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    angiotensinogen-antibody-epr2931-ab108334.pdf

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RecombinantRabMAb

Recombinant Anti-Angiotensinogen antibody [EPR2931] (ab108334)

  • Datasheet
  • SDS
Reviews (4)Q&A (2)References (10)

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Western blot - Anti-Angiotensinogen antibody [EPR2931] (ab108334)
  • Western blot - Anti-Angiotensinogen antibody [EPR2931] (ab108334)
  • Western blot - Anti-Angiotensinogen antibody [EPR2931] (ab108334)
  • Immunocytochemistry/ Immunofluorescence - Anti-Angiotensinogen antibody [EPR2931] (ab108334)
  • Anti-Angiotensinogen antibody [EPR2931] (ab108334)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2931] to Angiotensinogen
  • Suitable for: WB, IP, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Recombinant Human Angiotensinogen protein (BSA and azide free) (ab178470)
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Angiotensinogen antibody [EPR2931]
    See all Angiotensinogen primary antibodies
  • Description

    Rabbit monoclonal [EPR2931] to Angiotensinogen
  • Host species

    Rabbit
  • Specificity

    ab108334 is raised against the Human Angiotensinogen protein. This has little homology with mouse or rat Angiotensinogen and in our hands we do not get good results when testing in these species (in western blot the band is weak or absent and at a lower molecular weight than in human samples) therefore we cannot guarantee this product for use in these species. When using this antibody for western blot we recommend blocking with 5% BSA for best results. In our hands, blocking with milk does not give optimal results in WB and some lower molecular weight binding can be observed.
  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal artery, fetal heart, fetal liver, adult kidney, HepG2, 293T, and plasma lysates; HepG2 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2931
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Fibrinolysis / Thrombolysis
    • Cardiovascular
    • Blood
    • Other
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Cardiovascular
    • Atherosclerosis
    • Lipoprotein metabolism
    • Cardiovascular
    • Atherosclerosis
    • Apoptosis
    • Cardiovascular
    • Atherosclerosis
    • Hypertension
    • Vasoconstriction
    • Other
    • Cardiovascular
    • Blood
    • Blood Pressure regulation
    • Cardiovascular
    • Heart
    • Cardiac arrhythmias
    • Cardiovascular
    • Heart
    • Apoptosis
    • Cardiovascular
    • Heart
    • Contractility
    • Inotropics
    • Cardiovascular
    • Vasculature
    • Vasoconstriction
    • Other
    • Cardiovascular
    • Vasculature
    • Vasodilation
    • Nitric oxide associated
    • Cardiovascular
    • Vasculature
    • Vasodilation
    • Other
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipoprotein metabolism
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease

Associated products

  • Alternative Versions

    • Anti-Angiotensinogen antibody [EPR2931] - BSA and Azide free (ab215869)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Angiotensinogen protein (BSA and azide free) (ab178470)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 53 kDa.

Block with 5% BSA

IP
1/10 - 1/100.
ICC/IF (1)
1/100 - 1/250.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 53 kDa.

Block with 5% BSA

IP
1/10 - 1/100.
ICC/IF
1/100 - 1/250.
Application notes
Is unsuitable for Flow Cyt or IHC-P.

Target

  • Function

    Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. In response to lowered blood pressure, the enzyme renin cleaves angiotensinogen to produce angiotensin-1 (angiotensin 1-10). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2 (angiotensin 1-8). Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3 (angiotensin 2-8), angiotensin-4 (angiotensin 3-8). Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin). Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2.
    Angiotensin-2 acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone.
    Angiotensin-3 stimulates aldosterone release.
    Angiotensin 1-7 is a ligand for the G-protein coupled receptor MAS1 (By similarity). Has vasodilator and antidiuretic effects (By similarity). Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets.
  • Tissue specificity

