Overview

  • Product name

  • Description

    Rabbit polyclonal to ANK-3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ANK-3 aa 1060-1110 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    VIVEIPHFGSMRGKERELIVLRSENGETWKEHQFDSKNEDLTELLNGMDE E


    Database link: Q12955-3

  • Positive control

    • Mouse intestine and Rat brain tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab213687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.

When using a fluorescent probe the recommended dilution is 1/50 – 1/200.

Target

  • Function

    In skeletal muscle, required for costamere localization of DMD and betaDAG1 (By similarity). Membrane-cytoskeleton linker. May participate in the maintenance/targeting of ion channels and cell adhesion molecules at the nodes of Ranvier and axonal initial segments. Regulates KCNA1 channel activity in function of dietary Mg(2+) levels, and thereby contributes to the regulation of renal Mg(2+) reabsorption (PubMed:23903368).
    Isoform 5: May be part of a Golgi-specific membrane cytoskeleton in association with beta-spectrin.
  • Tissue specificity

    Expressed in brain, neurons, muscles and other tissues.
  • Involvement in disease

    Genetic variations in ANK3 may be associated with autism spectrum disorders susceptibility.
    Mental retardation, autosomal recessive 37
  • Sequence similarities

    Contains 23 ANK repeats.
    Contains 1 death domain.
    Contains 2 ZU5 domains.
  • Developmental stage

    Up-regulated during muscle cell differentiation.
  • Domain

    The tandem configuration of the two ZU5 and the UPA domains forms a structural supramodule termed ZZU. ZU5-1 mediates interaction with beta-spectrin, and the ZU5-1/UPA interface is required for ankyrin's function other than binding to spectrin.
  • Cellular localization

    Cytoplasm, cytoskeleton. Cell projection, axon. Cell membrane, sarcolemma. Cell junction, synapse, postsynaptic cell membrane. Lysosome. In skeletal muscle, localized at costameres and neuromuscular junctions. In macrophages, associated with lysosomes and Cytoplasm, cytoskeleton. Golgi apparatus.
  • Information by UniProt
  • Database links

  • Alternative names

    • ANK-3 antibody
    • ANK3 antibody
    • ANK3_HUMAN antibody
    • ankyrin 3 (G) antibody
    • Ankyrin 3, node of Ranvier (ankyrin G) antibody
    • Ankyrin 3, node of Ranvier antibody
    • Ankyrin G antibody
    • Ankyrin G119 antibody
    • Ankyrin-3 antibody
    • Ankyrin-G antibody
    • brain specific ankyrin G antibody
    • CHANK3 antibody
    • FLJ45464 antibody
    • OTTHUMP00000217458 antibody
    • OTTHUMP00000217575 antibody
    • RP11 369L1.1 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed and paraffin-embedded Rat brain tissue labeling ANK-3 with ab213687 at 1/200 dilution followed by Goat Anti-Rabbit IgG, Cy3 at 1/200 dilution for 40 minutes at 37°C.

  • Immunohistochemical analysis of formalin-fixed and paraffin-embedded Rat brain tissue labeling ANK-3 with ab213687 at 1/200 dilution, followed by conjugation to a secondary antibody and DAB staining.

  • Immunohistochemical analysis of formalin-fixed and paraffin-embedded mouse intestine tissue labeling ANK-3 with ab213687 at 1/200 dilution, followed by conjugation to a secondary antibody and DAB staining.

References

This product has been referenced in:

  • Radivojevic M  et al. Electrical Identification and Selective Microstimulation of Neuronal Compartments Based on Features of Extracellular Action Potentials. Sci Rep 6:31332 (2016). ICC ; Rat . Read more (PubMed: 27510732) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Feb 11 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Primary hippocampal neuronal cell culture)
Permeabilization
Yes - Together with blocking step: 30 min with PBS-Triton 0,3% and BSA 3%
Specification
Primary hippocampal neuronal cell culture
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Paraformaldehyde

Mrs. alanah pieters

Verified customer

Submitted Sep 08 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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