Anti-ANK-3 antibody [N106/20] (ab134317)
![Immunocytochemistry/ Immunofluorescence - Anti-ANK-3 antibody [N106/20] (ab134317)](https://www.abcam.com/ps/products/134/ab134317/Images/ab134317-311030-anti-ank-3-antibody-n106-20-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ANK-3 antibody [N106/20] (ab134317)")
Key features and details
- Mouse monoclonal [N106/20] to ANK-3
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-ANK-3 antibody [N106/20]
See all ANK-3 primary antibodies -
Description
Mouse monoclonal [N106/20] to ANK-3 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Fusion protein corresponding to Human ANK-3 (C terminal). Corresponding to 1000 C-terminal amino acids of Human ANK-3, encompassing all of ANK-3 with the exception of Ankyrin repeats
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Positive control
- WB: Rat brain membrane lysate. ICC/IF: SK-N-BE cells.
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General notes
The clone number has been updated from S106-20 to N106/20, both clone numbers name the same antibody clone.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N106/20 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab134317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 480 kDa. 1µg/ml of ab134317 is sufficient for detection of Ankyrin G in 20µg of rat brain membrane lysate, assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |
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| ICC/IF | 1/100. |
Target
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Function
In skeletal muscle, required for costamere localization of DMD and betaDAG1 (By similarity). Membrane-cytoskeleton linker. May participate in the maintenance/targeting of ion channels and cell adhesion molecules at the nodes of Ranvier and axonal initial segments. Regulates KCNA1 channel activity in function of dietary Mg(2+) levels, and thereby contributes to the regulation of renal Mg(2+) reabsorption (PubMed:23903368).
Isoform 5: May be part of a Golgi-specific membrane cytoskeleton in association with beta-spectrin. -
Tissue specificity
Expressed in brain, neurons, muscles and other tissues. -
Involvement in disease
Genetic variations in ANK3 may be associated with autism spectrum disorders susceptibility.
Mental retardation, autosomal recessive 37 -
Sequence similarities
Contains 23 ANK repeats.
Contains 1 death domain.
Contains 2 ZU5 domains. -
Developmental stage
Up-regulated during muscle cell differentiation. -
Domain
The tandem configuration of the two ZU5 and the UPA domains forms a structural supramodule termed ZZU. ZU5-1 mediates interaction with beta-spectrin, and the ZU5-1/UPA interface is required for ankyrin's function other than binding to spectrin. -
Cellular localization
Cytoplasm, cytoskeleton. Cell projection, axon. Cell membrane, sarcolemma. Cell junction, synapse, postsynaptic cell membrane. Lysosome. In skeletal muscle, localized at costameres and neuromuscular junctions. In macrophages, associated with lysosomes and Cytoplasm, cytoskeleton. Golgi apparatus. - Information by UniProt
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Database links
- Entrez Gene: 288 Human
- Entrez Gene: 11735 Mouse
- Entrez Gene: 361833 Rat
- Omim: 600465 Human
- SwissProt: Q12955 Human
- SwissProt: G5E8K5 Mouse
- SwissProt: O70511 Rat
- Unigene: 499725 Human
see all -
Alternative names
- ANK-3 antibody
- ANK3 antibody
- ANK3_HUMAN antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ANK-3 antibody [N106/20] (ab134317)
4% formaldehyde-fixed SK-N-BE (human) cells labeling ANK-3 using ab134317 at 1/100 dilution in ICC/IF. Cells were fixed for 15 minutes at room temperature and incubated with primary antibody for 1 hour at room temperature. Secondary antibody was a goat anti-mouse ATTO 488 (green) at 1/200 dilution incubated for 1 hour at rom temperature. Counterstained with Phalloidin Texas Red F-actin stain. Nuclei were stained with DAPI (blue).
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![Western blot - Anti-ANK-3 antibody [N106/20] (ab134317)](https://www.abcam.com/ps/products/134/ab134317/Images/ab134317-199712-anti-ank-3-antibody-n106-20-western-blot.jpg)
Western blot - Anti-ANK-3 antibody [N106/20] (ab134317)Western blot detection of ANK-3 in Rat brain membrane tissues using ab134317 at 1:1000 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab134317 has not yet been referenced specifically in any publications.
