213705)
  • Western blot - Anti-Angiotensinogen antibody [EPR20599] (ab213705)
  • Western blot - Anti-Angiotensinogen antibody [EPR20599] (ab213705)
  • Immunoprecipitation - Anti-Angiotensinogen antibody [EPR20599] (ab213705)
  • Anti-Angiotensinogen antibody [EPR20599] (ab213705)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20599] to Angiotensinogen
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Protein
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Recombinant Human Angiotensinogen protein (BSA and azide free) (ab178470)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
ELISA
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Rat BDNF ELISA Kit (ab213899)

View more associated products

Overview

  • Product name

    Anti-Angiotensinogen antibody [EPR20599]
    See all Angiotensinogen primary antibodies
  • Description

    Rabbit monoclonal [EPR20599] to Angiotensinogen
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Hepa1-6, mIMCD3, Neuro-2a, HepG2, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Mouse and rat liver, brain and heart lysates; Human fetal heart and fetal kidney lysates. IP: Mouse liver lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20599
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Fibrinolysis / Thrombolysis
    • Cardiovascular
    • Blood
    • Other
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Cardiovascular
    • Atherosclerosis
    • Lipoprotein metabolism
    • Cardiovascular
    • Atherosclerosis
    • Apoptosis
    • Cardiovascular
    • Atherosclerosis
    • Hypertension
    • Vasoconstriction
    • Other
    • Cardiovascular
    • Blood
    • Blood Pressure regulation
    • Cardiovascular
    • Heart
    • Cardiac arrhythmias
    • Cardiovascular
    • Heart
    • Apoptosis
    • Cardiovascular
    • Heart
    • Contractility
    • Inotropics
    • Cardiovascular
    • Vasculature
    • Vasoconstriction
    • Other
    • Cardiovascular
    • Vasculature
    • Vasodilation
    • Nitric oxide associated
    • Cardiovascular
    • Vasculature
    • Vasodilation
    • Other
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipoprotein metabolism
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Alternative Versions

    • Anti-Angiotensinogen antibody [EPR20599] - BSA and Azide free (ab240389)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Angiotensinogen protein (BSA and azide free) (ab178470)
  • Related Products

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab213705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
IP
1/40.
Notes
WB
1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
IP
1/40.

Target

  • Function

    Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. In response to lowered blood pressure, the enzyme renin cleaves angiotensinogen to produce angiotensin-1 (angiotensin 1-10). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2 (angiotensin 1-8). Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3 (angiotensin 2-8), angiotensin-4 (angiotensin 3-8). Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin). Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2.
    Angiotensin-2 acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone.
    Angiotensin-3 stimulates aldosterone release.
    Angiotensin 1-7 is a ligand for the G-protein coupled receptor MAS1 (By similarity). Has vasodilator and antidiuretic effects (By similarity). Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets.
  • Tissue specificity

    Expressed by the liver and secreted in plasma.
  • Involvement in disease

    Genetic variations in AGT are a cause of susceptibility to essential hypertension (EHT) [MIM:145500]. Essential hypertension is a condition in which blood pressure is consistently higher than normal with no identifiable cause.
    Defects in AGT are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
  • Sequence similarities

    Belongs to the serpin family.
  • Post-translational
    modifications

    Beta-decarboxylation of Asp-34 in angiotensin-2, by mononuclear leukocytes produces alanine. The resulting peptide form, angiotensin-A, has the same affinity for the AT1 receptor as angiotensin-2, but a higher affinity for the AT2 receptor.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P01019 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 183 Human
    • Entrez Gene: 11606 Mouse
    • Entrez Gene: 24179 Rat
    • Omim: 106150 Human
    • SwissProt: P01019 Human
    • SwissProt: P11859 Mouse
    • SwissProt: P01015 Rat
    • Unigene: 19383 Human
    • Unigene: 301626 Mouse
    • Unigene: 6319 Rat
    see all
  • Alternative names

