Anti-ANKHD1 antibody (ab117788)
Key features and details
- Rabbit polyclonal to ANKHD1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ANKHD1 antibody
See all ANKHD1 primary antibodies -
Description
Rabbit polyclonal to ANKHD1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Gorilla, Orangutan -
Immunogen
Synthetic peptide, corresponding to a region within amino acids 25-75 of Human ANKHD1 (NP_060217.1).
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Positive control
- HeLa and 293T whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab117788 was affinity purified using an epitope specific to ANKHD1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab117788 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 269 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 269 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
May play a role as a scaffolding protein that may be associated with the abnormal phenotype of leukemia cells. Isoform 2 may possess an antiapoptotic effect and protect cells during normal cell survival through its regulation of caspases. -
Tissue specificity
Ubiquitous with high expression in cervix, spleen and brain. Expressed in hematopoietic cells with increased expression in leukemia cells. Isoform 2 is highly expressed in spleen with almost no expression in muscle and brain. -
Sequence similarities
Belongs to the mask family.
Contains 25 ANK repeats.
Contains 1 KH domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 54882 Human
- Omim: 610500 Human
- SwissProt: Q8IWZ3 Human
- Unigene: 594084 Human
- Unigene: 720987 Human
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Alternative names
- ANKH1_HUMAN antibody
- ANKHD 1 antibody
- ANKHD1 antibody
see all
Images
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All lanes : Anti-ANKHD1 antibody (ab117788) at 0.1 µg/ml
Lane 1 : 293T whole cell lysates at 50 µg
Lane 2 : 293T whole cell lysates at 15 µg
Lane 3 : HeLa whole cell lysates at 50 µg
Developed using the ECL technique.
Predicted band size: 269 kDa
Exposure time: 30 seconds -
Detection of ANKHD1 by Western Blot of Immunprecipitate.
ab117788 at 1µg/ml staining ANKHD1 in 293T whole cell lysate immunoprecipitated using ab117788 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab117788 has been referenced in 2 publications.
- Lv HW et al. SMYD3 confers cisplatin chemoresistance of NSCLC cells in an ANKHD1-dependent manner. Transl Oncol 14:101075 (2021). PubMed: 33773404
- Liu XF et al. ANKHD1 promotes proliferation and invasion of non-small-cell lung cancer cells via regulating YAP oncoprotein expression and inactivating the Hippo pathway. Int J Oncol 56:1175-1185 (2020). PubMed: 32319569