Key features and details
- Rabbit polyclonal to ANKFY1 - N-terminal
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ANKFY1 antibody - N-terminal
See all ANKFY1 primary antibodies
DescriptionRabbit polyclonal to ANKFY1 - N-terminal
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human ANKFY1 aa 1-50 (N terminal). The exact sequence is proprietary.
Database link: Q9P2R3
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226384 was affinity purified using an epitope specific to ANKFY1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab226384 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 128 kDa.|
Tissue specificityHigh expression in whole adult brain and intermediate expression in all other tissues and specific brain regions examined, including fetal brain.
Sequence similaritiesContains 21 ANK repeats.
Contains 1 BTB (POZ) domain.
Contains 1 FYVE-type zinc finger.
Cellular localizationCytoplasm. Endosome membrane. Also associated with endosomal membranes.
- Information by UniProt
- ANFY1_HUMAN antibody
- Ankfy1 antibody
- ANKHZN antibody
All lanes : Anti-ANKFY1 antibody - N-terminal (ab226384) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 128 kDa
Exposure time: 30 seconds
Lysates prepared using NETN lysis buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226384 has not yet been referenced specifically in any publications.