    • Aangiotensinogen (serpin peptidase inhibitor clade A member 8) antibody
    • AGT antibody
    • AI265500 antibody
    • Alpha 1 antiproteinase antitrypsin antibody
    • Ang antibody
    • Ang I antibody
    • Ang II antibody
    • Ang III antibody
    • AngII antibody
    • Angiotensin I antibody
    • Angiotensin II antibody
    • Angiotensin III antibody
    • Angiotensin-3 antibody
    • Angiotensinogen (PAT) antibody
    • Angiotensinogen antibody
    • ANGT_HUMAN antibody
    • ANHU antibody
    • ANRT antibody
    • AT-2 antibody
    • AT-II antibody
    • Des-Asp[1]-angiotensin II antibody
    • FLJ92595 antibody
    • FLJ97926 antibody
    • MGC105326 antibody
    • PAT antibody
    • Pre angiotensinogen antibody
    • Serine (or cysteine) proteinase inhibitor antibody
    • Serpin A8 antibody
    • Serpin peptidase inhibitor clade A member 8 antibody
    • SERPINA8 antibody
    see all

Images

  • Western blot - Anti-Angiotensinogen antibody [EPR20599] (ab213705)
    Western blot - Anti-Angiotensinogen antibody [EPR20599] (ab213705)
    All lanes : Anti-Angiotensinogen antibody [EPR20599] (ab213705) at 1/1000 dilution

    Lane 1 : Hepa1-6 (Mouse hepatoma epithelial cell line) whole cell lysate
    Lane 2 : mIMCD3 (Mouse inner medullary collecting duct epithelial cell line) whole cell lysate
    Lane 3 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate
    Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 5 : Mouse liver lysate
    Lane 6 : Rat liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 51 kDa
    Observed band size: 51 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1-4: 1 minute; Lane 5: 5 seconds; Lane 6: 3 seconds.

    The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 22518004).

  • Western blot - Anti-Angiotensinogen antibody [EPR20599] (ab213705)
    Western blot - Anti-Angiotensinogen antibody [EPR20599] (ab213705)
    All lanes : Anti-Angiotensinogen antibody [EPR20599] (ab213705) at 1/1000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Rat heart lysate
    Lane 3 : Mouse brain lysate
    Lane 4 : Mouse heart lysate
    Lane 5 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 7 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 51 kDa
    Observed band size: 51 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1-4: 1 second; Lane 5-7: 15 seconds.

    The expression profile observed is consistent with what has been described in the literature for mouse heart (PMID: 25085967) or brain (PMID:21628469).

  • Western blot - Anti-Angiotensinogen antibody [EPR20599] (ab213705)
    Western blot - Anti-Angiotensinogen antibody [EPR20599] (ab213705)
    All lanes : Anti-Angiotensinogen antibody [EPR20599] (ab213705) at 1/1000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

    Predicted band size: 51 kDa
    Observed band size: 51 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-Angiotensinogen antibody [EPR20599] (ab213705)
    Immunoprecipitation - Anti-Angiotensinogen antibody [EPR20599] (ab213705)

    Angiotensinogen was immunoprecipitated from 0.35 mg of mouse liver lysate with ab213705 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab213705 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

    Lane 1: Mouse liver lysate 10 µg (Input).

    Lane 2: ab213705 IP in mouse liver lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213705 in mouse liver lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Anti-Angiotensinogen antibody [EPR20599] (ab213705)
    Anti-Angiotensinogen antibody [EPR20599] (ab213705)

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (2)

Publishing research using ab213705? Please let us know so that we can cite the reference in this datasheet.

ab213705 has been referenced in 2 publications.

  • Zhou G  et al. The regulation effect of WNT-RAS signaling in hypothalamic paraventricular nucleus on renal fibrosis. J Nephrol 33:289-297 (2020). PubMed: 31392659
  • Klein JD & Wang XH Electrically stimulated acupuncture increases renal blood flow through exosome-carried miR-181. Am J Physiol Renal Physiol 315:F1542-F1549 (2018). PubMed: 30132347

Customer reviews and Q&As

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