    Expressed by the liver and secreted in plasma.
  • Involvement in disease

    Genetic variations in AGT are a cause of susceptibility to essential hypertension (EHT) [MIM:145500]. Essential hypertension is a condition in which blood pressure is consistently higher than normal with no identifiable cause.
    Defects in AGT are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
  • Sequence similarities

    Belongs to the serpin family.
  • Post-translational
    modifications

    Beta-decarboxylation of Asp-34 in angiotensin-2, by mononuclear leukocytes produces alanine. The resulting peptide form, angiotensin-A, has the same affinity for the AT1 receptor as angiotensin-2, but a higher affinity for the AT2 receptor.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P01019 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 183 Human
    • Entrez Gene: 11606 Mouse
    • Entrez Gene: 24179 Rat
    • Omim: 106150 Human
    • SwissProt: P01019 Human
    • SwissProt: P11859 Mouse
    • SwissProt: P01015 Rat
    • Unigene: 19383 Human
    • Unigene: 301626 Mouse
    • Unigene: 6319 Rat
    see all
  • Alternative names

    • Aangiotensinogen (serpin peptidase inhibitor clade A member 8) antibody
    • AGT antibody
    • AI265500 antibody
    • Alpha 1 antiproteinase antitrypsin antibody
    • Ang antibody
    • Ang I antibody
    • Ang II antibody
    • Ang III antibody
    • AngII antibody
    • Angiotensin I antibody
    • Angiotensin II antibody
    • Angiotensin III antibody
    • Angiotensin-3 antibody
    • Angiotensinogen (PAT) antibody
    • Angiotensinogen antibody
    • ANGT_HUMAN antibody
    • ANHU antibody
    • ANRT antibody
    • AT-2 antibody
    • AT-II antibody
    • Des-Asp[1]-angiotensin II antibody
    • FLJ92595 antibody
    • FLJ97926 antibody
    • MGC105326 antibody
    • PAT antibody
    • Pre angiotensinogen antibody
    • Serine (or cysteine) proteinase inhibitor antibody
    • Serpin A8 antibody
    • Serpin peptidase inhibitor clade A member 8 antibody
    • SERPINA8 antibody
    see all

Images

  • Western blot - Anti-Angiotensinogen antibody [EPR2931] (ab108334)
    Western blot - Anti-Angiotensinogen antibody [EPR2931] (ab108334)
    All lanes : Anti-Angiotensinogen antibody [EPR2931] (ab108334) at 1/1000 dilution

    Lane 1 : Mouse kidney whole cell lysate
    Lane 2 : Rat kidney whole cell lysate
    Lane 3 : Human kidney whole cell lysate
    Lane 4 : Mouse liver whole cell lysate
    Lane 5 : Rat liver whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG (H+L) peroxidase conjugated. at 1/2000 dilution

    Predicted band size: 53 kDa


    Exposure time: 1 minute


    Ab108334 targeting angiotensinogen in various mouse, rat and human tissues (see lane descriptions).

    Blocking buffer for the first lanes 1-5: 5% NFDM/TBST; the second lanes 1-5: BSA/TBST.

  • Western blot - Anti-Angiotensinogen antibody [EPR2931] (ab108334)
    Western blot - Anti-Angiotensinogen antibody [EPR2931] (ab108334)
    All lanes : Anti-Angiotensinogen antibody [EPR2931] (ab108334) at 1/1000 dilution

    All lanes : Human fetal liver whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG (H+L) peroxidase conjugated. at 1/2000 dilution

    Predicted band size: 53 kDa


    Exposure time: 3 minutes


    Lane 1 blocking buffer: NFDM/TBST

    Lane 2 blocking buffer: BSA/TBST

  • Western blot - Anti-Angiotensinogen antibody [EPR2931] (ab108334)
    Western blot - Anti-Angiotensinogen antibody [EPR2931] (ab108334)
    All lanes : Anti-Angiotensinogen antibody [EPR2931] (ab108334) at 1/1000 dilution

