Overview

  • Product name

    Anti-ANKFY1 antibody - N-terminal
    See all ANKFY1 primary antibodies
  • Description

    Rabbit polyclonal to ANKFY1 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human ANKFY1 aa 1-50 (N terminal). The exact sequence is proprietary.
    Database link: Q9P2R3

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab226384 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 128 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Tissue specificity

    High expression in whole adult brain and intermediate expression in all other tissues and specific brain regions examined, including fetal brain.
  • Sequence similarities

    Contains 21 ANK repeats.
    Contains 1 BTB (POZ) domain.
    Contains 1 FYVE-type zinc finger.
  • Cellular localization

    Cytoplasm. Endosome membrane. Also associated with endosomal membranes.
  • Information by UniProt
  • Database links

  • Alternative names

    • ANFY1_HUMAN antibody
    • Ankfy1 antibody
    • ANKHZN antibody
    • ankyrin repeat and FYVE domain containing 1 antibody
    • ankyrin repeat and FYVE domain containing protein 1 antibody
    • Ankyrin repeat and FYVE domain-containing protein 1 antibody
    • ankyrin repeat hooked to zinc finger motif antibody
    • Ankyrin repeats hooked to a zinc finger motif antibody
    • BTBD23 antibody
    • DKFZp686M19106 antibody
    • KIAA1255 antibody
    • Rabankyrin 5 antibody
    • ZFYVE14 antibody
    see all

Images

  • All lanes : Anti-ANKFY1 antibody - N-terminal (ab226384) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 128 kDa


    Exposure time: 30 seconds


    Lysates prepared using NETN lysis buffer.

References

ab226384 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab226384.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up