Recombinant Anti-Angiotensinogen antibody [EPR2931] (ab108334)
![Western blot - Anti-Angiotensinogen antibody [EPR2931] (ab108334)](https://www.abcam.com/ps/products/108/ab108334/Images/ab108334-256680-anti-angiotensinogen-antibody-epr2931-western-blot.jpg "Western blot - Anti-Angiotensinogen antibody [EPR2931] (ab108334)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2931] to Angiotensinogen
- Suitable for: WB, IP, ICC/IF
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Angiotensinogen antibody [EPR2931]
See all Angiotensinogen primary antibodies -
Description
Rabbit monoclonal [EPR2931] to Angiotensinogen -
Host species
Rabbit -
Specificity
ab108334 is raised against the Human Angiotensinogen protein. This has little homology with mouse or rat Angiotensinogen and in our hands we do not get good results when testing in these species (in western blot the band is weak or absent and at a lower molecular weight than in human samples) therefore we cannot guarantee this product for use in these species. When using this antibody for western blot we recommend blocking with 5% BSA for best results. In our hands, blocking with milk does not give optimal results in WB and some lower molecular weight binding can be observed. -
Tested applications
Suitable for: WB, IP, ICC/IFmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal artery, fetal heart, fetal liver, adult kidney, HepG2, 293T, and plasma lysates; HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2931 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108334 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 53 kDa.
Block with 5% BSA |
| IP |
1/10 - 1/100.
|
|
| ICC/IF | (1) |
1/100 - 1/250.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 53 kDa. Block with 5% BSA |
|
IP
1/10 - 1/100. |
|
ICC/IF
1/100 - 1/250. |
Application notes
Is unsuitable for Flow Cyt or IHC-P.
Target
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Function
Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. In response to lowered blood pressure, the enzyme renin cleaves angiotensinogen to produce angiotensin-1 (angiotensin 1-10). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2 (angiotensin 1-8). Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3 (angiotensin 2-8), angiotensin-4 (angiotensin 3-8). Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin). Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2.
Angiotensin-2 acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone.
Angiotensin-3 stimulates aldosterone release.
Angiotensin 1-7 is a ligand for the G-protein coupled receptor MAS1 (By similarity). Has vasodilator and antidiuretic effects (By similarity). Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Involvement in disease
Genetic variations in AGT are a cause of susceptibility to essential hypertension (EHT) [MIM:145500]. Essential hypertension is a condition in which blood pressure is consistently higher than normal with no identifiable cause.
Defects in AGT are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). -
Sequence similarities
Belongs to the serpin family. -
Post-translational
modificationsBeta-decarboxylation of Asp-34 in angiotensin-2, by mononuclear leukocytes produces alanine. The resulting peptide form, angiotensin-A, has the same affinity for the AT1 receptor as angiotensin-2, but a higher affinity for the AT2 receptor. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 183 Human
- Entrez Gene: 11606 Mouse
- Entrez Gene: 24179 Rat
- Omim: 106150 Human
- SwissProt: P01019 Human
- SwissProt: P11859 Mouse
- SwissProt: P01015 Rat
- Unigene: 19383 Human
see all -
Alternative names
- Aangiotensinogen (serpin peptidase inhibitor clade A member 8) antibody
- AGT antibody
- AI265500 antibody
see all
Images
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Western blot - Anti-Angiotensinogen antibody [EPR2931] (ab108334)All lanes : Anti-Angiotensinogen antibody [EPR2931] (ab108334) at 1/1000 dilution
Lane 1 : Mouse kidney whole cell lysate
Lane 2 : Rat kidney whole cell lysate
Lane 3 : Human kidney whole cell lysate
Lane 4 : Mouse liver whole cell lysate
Lane 5 : Rat liver whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG (H+L) peroxidase conjugated. at 1/2000 dilution
Predicted band size: 53 kDa
Exposure time: 1 minuteAb108334 targeting angiotensinogen in various mouse, rat and human tissues (see lane descriptions).
Blocking buffer for the first lanes 1-5: 5% NFDM/TBST; the second lanes 1-5: BSA/TBST.
