Anti-ANKH antibody - C-terminal (ab211460)
Key features and details
- Rabbit polyclonal to ANKH - C-terminal
- Suitable for: IHC-P, WB, Flow Cyt (Intra)
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ANKH antibody - C-terminal -
Description
Rabbit polyclonal to ANKH - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human ANKH aa 464-492 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary. NP_473368.1
Database link: Q9HCJ1 -
Positive control
- IHC-P: Human brain cortex tissue. Flow Cyt (Intra): K562 cells. WB: Mouse cerebellum tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab211460 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/10 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
1/1000. Predicted molecular weight: 54 kDa.
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Flow Cyt (Intra) |
1/10 - 1/50.
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Notes |
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IHC-P
1/10 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/1000. Predicted molecular weight: 54 kDa. |
Flow Cyt (Intra)
1/10 - 1/50. |
Target
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Function
Regulates intra- and extracellular levels of inorganic pyrophosphate (PPi), probably functioning as PPi transporter. -
Tissue specificity
Found in osteoblasts from mandibular bone and from iliac bone; not detected in osteoclastic cells. -
Involvement in disease
Chondrocalcinosis 2
Craniometaphyseal dysplasia, autosomal dominant -
Sequence similarities
Belongs to the ANKH family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 56172 Human
- Entrez Gene: 11732 Mouse
- Omim: 605145 Human
- SwissProt: Q9HCJ1 Human
- SwissProt: Q9JHZ2 Mouse
- Unigene: 156727 Human
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Alternative names
- ANK antibody
- ANKH antibody
- ANKH_HUMAN antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain cortex tissue labeling ANKH with ab211460 at 1/10 dilution.
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Intracellular flow cytometrical analysis of K562 cells labeling ANKH with ab211460 at 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Anti-ANKH antibody - C-terminal (ab211460) at 1/1000 dilution + Mouse cerebellum tissue lysate at 35 µg
Predicted band size: 54 kDa
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab211460 has not yet been referenced specifically in any publications.