Overview

  • Product name

    Anti-ANK-3 antibody [N106/20]
    See all ANK-3 primary antibodies
  • Description

    Mouse monoclonal [N106/20] to ANK-3
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein corresponding to Human ANK-3 (C terminal). Corresponding to 1000 C-terminal amino acids of Human ANK-3, encompassing all of ANK-3 with the exception of Ankyrin repeats

  • Positive control

    • WB: Rat brain membrane lysate. ICC/IF: SK-N-BE cells.
  • General notes

    The clone number has been updated from S106-20 to N106/20, both clone numbers name the same antibody clone.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab134317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 480 kDa.

1µg/ml of ab134317 is sufficient for detection of Ankyrin G in 20µg of rat brain membrane lysate, assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

ICC/IF 1/100.

Target

  • Function

    In skeletal muscle, required for costamere localization of DMD and betaDAG1 (By similarity). Membrane-cytoskeleton linker. May participate in the maintenance/targeting of ion channels and cell adhesion molecules at the nodes of Ranvier and axonal initial segments. Regulates KCNA1 channel activity in function of dietary Mg(2+) levels, and thereby contributes to the regulation of renal Mg(2+) reabsorption (PubMed:23903368).
    Isoform 5: May be part of a Golgi-specific membrane cytoskeleton in association with beta-spectrin.
  • Tissue specificity

    Expressed in brain, neurons, muscles and other tissues.
  • Involvement in disease

    Genetic variations in ANK3 may be associated with autism spectrum disorders susceptibility.
    Mental retardation, autosomal recessive 37
  • Sequence similarities

    Contains 23 ANK repeats.
    Contains 1 death domain.
    Contains 2 ZU5 domains.
  • Developmental stage

    Up-regulated during muscle cell differentiation.
  • Domain

    The tandem configuration of the two ZU5 and the UPA domains forms a structural supramodule termed ZZU. ZU5-1 mediates interaction with beta-spectrin, and the ZU5-1/UPA interface is required for ankyrin's function other than binding to spectrin.
  • Cellular localization

    Cytoplasm, cytoskeleton. Cell projection, axon. Cell membrane, sarcolemma. Cell junction, synapse, postsynaptic cell membrane. Lysosome. In skeletal muscle, localized at costameres and neuromuscular junctions. In macrophages, associated with lysosomes and Cytoplasm, cytoskeleton. Golgi apparatus.
  • Information by UniProt
  • Database links

  • Alternative names

    • ANK-3 antibody
    • ANK3 antibody
    • ANK3_HUMAN antibody
    • ankyrin 3 (G) antibody
    • Ankyrin 3, node of Ranvier (ankyrin G) antibody
    • Ankyrin 3, node of Ranvier antibody
    • Ankyrin G antibody
    • Ankyrin G119 antibody
    • Ankyrin-3 antibody
    • Ankyrin-G antibody
    • brain specific ankyrin G antibody
    • CHANK3 antibody
    • FLJ45464 antibody
    • OTTHUMP00000217458 antibody
    • OTTHUMP00000217575 antibody
    • RP11 369L1.1 antibody
    see all

Images

  • 4% formaldehyde-fixed SK-N-BE (human) cells labeling ANK-3 using ab134317 at 1/100 dilution in ICC/IF. Cells were fixed for 15 minutes at room temperature and incubated with primary antibody for 1 hour at room temperature. Secondary antibody was a goat anti-mouse ATTO 488 (green) at 1/200 dilution incubated for 1 hour at rom temperature. Counterstained with Phalloidin Texas Red F-actin stain. Nuclei were stained with DAPI (blue).

  • Western blot detection of ANK-3 in Rat brain membrane tissues using ab134317 at 1:1000 dilution.

References

ab134317 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Cell (brain)
Permeabilization
Yes - T-PBS
Specification
brain
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

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Verified customer

Submitted Oct 10 2016

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