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![Western blot - Anti-Angiotensinogen antibody [EPR2931] (ab108334)](https://www.abcam.com/ps/products/108/ab108334/Images/ab108334-256679-anti-angiotensinogen-antibody-epr2931-western-blot.jpg)
Western blot - Anti-Angiotensinogen antibody [EPR2931] (ab108334)All lanes : Anti-Angiotensinogen antibody [EPR2931] (ab108334) at 1/1000 dilution
All lanes : Human fetal liver whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG (H+L) peroxidase conjugated. at 1/2000 dilution
Predicted band size: 53 kDa
Exposure time: 3 minutesLane 1 blocking buffer: NFDM/TBST
Lane 2 blocking buffer: BSA/TBST
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![Western blot - Anti-Angiotensinogen antibody [EPR2931] (ab108334)](https://www.abcam.com/ps/products/108/ab108334/Images/ab108334-120915-anti-angiotensinogen-antibody-epr2931-western-blot.jpg)
Western blot - Anti-Angiotensinogen antibody [EPR2931] (ab108334)All lanes : Anti-Angiotensinogen antibody [EPR2931] (ab108334) at 1/1000 dilution
Lane 1 : Human fetal artery lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal liver lysate
Lane 4 : HepG2 lysate
Lane 5 : 293T lysate
Lane 6 : Human plasma lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 53 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Angiotensinogen antibody [EPR2931] (ab108334)](https://www.abcam.com/ps/products/108/ab108334/Images/ab108334-120914-anti-angiotensinogen-antibody-epr2931-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Angiotensinogen antibody [EPR2931] (ab108334)ab108334, at 1/100 dilution, staining Angiotensinogen in HepG2 cells by Immunofluorescence. -
![Anti-Angiotensinogen antibody [EPR2931] (ab108334)](https://www.abcam.com/ps/products/108/ab108334/Images/ab108334-6-benefits-of-recombinant-antibodies.png)
Anti-Angiotensinogen antibody [EPR2931] (ab108334)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab108334 has been referenced in 9 publications.
- Zhang T et al. AT1R knockdown confers cardioprotection against sepsis-induced myocardial injury by inhibiting the MAPK signaling pathway in rats. J Cell Biochem 121:25-42 (2020). PubMed: 31433522
- Sharma NM et al. A novel role for miR-133a in centrally mediated activation of the renin-angiotensin system in congestive heart failure. Am J Physiol Heart Circ Physiol 312:H968-H979 (2017). PubMed: 28283551
- Xiang X et al. Tuberous sclerosis complex 1-mechanistic target of rapamycin complex 1 signaling determines brown-to-white adipocyte phenotypic switch. Diabetes 64:519-28 (2015). PubMed: 25213336
- Kemp JR et al. Angiotensin II-regulated microRNA 483-3p directly targets multiple components of the renin-angiotensin system. J Mol Cell Cardiol 75:25-39 (2014). PubMed: 24976017
- Gorman JL et al. Angiotensin II evokes angiogenic signals within skeletal muscle through co-ordinated effects on skeletal myocytes and endothelial cells. PLoS One 9:e85537 (2014). WB ; Rat, Human . PubMed: 24416421
- Yan J et al. Long-term effects of maternal diabetes on blood pressure and renal function in rat male offspring. PLoS One 9:e88269 (2014). WB ; A wide range of other invertebrates . PubMed: 24505458
- Muñoz MC et al. Downregulation of the ACE2/Ang-(1-7)/Mas axis in transgenic mice overexpressing GH. J Endocrinol 221:215-27 (2014). Mouse . PubMed: 24756097
- Paz MC et al. Involvement of the brain renin-angiotensin system (RAS) in the neuroadaptive responses induced by amphetamine in a two-injection protocol. Behav Brain Res 272:314-23 (2014). PubMed: 25046593
- Tsukamoto I et al. Activating types 1 and 2 angiotensin II receptors modulate the hypertrophic differentiation of chondrocytes. FEBS Open Bio 3:279-84 (2013). WB . PubMed: 23905010