Anti-ANKHD1 antibody - C-terminal (ab199164)
Key features and details
- Rabbit polyclonal to ANKHD1 - C-terminal
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ANKHD1 antibody - C-terminal
See all ANKHD1 primary antibodies -
Description
Rabbit polyclonal to ANKHD1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment (GST-tag) corresponding to Human ANKHD1 (C terminal). BC009420
Database link: Q8IWZ3-3 -
Positive control
- Human cervical cancer and Human thyroid cancer tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab199164 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
May play a role as a scaffolding protein that may be associated with the abnormal phenotype of leukemia cells. Isoform 2 may possess an antiapoptotic effect and protect cells during normal cell survival through its regulation of caspases. -
Tissue specificity
Ubiquitous with high expression in cervix, spleen and brain. Expressed in hematopoietic cells with increased expression in leukemia cells. Isoform 2 is highly expressed in spleen with almost no expression in muscle and brain. -
Sequence similarities
Belongs to the mask family.
Contains 25 ANK repeats.
Contains 1 KH domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 54882 Human
- Omim: 610500 Human
- SwissProt: Q8IWZ3 Human
- Unigene: 594084 Human
- Unigene: 720987 Human
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Alternative names
- ANKH1_HUMAN antibody
- ANKHD 1 antibody
- ANKHD1 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab199164 has been referenced in 1 publication.
- Liu XF et al. ANKHD1 promotes proliferation and invasion of non-small-cell lung cancer cells via regulating YAP oncoprotein expression and inactivating the Hippo pathway. Int J Oncol 56:1175-1185 (2020). PubMed: 32319569