Key features and details
- Rabbit polyclonal to ANK-3
- Suitable for: IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab213687 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500.
When using a fluorescent probe the recommended dilution is 1/50 – 1/200.
FunctionIn skeletal muscle, required for costamere localization of DMD and betaDAG1 (By similarity). Membrane-cytoskeleton linker. May participate in the maintenance/targeting of ion channels and cell adhesion molecules at the nodes of Ranvier and axonal initial segments. Regulates KCNA1 channel activity in function of dietary Mg(2+) levels, and thereby contributes to the regulation of renal Mg(2+) reabsorption (PubMed:23903368).
Isoform 5: May be part of a Golgi-specific membrane cytoskeleton in association with beta-spectrin.
Tissue specificityExpressed in brain, neurons, muscles and other tissues.
Involvement in diseaseGenetic variations in ANK3 may be associated with autism spectrum disorders susceptibility.
Mental retardation, autosomal recessive 37
Sequence similaritiesContains 23 ANK repeats.
Contains 1 death domain.
Contains 2 ZU5 domains.
Developmental stageUp-regulated during muscle cell differentiation.
DomainThe tandem configuration of the two ZU5 and the UPA domains forms a structural supramodule termed ZZU. ZU5-1 mediates interaction with beta-spectrin, and the ZU5-1/UPA interface is required for ankyrin's function other than binding to spectrin.
Cellular localizationCytoplasm, cytoskeleton. Cell projection, axon. Cell membrane, sarcolemma. Cell junction, synapse, postsynaptic cell membrane. Lysosome. In skeletal muscle, localized at costameres and neuromuscular junctions. In macrophages, associated with lysosomes and Cytoplasm, cytoskeleton. Golgi apparatus.
- Information by UniProt
- ANK-3 antibody
- ANK3 antibody
- ANK3_HUMAN antibody
Immunohistochemical analysis of formalin-fixed and paraffin-embedded Rat brain tissue labeling ANK-3 with ab213687 at 1/200 dilution followed by Goat Anti-Rabbit IgG, Cy3 at 1/200 dilution for 40 minutes at 37°C.
Immunohistochemical analysis of formalin-fixed and paraffin-embedded Rat brain tissue labeling ANK-3 with ab213687 at 1/200 dilution, followed by conjugation to a secondary antibody and DAB staining.
Immunohistochemical analysis of formalin-fixed and paraffin-embedded mouse intestine tissue labeling ANK-3 with ab213687 at 1/200 dilution, followed by conjugation to a secondary antibody and DAB staining.
ab213687 has been referenced in 1 publication.
- Radivojevic M et al. Electrical Identification and Selective Microstimulation of Neuronal Compartments Based on Features of Extracellular Action Potentials. Sci Rep 6:31332 (2016). ICC ; Rat . PubMed: 27510732