Key features and details
- Rabbit polyclonal to ANKHD1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ANKHD1 antibody
See all ANKHD1 primary antibodies
DescriptionRabbit polyclonal to ANKHD1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 25-75 of Human ANKHD1 (NP_060217.1).
- HeLa and 293T whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab117788 was affinity purified using an epitope specific to ANKHD1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab117788 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 269 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionMay play a role as a scaffolding protein that may be associated with the abnormal phenotype of leukemia cells. Isoform 2 may possess an antiapoptotic effect and protect cells during normal cell survival through its regulation of caspases.
Tissue specificityUbiquitous with high expression in cervix, spleen and brain. Expressed in hematopoietic cells with increased expression in leukemia cells. Isoform 2 is highly expressed in spleen with almost no expression in muscle and brain.
Sequence similaritiesBelongs to the mask family.
Contains 25 ANK repeats.
Contains 1 KH domain.
- Information by UniProt
- ANKH1_HUMAN antibody
- ANKHD 1 antibody
- ANKHD1 antibody
All lanes : Anti-ANKHD1 antibody (ab117788) at 0.1 µg/ml
Lane 1 : 293T whole cell lysates at 50 µg
Lane 2 : 293T whole cell lysates at 15 µg
Lane 3 : HeLa whole cell lysates at 50 µg
Developed using the ECL technique.
Predicted band size: 269 kDa
Exposure time: 30 seconds
Detection of ANKHD1 by Western Blot of Immunprecipitate.
ab117788 at 1µg/ml staining ANKHD1 in 293T whole cell lysate immunoprecipitated using ab117788 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
ab117788 has been referenced in 1 publication.
- Liu XF et al. ANKHD1 promotes proliferation and invasion of non-small-cell lung cancer cells via regulating YAP oncoprotein expression and inactivating the Hippo pathway. Int J Oncol 56:1175-1185 (2020). PubMed: 32319569