Key features and details
- Rabbit polyclonal to ANKIB1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ANKIB1 antibody
DescriptionRabbit polyclonal to ANKIB1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Dog, Chimpanzee, Rhesus monkey, Gorilla, Elephant
Synthetic peptide, corresponding to a region between residue 1039 and 1089 of Human ANKIB1 (NP_061877.1)
- HeLa or 293T cell lysate
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab99346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 122 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionMight act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates.
Sequence similaritiesBelongs to the RBR family.
Contains 2 ANK repeats.
Contains 1 IBR-type zinc finger.
Contains 2 RING-type zinc fingers.
Contains 1 UIM (ubiquitin-interacting motif) repeat.
- Information by UniProt
- AKIB1_HUMAN antibody
- Ankib1 antibody
- ankyrin repeat and IBR domain containing 1 antibody
All lanes : Anti-ANKIB1 antibody (ab99346) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 122 kDa
Exposure time: 30 seconds
Lane 1: 3µg ab99346 were used to precipitate ANKIB1 from 3mg HeLa whole cell lysate. 20% IP was loaded on gel and probed with ab99346 at 1µg/ml.
Lane 2: same, except IP was carried with a control IgG.
Detection: chemiluminescence with exposure times of 3 seconds.
ab99346 has not yet been referenced specifically in any publications.