Overview

  • Product name
  • Description
    Rabbit polyclonal to ANGPTL3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 71-120 (IFQKLNIFDQ SFYDLSLQTS EIKEEEKELR RTTYKLQVKN EEVKNMSLEL) of Human ANGPTL3 (NP_055310).

  • Positive control
    • COLO205 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab99020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 54 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Anti-ANGPTL3 antibody (ab99020) at 1 µg/ml + COLO205 cell lysate at 10 µg

    Predicted band size: 54 kDa



    Gel concentration: 12%

References

ab99020 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

I have issued a free of charge replacement with the order number **** for the rabbit monoclonal, ab126718.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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Answer

Thank you for having your post-doc provide the protocol and image. Is she confident that the samples are not degraded? The smear around the 49kDa marker suggest that might be the case, but I do not have an explanation for absence of signal for the recombinant protein or the HepG2 samples, unless the recombinant protein is a fragment that does not include the immunogen sequence that the antibody was generated against. She mentioned that a different antibody does detect this recombinant protein. Was that antibody also tested against the serum and HepG2 lysates?

I think the protocol is correct, so I think either the samples are the problem, or the antibody. I expect the post-doc has tested the same samples with other antibodies that give good results. If she is confident that the samples are good, then I suggest we send a replacement, a different antibody against the same protein. I recommend ab126718.

Click here (or use the following: https://www.abcam.com/index.html?datasheet=126718).

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Answer

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.
We are not aware of any problems with this antibody. We have also not received any negative feedback in this regards so far.
Indeed, we have not yet tested this antibody on the mouse material. Even though through sequence homology we predict the antibody to work on mouse material, this would need to be assessed experimentally. Do you have any possibility to test this antibody on human material as a positive control?
I do however appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful so far. I agree that with your protocol and with the experience in your laboratory the results should have been positive. Therefore it either is that the antibody does not crossreact with mouse or has been damaged. Please do let me know whether you have the possibility to test this antibody on human material. Please do also provide me with the order number of the antibody, so I can trace back the vial in our system.
Thank you for your cooperation. Please let me also know if you have any question. I look forward to hearing from you.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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