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    angiotensinogen-antibody-epr29302-ab108294.pdf

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RecombinantRabMAb

Recombinant Anti-Angiotensinogen antibody [EPR2930(2)] (ab108294)

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Western blot - Anti-Angiotensinogen antibody [EPR2930(2)] (ab108294)
  • Anti-Angiotensinogen antibody [EPR2930(2)] (ab108294)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2930(2)] to Angiotensinogen
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-Angiotensinogen antibody [EPR2930(2)]
    See all Angiotensinogen primary antibodies
  • Description

    Rabbit monoclonal [EPR2930(2)] to Angiotensinogen
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Fetal kidney, fetal artery, fetal heart, HepG2, and Human plasma lysates
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2930(2)
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Fibrinolysis / Thrombolysis
    • Cardiovascular
    • Blood
    • Other
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Cardiovascular
    • Atherosclerosis
    • Lipoprotein metabolism
    • Cardiovascular
    • Atherosclerosis
    • Apoptosis
    • Cardiovascular
    • Atherosclerosis
    • Hypertension
    • Vasoconstriction
    • Other
    • Cardiovascular
    • Blood
    • Blood Pressure regulation
    • Cardiovascular
    • Heart
    • Cardiac arrhythmias
    • Cardiovascular
    • Heart
    • Apoptosis
    • Cardiovascular
    • Heart
    • Contractility
    • Inotropics
    • Cardiovascular
    • Vasculature
    • Vasoconstriction
    • Other
    • Cardiovascular
    • Vasculature
    • Vasodilation
    • Nitric oxide associated
    • Cardiovascular
    • Vasculature
    • Vasodilation
    • Other
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipoprotein metabolism
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Alternative Versions

    • Anti-Angiotensinogen antibody [EPR2930(2)] - BSA and Azide free (ab247620)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Angiotensinogen protein (BSA and azide free) (ab178470)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 53 kDa.
Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 53 kDa.
Application notes
Is unsuitable for IHC-P.

Target

  • Function

    Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. In response to lowered blood pressure, the enzyme renin cleaves angiotensinogen to produce angiotensin-1 (angiotensin 1-10). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2 (angiotensin 1-8). Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3 (angiotensin 2-8), angiotensin-4 (angiotensin 3-8). Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin). Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2.
    Angiotensin-2 acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone.
    Angiotensin-3 stimulates aldosterone release.
    Angiotensin 1-7 is a ligand for the G-protein coupled receptor MAS1 (By similarity). Has vasodilator and antidiuretic effects (By similarity). Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets.
  • Tissue specificity

    Expressed by the liver and secreted in plasma.
  • Involvement in disease

    Genetic variations in AGT are a cause of susceptibility to essential hypertension (EHT) [MIM:145500]. Essential hypertension is a condition in which blood pressure is consistently higher than normal with no identifiable cause.
    Defects in AGT are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
  • Sequence similarities

    Belongs to the serpin family.
  • Post-translational
    modifications

    Beta-decarboxylation of Asp-34 in angiotensin-2, by mononuclear leukocytes produces alanine. The resulting peptide form, angiotensin-A, has the same affinity for the AT1 receptor as angiotensin-2, but a higher affinity for the AT2 receptor.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P01019 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 183 Human
    • Omim: 106150 Human
    • SwissProt: P01019 Human
    • Unigene: 19383 Human
    • Alternative names

      • Aangiotensinogen (serpin peptidase inhibitor clade A member 8) antibody
      • AGT antibody
      • AI265500 antibody
      • Alpha 1 antiproteinase antitrypsin antibody
      • Ang antibody
      • Ang I antibody
      • Ang II antibody
      • Ang III antibody
      • AngII antibody
      • Angiotensin I antibody
      • Angiotensin II antibody
      • Angiotensin III antibody
      • Angiotensin-3 antibody
      • Angiotensinogen (PAT) antibody
      • Angiotensinogen antibody
      • ANGT_HUMAN antibody
      • ANHU antibody
      • ANRT antibody
      • AT-2 antibody
      • AT-II antibody
      • Des-Asp[1]-angiotensin II antibody
      • FLJ92595 antibody
      • FLJ97926 antibody
      • MGC105326 antibody
      • PAT antibody
      • Pre angiotensinogen antibody
      • Serine (or cysteine) proteinase inhibitor antibody
      • Serpin A8 antibody
      • Serpin peptidase inhibitor clade A member 8 antibody
      • SERPINA8 antibody
      see all

    Images

    • Western blot - Anti-Angiotensinogen antibody [EPR2930(2)] (ab108294)
      Western blot - Anti-Angiotensinogen antibody [EPR2930(2)] (ab108294)
      All lanes : Anti-Angiotensinogen antibody [EPR2930(2)] (ab108294) at 1/10000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : Human fetal artery lysate
      Lane 3 : Human fetal heart lysate
      Lane 4 : HepG2 cell lysate
      Lane 5 : Human plasma lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 53 kDa

    • Anti-Angiotensinogen antibody [EPR2930(2)] (ab108294)
      Anti-Angiotensinogen antibody [EPR2930(2)] (ab108294)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab108294? Please let us know so that we can cite the reference in this datasheet.

    ab108294 has been referenced in 2 publications.

    • Gangwar A  et al. Intermittent normobaric hypoxia facilitates high altitude acclimatization by curtailing hypoxia-induced inflammation and dyslipidemia. Pflugers Arch N/A:N/A (2019). PubMed: 30980137
    • Pandey VG  et al. Dexamethasone promotes hypertension by allele-specific regulation of the human angiotensinogen gene. J Biol Chem 290:5749-58 (2015). PubMed: 25568318

    Customer reviews and Q&As

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