Overview

  • Product name

    Anti-ANKH antibody [EPR8092]
    See all ANKH primary antibodies
  • Description

    Rabbit monoclonal [EPR8092] to ANKH
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in extracellular domain of Human ANKH (Q9HCJ1)

  • Positive control

    • Fetal brain or 293T lysate, 293T cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8092
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab129001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Regulates intra- and extracellular levels of inorganic pyrophosphate (PPi), probably functioning as PPi transporter.
    • Tissue specificity

      Found in osteoblasts from mandibular bone and from iliac bone; not detected in osteoclastic cells.
    • Involvement in disease

      Chondrocalcinosis 2
      Craniometaphyseal dysplasia, autosomal dominant
    • Sequence similarities

      Belongs to the ANKH family.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • ANK antibody
      • ANKH antibody
      • ANKH_HUMAN antibody
      • Ankylosis progressive homolog (mouse) antibody
      • Ankylosis progressive homolog antibody
      • CCAL 2 antibody
      • CCAL2 antibody
      • CMDJ antibody
      • CPPDD antibody
      • Craniometaphyseal dysplasia Jackson type (dominant) antibody
      • FLJ27166 antibody
      • HANK antibody
      • MANK antibody
      • Progressive ankylosis protein homolog antibody
      see all

    Images

    • All lanes : Anti-ANKH antibody [EPR8092] (ab129001) at 1/1000 dilution

      Lane 1 : Fetal brain cell lysate
      Lane 2 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat

      anti-rabbit at 1/2000 dilution

      Predicted band size: 54 kDa
      Observed band size: 54 kDa

    • Flow cytometric analysis of 293T cells using ab129001 at a 1/10 dilution (red), or a rabbit IgG (negative) (green).

    References

    ab129001 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab129001.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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