Overview

  • Product name
  • Description
    Goat polyclonal to Anillin
  • Host species
    Goat
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    WQPDACYKPIGKP

    , corresponding to C terminal amino acids 1113-1125 of Human Anillin.

  • General notes
    GenBank Accession Number – NP_061155

Properties

Applications

Our Abpromise guarantee covers the use of ab5910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
IHC-P Use a concentration of 3 - 10 µg/ml.

Target

  • Function
    Required for cytokinesis. Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression.
  • Tissue specificity
    Ubiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors.
  • Sequence similarities
    Contains 1 PH domain.
  • Developmental stage
    Expressed in fetal brain, heart, kidney, liver, lung, skeletal muscle, spleen and thymus. In dividing cells expression increases during S and G2 phases, peaks at mitosis and subsequently drops as cells enter G1 phase.
  • Post-translational
    modifications
    Phosphorylated during mitosis.
    Ubiquitinated, and this requires FZR1/CDH1.
  • Cellular localization
    Nucleus. Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Mainly found in the nucleus during interphase. Colocalizes with cortical F-actin upon nuclear envelope breakdown in mitosis and subsequently concentrates in the area of the prospective contractile ring in anaphase. This pattern persists until telophase, when the protein becomes concentrated in the midbody.
  • Information by UniProt
  • Database links
  • Alternative names
    • Actin binding protein scraps homolog Drosophila antibody
    • Actin binding protein anillin antibody
    • Actin-binding protein anillin antibody
    • Anillin actin binding protein antibody
    • anln antibody
    • ANLN_HUMAN antibody
    • DKFZp779A055 antibody
    • FSGS8 antibody
    • Scra antibody
    • Scraps antibody
    • Scraps homolog antibody
    see all

Images

  • Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line) and permeabilized with 0.5% Triton using ab5910 . Primary incubation 1hr (10µg/ml) followed by Alexa Fluor 488 secondary antibody (1µg/ml). IgG control: Unimmunized goat IgG (black line) fol
  • ab14404 staining Anillinin Mouse fibroblast cellsby ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with methanol and blocked with 4% BSA for 2 hours at 4°C. Samples were incubated with primary antibody (1/25 in PBS + 1% BSA) for 2 hour at 25°C. An  Alexa Fluor®488-conjugated Donkey anti-goat  polyclonal(1/500) was used as the secondary antibody.

    See Abreview

  • ab5910 at 10ug/ml staining Anillin in human kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in microwave and in Tris/EDTA buffer (pH 9.0). HRP-staining procedure was used for detection.

References

This product has been referenced in:
  • Luján P  et al. PRL-3 disrupts epithelial architecture by altering the post-mitotic midbody position. J Cell Sci 129:4130-4142 (2016). Read more (PubMed: 27656108) »
  • Zhou L  et al. Anxiety and depression with neurogenesis defects in exchange protein directly activated by cAMP 2-deficient mice are ameliorated by a selective serotonin reuptake inhibitor, Prozac. Transl Psychiatry 6:e881 (2016). WB . Read more (PubMed: 27598965) »
See all 6 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Application
Immunocytochemistry
Sample
Mouse Cultured Cells (fibroblasts)
Specification
fibroblasts
Fixative
Paraformaldehyde
Permeabilization
Yes - Methanol
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C

Dr. Manoj B Menon

Verified customer

Submitted Mar 22 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Immortalized Fibroblasts)
Specification
Immortalized Fibroblasts
Fixative
Paraformaldehyde
Permeabilization
Yes - 0,25% Triton-X-100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C

Dr. Manoj B Menon

Verified customer

Submitted Jan 22 2013

Answer

DISCOUNT CODE: xxxxxx
Expiration date: 07/05/13
Value: € 380
I am very pleased to hear you would like to accept our offer and test ab5910 in ICC on mouse samples. Because ICC is an untested application and mouse is also an untested species we recommend it would be beneficial to test human cells in ICC as well. This will help determining if the application or the species is unsuitable in case the experiment does not give satisfactory results.
This code will give you € 380 off your next order before the expiration date. To redeem this offer, please submit an Abreview for ICC and mouse (human) samples and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (hippocampal neurons)
Specification
hippocampal neurons
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10

Abcam user community

Verified customer

Submitted Jun 28 2006

Answer

Dear Professor Hall, Thank you for clarifying that I cannot use the published images without the correct approval! It would be really useful if you would be able to provide some images that we are able to show on our website. Other customers often find it very encouraging to have data from independent sources (i.e other researchers)showing the activity of the antibody. I look forward to hearing from you

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Question
Answer

Thank you for your enquiry. Ab5910 is a "Fast Track antibody:" Fast-Track antibodies are immunogen affinity purified and are shown by ELISA to have high titre values against their immunogen. We are currently testing them for use in Western blotting. Ab5910 has not yet been tested in IHC; a customer has reported some good results in WB and IF (see equiries tab on the online datasheet) but this antibody has not yet been fully characterized. For more information about the Fast Track terms of use, please see the online datasheet. If you have any more questions, please do contact us again.

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Answer

Thank you for your enquiry and your patience. We are very pleased to hear that you have successfully used this product. However, we regret to inform you that we do not ship our products on dry ice especially not within Cambridge area. Did you actually aliquot product and store the vials in the freezer? Did you use a separate aliquot for the next experiment or the same vial? As our datasheet advices - it is very important to avoid repeated freeze / thaw cycles.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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