Overview

  • Product name

  • Description

    Rabbit polyclonal to Anillin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to a region within residue 1074 - 1124 of Human Anillin (NP_061155.2)

  • Positive control

    • Colon carcinoma; breast carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab84676 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: 1/100 - 1/500. Antigen retrieval with citrate buffer may enhance staining.
    Note: expected to work on frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Required for cytokinesis. Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression.
    • Tissue specificity

      Ubiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors.
    • Sequence similarities

      Contains 1 PH domain.
    • Developmental stage

      Expressed in fetal brain, heart, kidney, liver, lung, skeletal muscle, spleen and thymus. In dividing cells expression increases during S and G2 phases, peaks at mitosis and subsequently drops as cells enter G1 phase.
    • Post-translational
      modifications

      Phosphorylated during mitosis.
      Ubiquitinated, and this requires FZR1/CDH1.
    • Cellular localization

      Nucleus. Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Mainly found in the nucleus during interphase. Colocalizes with cortical F-actin upon nuclear envelope breakdown in mitosis and subsequently concentrates in the area of the prospective contractile ring in anaphase. This pattern persists until telophase, when the protein becomes concentrated in the midbody.
    • Information by UniProt
    • Database links

    • Alternative names

      • Actin binding protein scraps homolog Drosophila antibody
      • Actin binding protein anillin antibody
      • Actin-binding protein anillin antibody
      • Anillin actin binding protein antibody
      • anln antibody
      • ANLN_HUMAN antibody
      • DKFZp779A055 antibody
      • FSGS8 antibody
      • Scra antibody
      • Scraps antibody
      • Scraps homolog antibody
      see all

    Images

    • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human colon carcinoma using 1/250 ab84676, followed by DAB staining.
    • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human breast carcinoma using 1/250 ab84676, followed by DAB staining.

    References

    ab84676 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (colon)
    Specification
    colon
    Fixative
    Acetone
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buufer
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C

    Abcam user community

    Verified customer

    Submitted Dec 22 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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