Immunocytochemistry/ Immunofluorescence

Poor
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cultured Cells (human embryonic stem cells)
Specification
human embryonic stem cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Methanol

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/200

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-Rabbit Alexa Fluor 568
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 568
Dilution
1/400

Additional data

Additional Notes
I expect human ES cells to be negative for this protein.
Abcam response
Thank you for sharing this data with us. Previously ab108334 has been tested in ICC/IF on HepG2 cells, where it was found to be positive. However, based on this abreview, it seems the antibody may not be suitable in ICC/IF, under these conditions. Abcam would welcome feedback from other customers using this antibody.

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Verified customer

Submitted Jun 06 2013

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