Recombinant
RabMAb

Recombinant Anti-Angiotensinogen antibody [EPR22199-203] (ab229005)

Overview

  • Product name

    Anti-Angiotensinogen antibody [EPR22199-203]
    See all Angiotensinogen primary antibodies
  • Description

    Rabbit monoclonal [EPR22199-203] to Angiotensinogen
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment within Mouse Angiotensinogen aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P11859

  • Positive control

    • WB: Mouse liver, kidney, heart, brain and lung tissue lysate. IHC-P: Mouse kidney and testis tissue. IP: Mouse liver tissue lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab229005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 53 kDa.
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/30.

Target

  • Function

    Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. In response to lowered blood pressure, the enzyme renin cleaves angiotensinogen to produce angiotensin-1 (angiotensin 1-10). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2 (angiotensin 1-8). Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3 (angiotensin 2-8), angiotensin-4 (angiotensin 3-8). Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin). Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2.
    Angiotensin-2 acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone.
    Angiotensin-3 stimulates aldosterone release.
    Angiotensin 1-7 is a ligand for the G-protein coupled receptor MAS1 (By similarity). Has vasodilator and antidiuretic effects (By similarity). Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets.
  • Tissue specificity

    Expressed by the liver and secreted in plasma.
  • Involvement in disease

    Genetic variations in AGT are a cause of susceptibility to essential hypertension (EHT) [MIM:145500]. Essential hypertension is a condition in which blood pressure is consistently higher than normal with no identifiable cause.
    Defects in AGT are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
  • Sequence similarities

    Belongs to the serpin family.
  • Post-translational
    modifications

    Beta-decarboxylation of Asp-34 in angiotensin-2, by mononuclear leukocytes produces alanine. The resulting peptide form, angiotensin-A, has the same affinity for the AT1 receptor as angiotensin-2, but a higher affinity for the AT2 receptor.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Aangiotensinogen (serpin peptidase inhibitor clade A member 8) antibody
    • AGT antibody
    • AI265500 antibody
    • Alpha 1 antiproteinase antitrypsin antibody
    • Ang antibody
    • Ang I antibody
    • Ang II antibody
    • Ang III antibody
    • AngII antibody
    • Angiotensin I antibody
    • Angiotensin II antibody
    • Angiotensin III antibody
    • Angiotensin-3 antibody
    • Angiotensinogen (PAT) antibody
    • Angiotensinogen antibody
    • ANGT_HUMAN antibody
    • ANHU antibody
    • ANRT antibody
    • AT-2 antibody
    • AT-II antibody
    • Des-Asp[1]-angiotensin II antibody
    • FLJ92595 antibody
    • FLJ97926 antibody
    • MGC105326 antibody
    • PAT antibody
    • Pre angiotensinogen antibody
    • Serine (or cysteine) proteinase inhibitor antibody
    • Serpin A8 antibody
    • Serpin peptidase inhibitor clade A member 8 antibody
    • SERPINA8 antibody
    see all

Images

  • All lanes : Anti-Angiotensinogen antibody [EPR22199-203] (ab229005) at 1/1000 dilution

    Lane 1 : Mouse liver tissue lysate
    Lane 2 : Mouse kidney tissue lysate
    Lane 3 : Mouse heart tissue lysate
    Lane 4 : Mouse brain tissue lysate
    Lane 5 : Mouse lung tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 53 kDa


    Exposure time: 114 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

     

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue stained for Angiotensinogen with ab229005 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Cytoplasmic staining in the proximal tubules of mouse kidney (PMID: 7648615) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG ready to use.

    Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

     

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue stained for Angiotensinogen with ab229005 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Cytoplasmic staining in the Leydig cells of mouse testis (PMID: 20361351) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG ready to use.

    Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

     

  • Angiotensinogen was immunoprecipitated from 0.35 mg mouse liver tissue lysates with ab229005 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229005 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

    Lane 1: Mouse liver lysate (10 μg) (Input).
    Lane 2: ab29005 IP in mouse liver lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229005 in mouse liver lysate (-).

    Blocking/D ilution buffer: 5% NFDM/TBST.
    Exposure time: 10 seconds.

References

ab229005 has not yet been referenced specifically in any publications.

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