    Lane 1 : Human fetal artery lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal liver lysate
    Lane 4 : HepG2 lysate
    Lane 5 : 293T lysate
    Lane 6 : Human plasma lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 53 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Angiotensinogen antibody [EPR2931] (ab108334)
    Immunocytochemistry/ Immunofluorescence - Anti-Angiotensinogen antibody [EPR2931] (ab108334)
    ab108334, at 1/100 dilution, staining Angiotensinogen in HepG2 cells by Immunofluorescence.
  • Anti-Angiotensinogen antibody [EPR2931] (ab108334)
    Anti-Angiotensinogen antibody [EPR2931] (ab108334)

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (10)

Publishing research using ab108334? Please let us know so that we can cite the reference in this datasheet.

ab108334 has been referenced in 10 publications.

  • Li S  et al. Bioinformatics analysis identifies biomarkers associated with poor prognosis in diffuse-type gastric cancer. Mol Med Rep 23:N/A (2021). PubMed: 33495829
  • Zhang T  et al. AT1R knockdown confers cardioprotection against sepsis-induced myocardial injury by inhibiting the MAPK signaling pathway in rats. J Cell Biochem 121:25-42 (2020). PubMed: 31433522
  • Sharma NM  et al. A novel role for miR-133a in centrally mediated activation of the renin-angiotensin system in congestive heart failure. Am J Physiol Heart Circ Physiol 312:H968-H979 (2017). PubMed: 28283551
  • Xiang X  et al. Tuberous sclerosis complex 1-mechanistic target of rapamycin complex 1 signaling determines brown-to-white adipocyte phenotypic switch. Diabetes 64:519-28 (2015). PubMed: 25213336
  • Kemp JR  et al. Angiotensin II-regulated microRNA 483-3p directly targets multiple components of the renin-angiotensin system. J Mol Cell Cardiol 75:25-39 (2014). PubMed: 24976017
  • Gorman JL  et al. Angiotensin II evokes angiogenic signals within skeletal muscle through co-ordinated effects on skeletal myocytes and endothelial cells. PLoS One 9:e85537 (2014). WB ; Rat, Human . PubMed: 24416421
  • Yan J  et al. Long-term effects of maternal diabetes on blood pressure and renal function in rat male offspring. PLoS One 9:e88269 (2014). WB ; A wide range of other invertebrates . PubMed: 24505458
  • Muñoz MC  et al. Downregulation of the ACE2/Ang-(1-7)/Mas axis in transgenic mice overexpressing GH. J Endocrinol 221:215-27 (2014). Mouse . PubMed: 24756097
  • Paz MC  et al. Involvement of the brain renin-angiotensin system (RAS) in the neuroadaptive responses induced by amphetamine in a two-injection protocol. Behav Brain Res 272:314-23 (2014). PubMed: 25046593
  • Tsukamoto I  et al. Activating types 1 and 2 angiotensin II receptors modulate the hypertrophic differentiation of chondrocytes. FEBS Open Bio 3:279-84 (2013). WB . PubMed: 23905010

Customer reviews and Q&As

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1-4 of 4 Abreviews

Immunocytochemistry abreview for Anti-Angiotensinogen antibody [EPR2931]

Poor
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Mouse Cultured Cells (Cardiomyocytes)
Permeabilization
Yes - Triton x-100, 0.03%
Specification
Cardiomyocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 13 2018

Western blot abreview for Anti-Angiotensinogen antibody [EPR2931]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Cardiomyocytes)
Gel Running Conditions
Reduced Denaturing (10% PAGE)
Loading amount
25 µg
Specification
Cardiomyocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 28 2015

Immunocytochemistry/ Immunofluorescence

Poor
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cultured Cells (human embryonic stem cells)
Specification
human embryonic stem cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Methanol
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 06 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Angiotensinogen antibody [EPR2931]

Good
Abreviews
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (epididymis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
epididymis
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
bouin's solution
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Apr 09 2013

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