Anti-Anillin antibody (ab5910)
Key features and details
- Goat polyclonal to Anillin
- Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Anillin antibody
See all Anillin primary antibodies -
Description
Goat polyclonal to Anillin -
Host species
Goat -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow -
Immunogen
Synthetic peptide corresponding to Human Anillin aa 1100 to the C-terminus (C terminal). (NP_001271230.1; NP_001271231.1)
Database link: Q9NQW6 -
Positive control
- ICC/IF: U2OS cells. Flow Cyt (intra): MCF7 cells. IHC-P: Human kidney tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
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|
ICC/IF | (3) |
Use a concentration of 10 µg/ml.
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IHC-P | (1) |
Use a concentration of 3 - 10 µg/ml.
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Notes |
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Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
ICC/IF
Use a concentration of 10 µg/ml. |
IHC-P
Use a concentration of 3 - 10 µg/ml. |
Target
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Function
Required for cytokinesis. Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression. -
Tissue specificity
Ubiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors. -
Sequence similarities
Contains 1 PH domain. -
Developmental stage
Expressed in fetal brain, heart, kidney, liver, lung, skeletal muscle, spleen and thymus. In dividing cells expression increases during S and G2 phases, peaks at mitosis and subsequently drops as cells enter G1 phase. -
Post-translational
modificationsPhosphorylated during mitosis.
Ubiquitinated, and this requires FZR1/CDH1. -
Cellular localization
Nucleus. Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Mainly found in the nucleus during interphase. Colocalizes with cortical F-actin upon nuclear envelope breakdown in mitosis and subsequently concentrates in the area of the prospective contractile ring in anaphase. This pattern persists until telophase, when the protein becomes concentrated in the midbody. - Information by UniProt
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Database links
- Entrez Gene: 54443 Human
- Entrez Gene: 68743 Mouse
- Omim: 616027 Human
- SwissProt: Q9NQW6 Human
- SwissProt: Q8K298 Mouse
- Unigene: 62180 Human
- Unigene: 282751 Mouse
- Unigene: 453238 Mouse
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Alternative names
- Actin binding protein scraps homolog Drosophila antibody
- Actin binding protein anillin antibody
- Actin-binding protein anillin antibody
see all
Images
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ab5910 at 10ug/ml staining Anillin in human kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in microwave and in Tris/EDTA buffer (pH 9.0). HRP-staining procedure was used for detection.
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Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line) and permeabilized with 0.5% Triton using ab5910 . Primary incubation 1hr (10µg/ml) followed by Alexa Fluor 488 secondary antibody (1µg/ml). IgG control: Unimmunized goat IgG (black line) fol
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Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling Anillin with ab5910 at 10 µg/ml. Cells were paraformaldehyde fixed and permeabilized with 0.15% triton. Primary antibody icubation was for 1 hour followed by incubation with an Alexa Fluor® 488-conjugated secondary antibody at 2 µg/ml. DAPI nuclear counterstain was used.
Negative control: Unimmunized goat IgG (10 µg/ml) followed by an Alexa Fluor® 488-conjugated secondary antibody (2 µg/ml).
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ab14404 staining Anillinin Mouse fibroblast cellsby ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with methanol and blocked with 4% BSA for 2 hours at 4°C. Samples were incubated with primary antibody (1/25 in PBS + 1% BSA) for 2 hour at 25°C. An Alexa Fluor®488-conjugated Donkey anti-goat polyclonal(1/500) was used as the secondary antibody.
Protocols
Datasheets and documents
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Datasheet download
References (8)
ab5910 has been referenced in 8 publications.
- Han L et al. Lamin B2 Levels Regulate Polyploidization of Cardiomyocyte Nuclei and Myocardial Regeneration. Dev Cell 53:42-59.e11 (2020). PubMed: 32109383
- Kalebic N et al. Neocortical Expansion Due to Increased Proliferation of Basal Progenitors Is Linked to Changes in Their Morphology. Cell Stem Cell 24:535-550.e9 (2019). PubMed: 30905618
- Luján P et al. PRL-3 disrupts epithelial architecture by altering the post-mitotic midbody position. J Cell Sci 129:4130-4142 (2016). PubMed: 27656108
- Zhou L et al. Anxiety and depression with neurogenesis defects in exchange protein directly activated by cAMP 2-deficient mice are ameliorated by a selective serotonin reuptake inhibitor, Prozac. Transl Psychiatry 6:e881 (2016). WB . PubMed: 27598965
- Stoeckelhuber M et al. Human axillary apocrine glands: proteins involved in the apocrine secretory mechanism. Histol Histopathol 26:177-84 (2011). IHC-P ; Human . PubMed: 21154231
- Stubbs D et al. Neurovascular congruence during cerebral cortical development. Cereb Cortex 19 Suppl 1:i32-41 (2009). PubMed: 19386634
- Bantubungi K et al. Stem cell factor and mesenchymal and neural stem cell transplantation in a rat model of Huntington's disease. Mol Cell Neurosci 37:454-70 (2008). PubMed: 18083596
- Hall PA et al. The septin-binding protein anillin is overexpressed in diverse human tumors. Clin Cancer Res 11:6780-6 (2005). IHC-P ; Human . PubMed: 16203764