Product nameAnti-ANKFY1 antibody - N-terminal
See all ANKFY1 primary antibodies
DescriptionRabbit polyclonal to ANKFY1 - N-terminal
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human ANKFY1 aa 1-50 (N terminal). The exact sequence is proprietary.
Database link: Q9P2R3
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226384 was affinity purified using an epitope specific to ANKFY1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab226384 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 128 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
Tissue specificityHigh expression in whole adult brain and intermediate expression in all other tissues and specific brain regions examined, including fetal brain.
Sequence similaritiesContains 21 ANK repeats.
Contains 1 BTB (POZ) domain.
Contains 1 FYVE-type zinc finger.
Cellular localizationCytoplasm. Endosome membrane. Also associated with endosomal membranes.
- Information by UniProt
- ANFY1_HUMAN antibody
- Ankfy1 antibody
- ANKHZN antibody
All lanes : Anti-ANKFY1 antibody - N-terminal (ab226384) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 128 kDa
Exposure time: 30 seconds
Lysates prepared using NETN lysis buffer.
ab226384 has not yet been referenced specifically in any publications.