Product nameAnti-ANKIB1 antibody
DescriptionRabbit polyclonal to ANKIB1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Dog, Chimpanzee, Rhesus monkey, Gorilla, Elephant
Synthetic peptide, corresponding to a region between residue 1039 and 1089 of Human ANKIB1 (NP_061877.1)
- HeLa or 293T cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab99346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 122 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionMight act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates.
Sequence similaritiesBelongs to the RBR family.
Contains 2 ANK repeats.
Contains 1 IBR-type zinc finger.
Contains 2 RING-type zinc fingers.
Contains 1 UIM (ubiquitin-interacting motif) repeat.
- Information by UniProt
- AKIB1_HUMAN antibody
- Ankib1 antibody
- ankyrin repeat and IBR domain containing 1 antibody
All lanes : Anti-ANKIB1 antibody (ab99346) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 122 kDa
Exposure time: 30 seconds
Lane 1: 3µg ab99346 were used to precipitate ANKIB1 from 3mg HeLa whole cell lysate. 20% IP was loaded on gel and probed with ab99346 at 1µg/ml.
Lane 2: same, except IP was carried with a control IgG.
Detection: chemiluminescence with exposure times of 3 seconds.
ab99346 has not yet been referenced specifically in any publications.