Anti-Anillin antibody (ab99352)
Key features and details
- Rabbit polyclonal to Anillin
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Anillin antibody
See all Anillin primary antibodies -
Description
Rabbit polyclonal to Anillin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide, corresponding to a region within N terminal amino acids 1-50 of Human Anillin (NP_061155.2)
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab99352 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/2000 - 1/10000. Predicted molecular weight: 124 kDa.
|
|
IP |
Use a concentration of 2 - 5 µg/ml.
|
Notes |
---|
WB
1/2000 - 1/10000. Predicted molecular weight: 124 kDa. |
IP
Use a concentration of 2 - 5 µg/ml. |
Target
-
Function
Required for cytokinesis. Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression. -
Tissue specificity
Ubiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors. -
Sequence similarities
Contains 1 PH domain. -
Developmental stage
Expressed in fetal brain, heart, kidney, liver, lung, skeletal muscle, spleen and thymus. In dividing cells expression increases during S and G2 phases, peaks at mitosis and subsequently drops as cells enter G1 phase. -
Post-translational
modificationsPhosphorylated during mitosis.
Ubiquitinated, and this requires FZR1/CDH1. -
Cellular localization
Nucleus. Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Mainly found in the nucleus during interphase. Colocalizes with cortical F-actin upon nuclear envelope breakdown in mitosis and subsequently concentrates in the area of the prospective contractile ring in anaphase. This pattern persists until telophase, when the protein becomes concentrated in the midbody. - Information by UniProt
-
Database links
- Entrez Gene: 54443 Human
- Omim: 616027 Human
- SwissProt: Q9NQW6 Human
- Unigene: 62180 Human
-
Alternative names
- Actin binding protein scraps homolog Drosophila antibody
- Actin binding protein anillin antibody
- Actin-binding protein anillin antibody
see all
Images
-
All lanes : Anti-Anillin antibody (ab99352) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 124 kDa
Exposure time: 30 seconds -
Detection of Human Anillin by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded). ab99352 used for WB at 1 µg/ml and used for IP at 3 µg/mg lysate (lane 1). Anillin was also immunoprecipitated by another rabbit anti-Anillin antibody (lane 2) and control IgG (lane 3). Detection Chemiluminescence with exposure times of 3 seconds
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (4)
ab99352 has been referenced in 4 publications.
- Cao YF et al. Targeting USP10 induces degradation of oncogenic ANLN in esophageal squamous cell carcinoma. Cell Death Differ 30:527-543 (2023). PubMed: 36526897
- Uretmen Kagiali ZC et al. CLIC4 and CLIC1 bridge plasma membrane and cortical actin network for a successful cytokinesis. Life Sci Alliance 3:N/A (2020). PubMed: 31879279
- S Pedersen R et al. Profiling DNA damage response following mitotic perturbations. Nat Commun 7:13887 (2016). PubMed: 27976684
- Marthandan S et al. Conserved Senescence Associated Genes and Pathways in Primary Human Fibroblasts Detected by RNA-Seq. PLoS One 11:e0154531 (2016). WB . PubMed: 27140416