Anti-ANGPTL3 antibody (ab84035)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-ANGPTL3 antibody
See all ANGPTL3 primary antibodies -
DescriptionRabbit polyclonal to ANGPTL3
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Pig
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus ANGPTL3.
Read Abcam's proprietary immunogen policy (Peptide available as ab94376.) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: HepG2; HEK293; A498
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab84035 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Application notes
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 58 & 56 kDa (predicted molecular weight: 54 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Tissue specificityExpressed principally in liver. Weakly expressed in kidney.
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Sequence similaritiesContains 1 fibrinogen C-terminal domain.
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Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 27329 Human
- Omim: 604774 Human
- SwissProt: Q9Y5C1 Human
- Unigene: 209153 Human
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Alternative names
- ANG 5 antibody
- ANG-5 antibody
- ANG5 antibody
see all
Images
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Western blot - Anti-ANGPTL3 antibody (ab84035)All lanes : Anti-ANGPTL3 antibody (ab84035) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
Additional bands at: 56 kDa (possible cleavage fragment)
Exposure time: 20 minutes
ANGPTL3 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. We hypothesize that the 58 and 56 kDa bands represent the precursor and mature proteins, respectively.
Protocols
Datasheets and documents
References
ab84035 has not yet been referenced specifically in any publications.
